Experiment: Methanol and casaminos (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3400 and GFF3401 are separated by 218 nucleotides GFF3401 and GFF3402 are separated by 88 nucleotides GFF3402 and GFF3403 are separated by 298 nucleotides
GFF3400 - hypothetical protein, at 244,356 to 244,850
GFF3400
GFF3401 - Protein YciF, at 245,069 to 245,560
GFF3401
GFF3402 - Nitrilotriacetate monooxygenase component A, at 245,649 to 247,010
GFF3402
GFF3403 - hypothetical protein, at 247,309 to 248,175
GFF3403
Position (kb)
245
246
247
248 Strain fitness (log2 ratio)
-1
0
1
2 at 244.683 kb on + strand, within GFF3400 at 245.123 kb on + strand, within GFF3401 at 245.124 kb on - strand, within GFF3401 at 245.124 kb on - strand, within GFF3401 at 245.126 kb on + strand, within GFF3401 at 245.402 kb on + strand, within GFF3401 at 245.402 kb on + strand, within GFF3401 at 245.403 kb on - strand, within GFF3401 at 245.403 kb on - strand, within GFF3401 at 245.650 kb on + strand at 245.651 kb on - strand at 245.743 kb on + strand at 245.743 kb on + strand at 245.744 kb on - strand at 245.744 kb on - strand at 245.944 kb on + strand, within GFF3402 at 245.944 kb on + strand, within GFF3402 at 245.944 kb on + strand, within GFF3402 at 245.944 kb on + strand, within GFF3402 at 245.944 kb on + strand, within GFF3402 at 245.944 kb on + strand, within GFF3402 at 245.944 kb on + strand, within GFF3402 at 246.055 kb on + strand, within GFF3402 at 246.129 kb on + strand, within GFF3402 at 246.129 kb on + strand, within GFF3402 at 246.130 kb on - strand, within GFF3402 at 246.130 kb on - strand, within GFF3402 at 246.130 kb on - strand, within GFF3402 at 246.215 kb on + strand, within GFF3402 at 246.215 kb on + strand, within GFF3402 at 246.215 kb on + strand, within GFF3402 at 246.253 kb on + strand, within GFF3402 at 246.254 kb on - strand, within GFF3402 at 246.254 kb on - strand, within GFF3402 at 246.457 kb on + strand, within GFF3402 at 246.551 kb on - strand, within GFF3402 at 246.728 kb on + strand, within GFF3402 at 246.729 kb on - strand, within GFF3402 at 246.729 kb on - strand, within GFF3402 at 247.084 kb on - strand at 247.087 kb on + strand at 247.088 kb on - strand at 247.088 kb on - strand at 247.088 kb on - strand at 247.463 kb on + strand, within GFF3403 at 247.463 kb on + strand, within GFF3403 at 247.464 kb on - strand, within GFF3403 at 247.499 kb on + strand, within GFF3403 at 247.499 kb on + strand, within GFF3403 at 247.500 kb on - strand, within GFF3403 at 247.500 kb on - strand, within GFF3403
Per-strain Table
Position Strand Gene LocusTag Fraction Methanol and casaminos (C) remove 244,683 + GFF3400 0.66 +0.1 245,123 + GFF3401 0.11 +0.2 245,124 - GFF3401 0.11 -0.4 245,124 - GFF3401 0.11 +0.5 245,126 + GFF3401 0.12 -0.1 245,402 + GFF3401 0.68 +0.0 245,402 + GFF3401 0.68 -1.1 245,403 - GFF3401 0.68 +0.6 245,403 - GFF3401 0.68 -0.1 245,650 + +0.0 245,651 - -0.1 245,743 + +0.1 245,743 + +0.2 245,744 - -0.6 245,744 - +0.8 245,944 + GFF3402 0.22 +0.4 245,944 + GFF3402 0.22 -0.3 245,944 + GFF3402 0.22 -0.8 245,944 + GFF3402 0.22 -0.1 245,944 + GFF3402 0.22 +0.2 245,944 + GFF3402 0.22 -0.9 245,944 + GFF3402 0.22 +0.3 246,055 + GFF3402 0.30 +0.0 246,129 + GFF3402 0.35 -0.2 246,129 + GFF3402 0.35 +0.4 246,130 - GFF3402 0.35 -0.3 246,130 - GFF3402 0.35 -0.8 246,130 - GFF3402 0.35 -0.1 246,215 + GFF3402 0.42 -0.1 246,215 + GFF3402 0.42 -0.0 246,215 + GFF3402 0.42 +1.8 246,253 + GFF3402 0.44 -0.9 246,254 - GFF3402 0.44 +0.6 246,254 - GFF3402 0.44 -0.8 246,457 + GFF3402 0.59 +0.2 246,551 - GFF3402 0.66 -0.4 246,728 + GFF3402 0.79 -0.0 246,729 - GFF3402 0.79 -0.1 246,729 - GFF3402 0.79 +2.1 247,084 - -0.8 247,087 + +0.4 247,088 - +0.2 247,088 - +0.1 247,088 - +0.5 247,463 + GFF3403 0.18 -0.3 247,463 + GFF3403 0.18 -0.8 247,464 - GFF3403 0.18 +0.5 247,499 + GFF3403 0.22 -1.1 247,499 + GFF3403 0.22 -0.1 247,500 - GFF3403 0.22 +0.8 247,500 - GFF3403 0.22 -0.8
Or see this region's nucleotide sequence