Experiment: Methanol and casaminos (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3368 and GFF3369 are separated by 166 nucleotides GFF3369 and GFF3370 are separated by 270 nucleotides GFF3370 and GFF3371 overlap by 4 nucleotides
GFF3368 - L-lactate dehydrogenase, at 212,998 to 214,188
GFF3368
GFF3369 - hypothetical protein, at 214,355 to 215,344
GFF3369
GFF3370 - Low-molecular weight cobalt-containing nitrile hydratase subunit alpha, at 215,615 to 216,238
GFF3370
GFF3371 - hypothetical protein, at 216,235 to 216,537
GFF3371
Position (kb) 214
215
216 Strain fitness (log2 ratio) -1
0
1
2 at 214.103 kb on + strand at 214.103 kb on + strand at 214.109 kb on + strand at 214.109 kb on + strand at 214.246 kb on + strand at 214.421 kb on + strand at 214.422 kb on - strand at 214.510 kb on + strand, within GFF3369 at 214.511 kb on - strand, within GFF3369 at 214.670 kb on + strand, within GFF3369 at 214.670 kb on + strand, within GFF3369 at 214.671 kb on - strand, within GFF3369 at 214.671 kb on - strand, within GFF3369 at 214.671 kb on - strand, within GFF3369 at 214.671 kb on - strand, within GFF3369 at 214.877 kb on + strand, within GFF3369 at 214.878 kb on - strand, within GFF3369 at 215.051 kb on + strand, within GFF3369 at 215.156 kb on - strand, within GFF3369 at 215.156 kb on - strand, within GFF3369 at 215.322 kb on - strand at 215.492 kb on + strand at 215.492 kb on + strand at 215.492 kb on + strand at 215.616 kb on + strand at 215.616 kb on + strand at 215.616 kb on + strand at 215.727 kb on + strand, within GFF3370 at 215.727 kb on + strand, within GFF3370 at 215.727 kb on + strand, within GFF3370 at 215.728 kb on - strand, within GFF3370 at 215.850 kb on + strand, within GFF3370 at 215.850 kb on + strand, within GFF3370 at 215.850 kb on + strand, within GFF3370 at 215.850 kb on + strand, within GFF3370 at 215.850 kb on + strand, within GFF3370 at 215.850 kb on + strand, within GFF3370 at 215.851 kb on - strand, within GFF3370 at 215.851 kb on - strand, within GFF3370 at 215.851 kb on - strand, within GFF3370 at 215.851 kb on - strand, within GFF3370 at 215.917 kb on + strand, within GFF3370 at 215.918 kb on - strand, within GFF3370 at 215.932 kb on + strand, within GFF3370 at 216.171 kb on + strand, within GFF3370 at 216.175 kb on - strand, within GFF3370 at 216.314 kb on + strand, within GFF3371
Per-strain Table
Position Strand Gene LocusTag Fraction Methanol and casaminos (C) remove 214,103 + +1.3 214,103 + +0.5 214,109 + +0.2 214,109 + +0.5 214,246 + -1.3 214,421 + -0.2 214,422 - -0.2 214,510 + GFF3369 0.16 +0.5 214,511 - GFF3369 0.16 +1.5 214,670 + GFF3369 0.32 -0.9 214,670 + GFF3369 0.32 -0.5 214,671 - GFF3369 0.32 +0.7 214,671 - GFF3369 0.32 -1.0 214,671 - GFF3369 0.32 -0.8 214,671 - GFF3369 0.32 +0.2 214,877 + GFF3369 0.53 +0.2 214,878 - GFF3369 0.53 -0.8 215,051 + GFF3369 0.70 +0.5 215,156 - GFF3369 0.81 -0.3 215,156 - GFF3369 0.81 -1.7 215,322 - +0.3 215,492 + -0.3 215,492 + -0.6 215,492 + -0.1 215,616 + +0.9 215,616 + +0.1 215,616 + -0.7 215,727 + GFF3370 0.18 +0.2 215,727 + GFF3370 0.18 -0.3 215,727 + GFF3370 0.18 +0.6 215,728 - GFF3370 0.18 +0.7 215,850 + GFF3370 0.38 -0.0 215,850 + GFF3370 0.38 +0.1 215,850 + GFF3370 0.38 -0.5 215,850 + GFF3370 0.38 +1.1 215,850 + GFF3370 0.38 +0.8 215,850 + GFF3370 0.38 +0.2 215,851 - GFF3370 0.38 -0.3 215,851 - GFF3370 0.38 +0.8 215,851 - GFF3370 0.38 -0.1 215,851 - GFF3370 0.38 +1.0 215,917 + GFF3370 0.48 -0.5 215,918 - GFF3370 0.49 +0.2 215,932 + GFF3370 0.51 -0.4 216,171 + GFF3370 0.89 +2.0 216,175 - GFF3370 0.90 +0.1 216,314 + GFF3371 0.26 +0.6
Or see this region's nucleotide sequence