Experiment: Methanol and casaminos (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3452 and GFF3453 are separated by 279 nucleotides GFF3453 and GFF3454 are separated by 194 nucleotides
GFF3452 - Phthiocerol synthesis polyketide synthase type I PpsC, at 302,315 to 303,319
GFF3452
GFF3453 - Leu/Ile/Val-binding protein, at 303,599 to 304,828
GFF3453
GFF3454 - High-affinity branched-chain amino acid transport system permease protein LivH, at 305,023 to 305,895
GFF3454
Position (kb)
303
304
305 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 302.640 kb on + strand, within GFF3452 at 302.641 kb on - strand, within GFF3452 at 302.728 kb on - strand, within GFF3452 at 302.925 kb on + strand, within GFF3452 at 302.925 kb on + strand, within GFF3452 at 302.926 kb on - strand, within GFF3452 at 302.928 kb on + strand, within GFF3452 at 302.929 kb on - strand, within GFF3452 at 302.929 kb on - strand, within GFF3452 at 303.009 kb on + strand, within GFF3452 at 303.010 kb on - strand, within GFF3452 at 303.019 kb on - strand, within GFF3452 at 303.019 kb on - strand, within GFF3452 at 303.147 kb on + strand, within GFF3452 at 303.147 kb on + strand, within GFF3452 at 303.148 kb on - strand, within GFF3452 at 303.148 kb on - strand, within GFF3452 at 303.148 kb on - strand, within GFF3452 at 303.148 kb on - strand, within GFF3452 at 303.364 kb on - strand at 303.442 kb on + strand at 303.443 kb on - strand at 303.443 kb on - strand at 303.443 kb on - strand at 303.443 kb on - strand at 303.443 kb on - strand at 303.479 kb on - strand at 303.634 kb on + strand at 303.634 kb on + strand at 303.707 kb on + strand at 303.708 kb on - strand at 303.911 kb on + strand, within GFF3453 at 303.912 kb on - strand, within GFF3453 at 303.912 kb on - strand, within GFF3453 at 303.912 kb on - strand, within GFF3453 at 303.912 kb on - strand, within GFF3453 at 303.912 kb on - strand, within GFF3453 at 303.912 kb on - strand, within GFF3453 at 303.912 kb on - strand, within GFF3453 at 303.969 kb on - strand, within GFF3453 at 304.019 kb on + strand, within GFF3453 at 304.019 kb on + strand, within GFF3453 at 304.019 kb on + strand, within GFF3453 at 304.020 kb on - strand, within GFF3453 at 304.020 kb on - strand, within GFF3453 at 304.020 kb on - strand, within GFF3453 at 304.020 kb on - strand, within GFF3453 at 304.230 kb on - strand, within GFF3453 at 304.358 kb on + strand, within GFF3453 at 304.358 kb on + strand, within GFF3453 at 304.359 kb on - strand, within GFF3453 at 304.359 kb on - strand, within GFF3453 at 304.469 kb on + strand, within GFF3453 at 304.469 kb on + strand, within GFF3453 at 304.469 kb on + strand, within GFF3453 at 304.520 kb on + strand, within GFF3453 at 304.520 kb on + strand, within GFF3453 at 304.521 kb on - strand, within GFF3453 at 304.533 kb on - strand, within GFF3453 at 304.533 kb on - strand, within GFF3453 at 304.571 kb on + strand, within GFF3453 at 304.572 kb on - strand, within GFF3453 at 305.057 kb on + strand at 305.057 kb on + strand at 305.074 kb on + strand at 305.074 kb on + strand at 305.074 kb on + strand at 305.074 kb on + strand at 305.075 kb on - strand at 305.160 kb on + strand, within GFF3454 at 305.160 kb on + strand, within GFF3454 at 305.160 kb on + strand, within GFF3454 at 305.161 kb on - strand, within GFF3454 at 305.161 kb on - strand, within GFF3454 at 305.164 kb on + strand, within GFF3454 at 305.164 kb on + strand, within GFF3454 at 305.164 kb on + strand, within GFF3454 at 305.164 kb on + strand, within GFF3454 at 305.165 kb on - strand, within GFF3454 at 305.165 kb on - strand, within GFF3454 at 305.165 kb on - strand, within GFF3454 at 305.165 kb on - strand, within GFF3454 at 305.165 kb on - strand, within GFF3454 at 305.165 kb on - strand, within GFF3454 at 305.166 kb on - strand, within GFF3454 at 305.173 kb on + strand, within GFF3454 at 305.206 kb on + strand, within GFF3454 at 305.207 kb on - strand, within GFF3454 at 305.207 kb on - strand, within GFF3454 at 305.377 kb on + strand, within GFF3454 at 305.587 kb on + strand, within GFF3454 at 305.587 kb on + strand, within GFF3454 at 305.588 kb on - strand, within GFF3454 at 305.588 kb on - strand, within GFF3454 at 305.654 kb on - strand, within GFF3454 at 305.680 kb on + strand, within GFF3454 at 305.680 kb on + strand, within GFF3454 at 305.734 kb on + strand, within GFF3454 at 305.735 kb on - strand, within GFF3454 at 305.735 kb on - strand, within GFF3454 at 305.735 kb on - strand, within GFF3454 at 305.791 kb on + strand, within GFF3454 at 305.791 kb on + strand, within GFF3454 at 305.792 kb on - strand, within GFF3454 at 305.792 kb on - strand, within GFF3454 at 305.792 kb on - strand, within GFF3454 at 305.821 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Methanol and casaminos (C) remove 302,640 + GFF3452 0.32 +0.6 302,641 - GFF3452 0.32 -0.5 302,728 - GFF3452 0.41 +0.7 302,925 + GFF3452 0.61 -0.3 302,925 + GFF3452 0.61 -0.4 302,926 - GFF3452 0.61 -1.2 302,928 + GFF3452 0.61 +0.2 302,929 - GFF3452 0.61 +0.7 302,929 - GFF3452 0.61 -0.6 303,009 + GFF3452 0.69 -0.1 303,010 - GFF3452 0.69 -0.2 303,019 - GFF3452 0.70 +0.4 303,019 - GFF3452 0.70 +0.5 303,147 + GFF3452 0.83 +0.2 303,147 + GFF3452 0.83 +0.5 303,148 - GFF3452 0.83 -0.4 303,148 - GFF3452 0.83 +0.3 303,148 - GFF3452 0.83 -0.1 303,148 - GFF3452 0.83 +0.3 303,364 - -0.4 303,442 + -0.4 303,443 - -0.7 303,443 - -0.3 303,443 - -0.8 303,443 - +0.5 303,443 - +0.1 303,479 - -0.4 303,634 + +1.3 303,634 + -0.3 303,707 + +1.2 303,708 - -0.1 303,911 + GFF3453 0.25 +0.4 303,912 - GFF3453 0.25 +0.4 303,912 - GFF3453 0.25 -0.9 303,912 - GFF3453 0.25 +0.3 303,912 - GFF3453 0.25 -1.4 303,912 - GFF3453 0.25 +0.5 303,912 - GFF3453 0.25 +0.8 303,912 - GFF3453 0.25 +0.0 303,969 - GFF3453 0.30 -0.8 304,019 + GFF3453 0.34 +1.5 304,019 + GFF3453 0.34 -2.9 304,019 + GFF3453 0.34 +0.2 304,020 - GFF3453 0.34 +0.1 304,020 - GFF3453 0.34 +0.4 304,020 - GFF3453 0.34 +0.4 304,020 - GFF3453 0.34 +1.1 304,230 - GFF3453 0.51 -0.4 304,358 + GFF3453 0.62 -0.1 304,358 + GFF3453 0.62 +0.5 304,359 - GFF3453 0.62 -0.0 304,359 - GFF3453 0.62 -0.4 304,469 + GFF3453 0.71 -0.9 304,469 + GFF3453 0.71 -0.1 304,469 + GFF3453 0.71 +0.7 304,520 + GFF3453 0.75 -0.1 304,520 + GFF3453 0.75 -0.2 304,521 - GFF3453 0.75 -0.2 304,533 - GFF3453 0.76 -0.5 304,533 - GFF3453 0.76 +0.3 304,571 + GFF3453 0.79 -0.4 304,572 - GFF3453 0.79 +0.3 305,057 + +0.7 305,057 + +0.1 305,074 + +0.9 305,074 + -1.2 305,074 + -0.1 305,074 + +0.2 305,075 - +0.3 305,160 + GFF3454 0.16 +1.0 305,160 + GFF3454 0.16 -1.0 305,160 + GFF3454 0.16 -1.5 305,161 - GFF3454 0.16 -0.4 305,161 - GFF3454 0.16 +0.1 305,164 + GFF3454 0.16 +0.2 305,164 + GFF3454 0.16 +0.2 305,164 + GFF3454 0.16 -0.5 305,164 + GFF3454 0.16 -0.3 305,165 - GFF3454 0.16 +0.3 305,165 - GFF3454 0.16 -0.5 305,165 - GFF3454 0.16 +0.1 305,165 - GFF3454 0.16 +0.9 305,165 - GFF3454 0.16 -0.0 305,165 - GFF3454 0.16 +0.3 305,166 - GFF3454 0.16 +0.3 305,173 + GFF3454 0.17 +0.4 305,206 + GFF3454 0.21 -0.1 305,207 - GFF3454 0.21 -0.0 305,207 - GFF3454 0.21 +0.4 305,377 + GFF3454 0.41 -0.1 305,587 + GFF3454 0.65 -0.5 305,587 + GFF3454 0.65 -0.1 305,588 - GFF3454 0.65 +0.1 305,588 - GFF3454 0.65 +0.1 305,654 - GFF3454 0.72 +0.5 305,680 + GFF3454 0.75 +0.0 305,680 + GFF3454 0.75 +0.3 305,734 + GFF3454 0.81 -0.3 305,735 - GFF3454 0.82 +0.7 305,735 - GFF3454 0.82 +1.0 305,735 - GFF3454 0.82 -0.1 305,791 + GFF3454 0.88 +0.1 305,791 + GFF3454 0.88 +0.7 305,792 - GFF3454 0.88 -0.5 305,792 - GFF3454 0.88 -0.2 305,792 - GFF3454 0.88 +1.1 305,821 + +0.7
Or see this region's nucleotide sequence