Experiment: Methanol and casaminos (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3725 and GFF3726 are separated by 91 nucleotides GFF3726 and GFF3727 are separated by 9 nucleotides GFF3727 and GFF3728 overlap by 4 nucleotides GFF3728 and GFF3729 are separated by 80 nucleotides
GFF3725 - S-adenosylmethionine synthase, at 67,070 to 68,263
GFF3725
GFF3726 - tRNA (guanine-N(7)-)-methyltransferase, at 68,355 to 69,101
GFF3726
GFF3727 - hypothetical protein, at 69,111 to 69,977
GFF3727
GFF3728 - hypothetical protein, at 69,974 to 70,828
GFF3728
GFF3729 - Ferric uptake regulation protein, at 70,909 to 71,340
GFF3729
Position (kb)
69
70 Strain fitness (log2 ratio)
-2
-1
0
1 at 68.287 kb on + strand at 68.287 kb on + strand at 68.287 kb on + strand at 68.449 kb on - strand, within GFF3726 at 68.517 kb on + strand, within GFF3726 at 68.517 kb on + strand, within GFF3726 at 68.580 kb on + strand, within GFF3726 at 68.581 kb on - strand, within GFF3726 at 68.653 kb on + strand, within GFF3726 at 68.654 kb on - strand, within GFF3726 at 68.866 kb on + strand, within GFF3726 at 68.867 kb on - strand, within GFF3726 at 68.931 kb on + strand, within GFF3726 at 68.932 kb on - strand, within GFF3726 at 68.932 kb on - strand, within GFF3726 at 69.079 kb on - strand at 69.079 kb on - strand at 69.099 kb on + strand at 69.100 kb on - strand at 69.128 kb on - strand at 69.313 kb on + strand, within GFF3727 at 69.313 kb on + strand, within GFF3727 at 69.373 kb on + strand, within GFF3727 at 69.508 kb on + strand, within GFF3727 at 69.508 kb on + strand, within GFF3727 at 69.508 kb on + strand, within GFF3727 at 69.508 kb on + strand, within GFF3727 at 69.509 kb on - strand, within GFF3727 at 69.509 kb on - strand, within GFF3727 at 69.683 kb on - strand, within GFF3727 at 69.683 kb on - strand, within GFF3727 at 69.683 kb on - strand, within GFF3727 at 69.683 kb on - strand, within GFF3727 at 69.683 kb on - strand, within GFF3727 at 69.683 kb on - strand, within GFF3727 at 69.683 kb on - strand, within GFF3727 at 69.824 kb on + strand, within GFF3727 at 69.824 kb on + strand, within GFF3727 at 69.824 kb on + strand, within GFF3727 at 69.891 kb on - strand at 69.891 kb on - strand at 69.947 kb on - strand at 70.272 kb on + strand, within GFF3728 at 70.272 kb on + strand, within GFF3728 at 70.272 kb on + strand, within GFF3728 at 70.273 kb on - strand, within GFF3728 at 70.273 kb on - strand, within GFF3728 at 70.273 kb on - strand, within GFF3728 at 70.556 kb on + strand, within GFF3728 at 70.692 kb on + strand, within GFF3728 at 70.943 kb on + strand at 70.943 kb on + strand at 70.944 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Methanol and casaminos (C) remove 68,287 + -1.1 68,287 + +0.5 68,287 + +0.1 68,449 - GFF3726 0.13 +1.3 68,517 + GFF3726 0.22 -0.2 68,517 + GFF3726 0.22 +0.9 68,580 + GFF3726 0.30 +0.2 68,581 - GFF3726 0.30 -0.5 68,653 + GFF3726 0.40 +0.2 68,654 - GFF3726 0.40 -0.0 68,866 + GFF3726 0.68 +0.2 68,867 - GFF3726 0.69 -0.8 68,931 + GFF3726 0.77 +0.1 68,932 - GFF3726 0.77 +0.1 68,932 - GFF3726 0.77 -0.1 69,079 - -1.1 69,079 - -0.8 69,099 + -0.2 69,100 - -0.6 69,128 - +0.5 69,313 + GFF3727 0.23 +0.4 69,313 + GFF3727 0.23 +0.3 69,373 + GFF3727 0.30 +1.1 69,508 + GFF3727 0.46 +0.5 69,508 + GFF3727 0.46 +0.7 69,508 + GFF3727 0.46 +0.9 69,508 + GFF3727 0.46 -0.7 69,509 - GFF3727 0.46 +0.6 69,509 - GFF3727 0.46 -0.5 69,683 - GFF3727 0.66 +0.5 69,683 - GFF3727 0.66 +0.1 69,683 - GFF3727 0.66 +0.7 69,683 - GFF3727 0.66 +0.3 69,683 - GFF3727 0.66 -0.6 69,683 - GFF3727 0.66 +1.1 69,683 - GFF3727 0.66 -0.3 69,824 + GFF3727 0.82 +0.2 69,824 + GFF3727 0.82 +0.5 69,824 + GFF3727 0.82 +0.8 69,891 - -0.4 69,891 - +0.5 69,947 - +0.2 70,272 + GFF3728 0.35 -0.5 70,272 + GFF3728 0.35 -1.9 70,272 + GFF3728 0.35 +0.4 70,273 - GFF3728 0.35 +0.7 70,273 - GFF3728 0.35 +0.5 70,273 - GFF3728 0.35 -1.4 70,556 + GFF3728 0.68 -0.6 70,692 + GFF3728 0.84 +0.9 70,943 + -0.2 70,943 + -0.5 70,944 - +0.2
Or see this region's nucleotide sequence