Experiment: Methanol and casaminos (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3338 and GFF3339 are separated by 145 nucleotides GFF3339 and GFF3340 are separated by 95 nucleotides GFF3340 and GFF3341 are separated by 44 nucleotides GFF3341 and GFF3342 are separated by 142 nucleotides
GFF3338 - Beta-alanine--pyruvate aminotransferase, at 179,981 to 181,315
GFF3338
GFF3339 - HTH-type transcriptional regulator PuuR, at 181,461 to 182,003
GFF3339
GFF3340 - putative acrylyl-CoA reductase AcuI, at 182,099 to 183,085
GFF3340
GFF3341 - Pyruvate dehydrogenase complex repressor, at 183,130 to 183,900
GFF3341
GFF3342 - hypothetical protein, at 184,043 to 185,293
GFF3342
Position (kb) 182
183
184 Strain fitness (log2 ratio) -2
-1
0
1 at 181.197 kb on + strand at 181.197 kb on + strand at 181.197 kb on + strand at 181.200 kb on + strand at 181.200 kb on + strand at 181.201 kb on - strand at 181.201 kb on - strand at 181.201 kb on - strand at 181.201 kb on - strand at 181.201 kb on - strand at 181.201 kb on - strand at 181.574 kb on + strand, within GFF3339 at 181.574 kb on + strand, within GFF3339 at 181.575 kb on - strand, within GFF3339 at 181.575 kb on - strand, within GFF3339 at 181.575 kb on - strand, within GFF3339 at 181.680 kb on + strand, within GFF3339 at 181.680 kb on + strand, within GFF3339 at 181.681 kb on - strand, within GFF3339 at 181.681 kb on - strand, within GFF3339 at 181.681 kb on - strand, within GFF3339 at 181.681 kb on - strand, within GFF3339 at 181.681 kb on - strand, within GFF3339 at 181.726 kb on - strand, within GFF3339 at 181.782 kb on + strand, within GFF3339 at 181.783 kb on - strand, within GFF3339 at 181.783 kb on - strand, within GFF3339 at 181.911 kb on + strand, within GFF3339 at 181.911 kb on + strand, within GFF3339 at 181.912 kb on - strand, within GFF3339 at 181.912 kb on - strand, within GFF3339 at 181.912 kb on - strand, within GFF3339 at 181.914 kb on + strand, within GFF3339 at 181.914 kb on + strand, within GFF3339 at 181.914 kb on + strand, within GFF3339 at 181.915 kb on - strand, within GFF3339 at 181.915 kb on - strand, within GFF3339 at 182.472 kb on + strand, within GFF3340 at 182.650 kb on - strand, within GFF3340 at 182.949 kb on + strand, within GFF3340 at 182.950 kb on - strand, within GFF3340 at 182.950 kb on - strand, within GFF3340 at 182.950 kb on - strand, within GFF3340 at 182.964 kb on + strand, within GFF3340 at 182.964 kb on + strand, within GFF3340 at 183.170 kb on + strand at 183.171 kb on - strand at 183.185 kb on + strand at 183.185 kb on + strand at 183.185 kb on + strand at 183.186 kb on - strand at 183.712 kb on + strand, within GFF3341 at 183.712 kb on + strand, within GFF3341
Per-strain Table
Position Strand Gene LocusTag Fraction Methanol and casaminos (C) remove 181,197 + +0.6 181,197 + -0.4 181,197 + +0.9 181,200 + +0.2 181,200 + -1.3 181,201 - -0.4 181,201 - +1.6 181,201 - -0.2 181,201 - +0.1 181,201 - +1.0 181,201 - +0.7 181,574 + GFF3339 0.21 +0.0 181,574 + GFF3339 0.21 +0.2 181,575 - GFF3339 0.21 -1.2 181,575 - GFF3339 0.21 -1.8 181,575 - GFF3339 0.21 -0.1 181,680 + GFF3339 0.40 -0.1 181,680 + GFF3339 0.40 -0.8 181,681 - GFF3339 0.41 +0.0 181,681 - GFF3339 0.41 -0.9 181,681 - GFF3339 0.41 +0.5 181,681 - GFF3339 0.41 +1.2 181,681 - GFF3339 0.41 -0.8 181,726 - GFF3339 0.49 -0.2 181,782 + GFF3339 0.59 -0.7 181,783 - GFF3339 0.59 -0.4 181,783 - GFF3339 0.59 +0.2 181,911 + GFF3339 0.83 -0.9 181,911 + GFF3339 0.83 +0.6 181,912 - GFF3339 0.83 +0.0 181,912 - GFF3339 0.83 +0.3 181,912 - GFF3339 0.83 +0.3 181,914 + GFF3339 0.83 -0.1 181,914 + GFF3339 0.83 +0.1 181,914 + GFF3339 0.83 +0.1 181,915 - GFF3339 0.84 -0.7 181,915 - GFF3339 0.84 -0.1 182,472 + GFF3340 0.38 -0.1 182,650 - GFF3340 0.56 +0.1 182,949 + GFF3340 0.86 -0.8 182,950 - GFF3340 0.86 +0.0 182,950 - GFF3340 0.86 -0.3 182,950 - GFF3340 0.86 +0.1 182,964 + GFF3340 0.88 -0.7 182,964 + GFF3340 0.88 +0.1 183,170 + -0.2 183,171 - -0.5 183,185 + -0.6 183,185 + -0.0 183,185 + -0.1 183,186 - -0.1 183,712 + GFF3341 0.75 +0.1 183,712 + GFF3341 0.75 +0.2
Or see this region's nucleotide sequence