Experiment: Methanol and casaminos (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3532 and GFF3533 are separated by 11 nucleotides GFF3533 and GFF3534 are separated by 17 nucleotides GFF3534 and GFF3535 are separated by 365 nucleotides
GFF3532 - Guanylate kinase, at 387,630 to 388,325
GFF3532
GFF3533 - hypothetical protein, at 388,337 to 389,281
GFF3533
GFF3534 - Endolytic murein transglycosylase, at 389,299 to 390,453
GFF3534
GFF3535 - 3-oxoacyl-[acyl-carrier-protein] synthase 2, at 390,819 to 392,081
GFF3535
Position (kb) 389
390
391 Strain fitness (log2 ratio) -2
-1
0
1 at 388.344 kb on - strand at 388.344 kb on - strand at 388.993 kb on + strand, within GFF3533 at 388.993 kb on + strand, within GFF3533 at 388.993 kb on + strand, within GFF3533 at 388.994 kb on - strand, within GFF3533 at 389.573 kb on + strand, within GFF3534 at 389.573 kb on + strand, within GFF3534 at 389.573 kb on + strand, within GFF3534 at 389.583 kb on - strand, within GFF3534 at 389.583 kb on - strand, within GFF3534 at 389.583 kb on - strand, within GFF3534 at 389.654 kb on + strand, within GFF3534 at 389.655 kb on - strand, within GFF3534 at 389.655 kb on - strand, within GFF3534 at 389.906 kb on + strand, within GFF3534 at 390.095 kb on + strand, within GFF3534 at 390.096 kb on - strand, within GFF3534 at 390.741 kb on - strand at 390.741 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Methanol and casaminos (C) remove 388,344 - +0.1 388,344 - -0.4 388,993 + GFF3533 0.69 +0.1 388,993 + GFF3533 0.69 +0.8 388,993 + GFF3533 0.69 -1.1 388,994 - GFF3533 0.70 -0.0 389,573 + GFF3534 0.24 +1.5 389,573 + GFF3534 0.24 +0.5 389,573 + GFF3534 0.24 +0.7 389,583 - GFF3534 0.25 +0.4 389,583 - GFF3534 0.25 +0.3 389,583 - GFF3534 0.25 -1.8 389,654 + GFF3534 0.31 +1.2 389,655 - GFF3534 0.31 +0.8 389,655 - GFF3534 0.31 -0.2 389,906 + GFF3534 0.53 +1.0 390,095 + GFF3534 0.69 +1.5 390,096 - GFF3534 0.69 +0.2 390,741 - +0.4 390,741 - +0.4
Or see this region's nucleotide sequence