Experiment: Methanol and casaminos (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3585 and GFF3586 are separated by 66 nucleotides GFF3586 and GFF3587 are separated by 27 nucleotides GFF3587 and GFF3588 are separated by 86 nucleotides GFF3588 and GFF3589 are separated by 15 nucleotides GFF3589 and GFF3590 are separated by 164 nucleotides
GFF3585 - hypothetical protein, at 447,368 to 448,408
GFF3585
GFF3586 - hypothetical protein, at 448,475 to 448,717
GFF3586
GFF3587 - hypothetical protein, at 448,745 to 449,299
GFF3587
GFF3588 - hypothetical protein, at 449,386 to 449,826
GFF3588
GFF3589 - Cysteine desulfuration protein SufE, at 449,842 to 450,264
GFF3589
GFF3590 - hypothetical protein, at 450,429 to 450,926
GFF3590
Position (kb) 449
450 Strain fitness (log2 ratio) -1
0
1 at 448.498 kb on + strand at 448.998 kb on - strand, within GFF3587 at 449.253 kb on - strand at 449.253 kb on - strand at 449.452 kb on + strand, within GFF3588 at 449.452 kb on + strand, within GFF3588 at 449.452 kb on + strand, within GFF3588 at 449.452 kb on + strand, within GFF3588 at 449.452 kb on + strand, within GFF3588 at 449.453 kb on - strand, within GFF3588 at 449.453 kb on - strand, within GFF3588 at 449.453 kb on - strand, within GFF3588 at 449.453 kb on - strand, within GFF3588 at 449.732 kb on - strand, within GFF3588 at 449.761 kb on + strand, within GFF3588 at 449.762 kb on - strand, within GFF3588 at 450.335 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Methanol and casaminos (C) remove 448,498 + -0.0 448,998 - GFF3587 0.46 -0.6 449,253 - +1.5 449,253 - -0.7 449,452 + GFF3588 0.15 +0.5 449,452 + GFF3588 0.15 +0.2 449,452 + GFF3588 0.15 -0.5 449,452 + GFF3588 0.15 +0.3 449,452 + GFF3588 0.15 -0.3 449,453 - GFF3588 0.15 -0.1 449,453 - GFF3588 0.15 +0.3 449,453 - GFF3588 0.15 +1.1 449,453 - GFF3588 0.15 +0.9 449,732 - GFF3588 0.78 -0.2 449,761 + GFF3588 0.85 -0.2 449,762 - GFF3588 0.85 +0.2 450,335 + -1.0
Or see this region's nucleotide sequence