Experiment: Methanol and casaminos (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3195 and GFF3196 are separated by 114 nucleotides GFF3196 and GFF3197 overlap by 4 nucleotides GFF3197 and GFF3198 overlap by 4 nucleotides GFF3198 and GFF3199 are separated by 41 nucleotides
GFF3195 - Leu/Ile/Val-binding protein, at 19,029 to 20,231
GFF3195
GFF3196 - High-affinity branched-chain amino acid transport system permease protein LivH, at 20,346 to 21,221
GFF3196
GFF3197 - Vitamin B12 import ATP-binding protein BtuD, at 21,218 to 23,026
GFF3197
GFF3198 - High-affinity branched-chain amino acid transport ATP-binding protein LivF, at 23,023 to 23,781
GFF3198
GFF3199 - Kynurenine formamidase, at 23,823 to 24,518
GFF3199
Position (kb)
21
22
23
24 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 20.221 kb on - strand at 20.364 kb on + strand at 20.364 kb on + strand at 20.364 kb on + strand at 20.364 kb on + strand at 20.364 kb on + strand at 20.364 kb on + strand at 20.364 kb on + strand at 20.365 kb on - strand at 20.365 kb on - strand at 20.365 kb on - strand at 20.365 kb on - strand at 20.397 kb on + strand at 20.398 kb on - strand at 20.398 kb on - strand at 20.398 kb on - strand at 20.499 kb on + strand, within GFF3196 at 20.499 kb on + strand, within GFF3196 at 20.499 kb on + strand, within GFF3196 at 20.500 kb on - strand, within GFF3196 at 20.694 kb on + strand, within GFF3196 at 21.115 kb on - strand, within GFF3196 at 21.115 kb on - strand, within GFF3196 at 21.115 kb on - strand, within GFF3196 at 21.115 kb on - strand, within GFF3196 at 21.115 kb on - strand, within GFF3196 at 21.285 kb on + strand at 21.286 kb on - strand at 21.308 kb on + strand at 21.309 kb on - strand at 21.341 kb on + strand at 21.341 kb on + strand at 21.341 kb on + strand at 21.348 kb on + strand at 21.348 kb on + strand at 21.349 kb on - strand at 21.349 kb on - strand at 21.357 kb on + strand at 21.431 kb on + strand, within GFF3197 at 21.431 kb on + strand, within GFF3197 at 21.531 kb on - strand, within GFF3197 at 21.535 kb on + strand, within GFF3197 at 21.535 kb on + strand, within GFF3197 at 21.536 kb on - strand, within GFF3197 at 21.536 kb on - strand, within GFF3197 at 21.536 kb on - strand, within GFF3197 at 21.557 kb on + strand, within GFF3197 at 21.557 kb on + strand, within GFF3197 at 21.558 kb on - strand, within GFF3197 at 21.558 kb on - strand, within GFF3197 at 21.558 kb on - strand, within GFF3197 at 21.558 kb on - strand, within GFF3197 at 21.692 kb on + strand, within GFF3197 at 21.692 kb on + strand, within GFF3197 at 21.701 kb on + strand, within GFF3197 at 21.701 kb on + strand, within GFF3197 at 21.701 kb on + strand, within GFF3197 at 21.702 kb on - strand, within GFF3197 at 21.702 kb on - strand, within GFF3197 at 21.702 kb on - strand, within GFF3197 at 21.702 kb on - strand, within GFF3197 at 21.702 kb on - strand, within GFF3197 at 21.896 kb on + strand, within GFF3197 at 21.897 kb on - strand, within GFF3197 at 21.897 kb on - strand, within GFF3197 at 21.897 kb on - strand, within GFF3197 at 21.914 kb on + strand, within GFF3197 at 21.915 kb on - strand, within GFF3197 at 21.932 kb on + strand, within GFF3197 at 22.016 kb on + strand, within GFF3197 at 22.017 kb on - strand, within GFF3197 at 22.017 kb on - strand, within GFF3197 at 22.028 kb on + strand, within GFF3197 at 22.028 kb on + strand, within GFF3197 at 22.029 kb on - strand, within GFF3197 at 22.029 kb on - strand, within GFF3197 at 22.076 kb on + strand, within GFF3197 at 22.326 kb on + strand, within GFF3197 at 22.326 kb on + strand, within GFF3197 at 22.327 kb on - strand, within GFF3197 at 22.327 kb on - strand, within GFF3197 at 22.327 kb on - strand, within GFF3197 at 22.327 kb on - strand, within GFF3197 at 22.327 kb on - strand, within GFF3197 at 22.391 kb on + strand, within GFF3197 at 22.392 kb on - strand, within GFF3197 at 22.644 kb on + strand, within GFF3197 at 22.644 kb on + strand, within GFF3197 at 22.664 kb on + strand, within GFF3197 at 22.665 kb on - strand, within GFF3197 at 22.910 kb on + strand at 22.910 kb on + strand at 22.911 kb on - strand at 22.911 kb on - strand at 22.976 kb on + strand at 22.977 kb on - strand at 22.977 kb on - strand at 22.977 kb on - strand at 22.977 kb on - strand at 22.977 kb on - strand at 22.977 kb on - strand at 22.977 kb on - strand at 23.078 kb on - strand at 23.086 kb on + strand at 23.087 kb on - strand at 23.359 kb on + strand, within GFF3198 at 23.360 kb on - strand, within GFF3198 at 23.360 kb on - strand, within GFF3198 at 23.648 kb on - strand, within GFF3198 at 23.674 kb on + strand, within GFF3198 at 23.674 kb on + strand, within GFF3198 at 23.674 kb on + strand, within GFF3198 at 23.674 kb on + strand, within GFF3198 at 23.675 kb on - strand, within GFF3198 at 23.675 kb on - strand, within GFF3198 at 23.675 kb on - strand, within GFF3198 at 23.675 kb on - strand, within GFF3198
Per-strain Table
Position Strand Gene LocusTag Fraction Methanol and casaminos (C) remove 20,221 - -1.1 20,364 + +0.2 20,364 + +1.0 20,364 + +0.7 20,364 + +0.8 20,364 + +0.3 20,364 + +0.4 20,364 + +0.5 20,365 - +1.3 20,365 - +0.2 20,365 - +0.8 20,365 - -0.1 20,397 + +0.0 20,398 - +0.2 20,398 - +0.7 20,398 - -0.7 20,499 + GFF3196 0.17 -0.6 20,499 + GFF3196 0.17 -0.4 20,499 + GFF3196 0.17 +0.1 20,500 - GFF3196 0.18 -1.3 20,694 + GFF3196 0.40 -0.3 21,115 - GFF3196 0.88 -0.3 21,115 - GFF3196 0.88 -0.6 21,115 - GFF3196 0.88 +0.0 21,115 - GFF3196 0.88 -1.0 21,115 - GFF3196 0.88 -0.2 21,285 + +0.8 21,286 - +0.5 21,308 + -0.3 21,309 - -1.1 21,341 + +0.8 21,341 + -0.2 21,341 + -0.3 21,348 + +0.2 21,348 + -1.1 21,349 - +1.2 21,349 - +0.4 21,357 + +1.2 21,431 + GFF3197 0.12 +0.6 21,431 + GFF3197 0.12 -0.8 21,531 - GFF3197 0.17 -0.1 21,535 + GFF3197 0.18 +0.1 21,535 + GFF3197 0.18 -0.6 21,536 - GFF3197 0.18 +0.7 21,536 - GFF3197 0.18 -0.3 21,536 - GFF3197 0.18 -0.7 21,557 + GFF3197 0.19 -1.0 21,557 + GFF3197 0.19 +0.4 21,558 - GFF3197 0.19 -0.6 21,558 - GFF3197 0.19 +0.8 21,558 - GFF3197 0.19 -0.2 21,558 - GFF3197 0.19 -1.1 21,692 + GFF3197 0.26 -1.8 21,692 + GFF3197 0.26 -0.1 21,701 + GFF3197 0.27 +0.7 21,701 + GFF3197 0.27 +0.3 21,701 + GFF3197 0.27 -0.6 21,702 - GFF3197 0.27 -0.5 21,702 - GFF3197 0.27 -0.7 21,702 - GFF3197 0.27 +0.0 21,702 - GFF3197 0.27 +0.7 21,702 - GFF3197 0.27 -0.5 21,896 + GFF3197 0.37 -0.1 21,897 - GFF3197 0.38 -0.3 21,897 - GFF3197 0.38 -0.6 21,897 - GFF3197 0.38 +1.2 21,914 + GFF3197 0.38 +0.3 21,915 - GFF3197 0.39 +0.5 21,932 + GFF3197 0.39 -2.6 22,016 + GFF3197 0.44 +0.6 22,017 - GFF3197 0.44 -0.4 22,017 - GFF3197 0.44 -1.2 22,028 + GFF3197 0.45 -0.2 22,028 + GFF3197 0.45 -0.2 22,029 - GFF3197 0.45 +0.5 22,029 - GFF3197 0.45 +0.6 22,076 + GFF3197 0.47 -0.8 22,326 + GFF3197 0.61 -0.0 22,326 + GFF3197 0.61 -1.7 22,327 - GFF3197 0.61 +2.3 22,327 - GFF3197 0.61 -0.8 22,327 - GFF3197 0.61 +0.4 22,327 - GFF3197 0.61 -1.2 22,327 - GFF3197 0.61 -2.5 22,391 + GFF3197 0.65 -0.5 22,392 - GFF3197 0.65 -0.1 22,644 + GFF3197 0.79 +0.0 22,644 + GFF3197 0.79 -1.0 22,664 + GFF3197 0.80 -0.2 22,665 - GFF3197 0.80 +0.8 22,910 + -0.0 22,910 + +0.0 22,911 - +0.1 22,911 - +0.8 22,976 + -0.3 22,977 - +1.6 22,977 - -0.5 22,977 - -1.4 22,977 - +0.5 22,977 - +0.4 22,977 - +0.7 22,977 - +0.4 23,078 - -0.0 23,086 + -0.0 23,087 - -1.3 23,359 + GFF3198 0.44 -0.1 23,360 - GFF3198 0.44 -0.5 23,360 - GFF3198 0.44 -0.1 23,648 - GFF3198 0.82 -0.2 23,674 + GFF3198 0.86 -0.6 23,674 + GFF3198 0.86 -0.0 23,674 + GFF3198 0.86 -0.1 23,674 + GFF3198 0.86 -0.3 23,675 - GFF3198 0.86 +0.5 23,675 - GFF3198 0.86 +0.2 23,675 - GFF3198 0.86 -0.7 23,675 - GFF3198 0.86 +1.3
Or see this region's nucleotide sequence