Experiment: Methanol and casaminos (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3456 and GFF3457 are separated by 16 nucleotides GFF3457 and GFF3458 overlap by 4 nucleotides GFF3458 and GFF3459 are separated by 106 nucleotides
GFF3456 - High-affinity branched-chain amino acid transport ATP-binding protein LivF, at 307,728 to 308,432
GFF3456
GFF3457 - Shikimate dehydrogenase (NADP(+)), at 308,449 to 309,288
GFF3457
GFF3458 - NAD-dependent glycerol dehydrogenase, at 309,285 to 310,037
GFF3458
GFF3459 - 1-deoxy-D-xylulose-5-phosphate synthase, at 310,144 to 312,345
GFF3459
Position (kb) 309
310
311 Strain fitness (log2 ratio) -2
-1
0
1 at 308.340 kb on + strand, within GFF3456 at 308.340 kb on + strand, within GFF3456 at 308.341 kb on - strand, within GFF3456 at 308.418 kb on + strand at 308.506 kb on + strand at 308.506 kb on + strand at 308.507 kb on - strand at 308.507 kb on - strand at 308.507 kb on - strand at 308.507 kb on - strand at 308.512 kb on - strand at 308.512 kb on - strand at 309.015 kb on - strand, within GFF3457 at 309.495 kb on + strand, within GFF3458 at 309.702 kb on + strand, within GFF3458 at 309.702 kb on + strand, within GFF3458 at 309.702 kb on + strand, within GFF3458 at 309.702 kb on + strand, within GFF3458 at 309.702 kb on + strand, within GFF3458 at 309.703 kb on - strand, within GFF3458 at 309.703 kb on - strand, within GFF3458 at 309.703 kb on - strand, within GFF3458 at 309.832 kb on - strand, within GFF3458 at 309.832 kb on - strand, within GFF3458 at 310.023 kb on + strand at 310.024 kb on - strand at 310.192 kb on - strand at 310.694 kb on - strand, within GFF3459 at 310.716 kb on + strand, within GFF3459 at 310.795 kb on + strand, within GFF3459 at 310.795 kb on + strand, within GFF3459 at 310.796 kb on - strand, within GFF3459 at 310.796 kb on - strand, within GFF3459 at 310.981 kb on + strand, within GFF3459 at 310.981 kb on + strand, within GFF3459 at 310.982 kb on - strand, within GFF3459 at 310.982 kb on - strand, within GFF3459 at 310.982 kb on - strand, within GFF3459 at 310.987 kb on + strand, within GFF3459 at 310.987 kb on + strand, within GFF3459 at 310.987 kb on + strand, within GFF3459 at 310.987 kb on + strand, within GFF3459 at 310.988 kb on - strand, within GFF3459 at 310.988 kb on - strand, within GFF3459 at 310.988 kb on - strand, within GFF3459 at 311.030 kb on - strand, within GFF3459
Per-strain Table
Position Strand Gene LocusTag Fraction Methanol and casaminos (C) remove 308,340 + GFF3456 0.87 +0.2 308,340 + GFF3456 0.87 -0.3 308,341 - GFF3456 0.87 +1.4 308,418 + +1.7 308,506 + +0.6 308,506 + +0.3 308,507 - +0.1 308,507 - +1.1 308,507 - -0.6 308,507 - +0.4 308,512 - -0.4 308,512 - +0.8 309,015 - GFF3457 0.67 -0.5 309,495 + GFF3458 0.28 -0.4 309,702 + GFF3458 0.55 -0.2 309,702 + GFF3458 0.55 -0.9 309,702 + GFF3458 0.55 -0.2 309,702 + GFF3458 0.55 -0.5 309,702 + GFF3458 0.55 -0.5 309,703 - GFF3458 0.56 +1.1 309,703 - GFF3458 0.56 -0.3 309,703 - GFF3458 0.56 -0.6 309,832 - GFF3458 0.73 -1.1 309,832 - GFF3458 0.73 +0.7 310,023 + +0.2 310,024 - -0.4 310,192 - -0.1 310,694 - GFF3459 0.25 +0.8 310,716 + GFF3459 0.26 -0.2 310,795 + GFF3459 0.30 -0.5 310,795 + GFF3459 0.30 +0.1 310,796 - GFF3459 0.30 -0.2 310,796 - GFF3459 0.30 +0.3 310,981 + GFF3459 0.38 -0.6 310,981 + GFF3459 0.38 -0.1 310,982 - GFF3459 0.38 -0.5 310,982 - GFF3459 0.38 -0.4 310,982 - GFF3459 0.38 -0.0 310,987 + GFF3459 0.38 -0.0 310,987 + GFF3459 0.38 -0.1 310,987 + GFF3459 0.38 +0.1 310,987 + GFF3459 0.38 -0.8 310,988 - GFF3459 0.38 -0.7 310,988 - GFF3459 0.38 +0.3 310,988 - GFF3459 0.38 -2.5 311,030 - GFF3459 0.40 +1.3
Or see this region's nucleotide sequence