Experiment: Methanol and casaminos (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3007 and GFF3008 are separated by 83 nucleotides GFF3008 and GFF3009 are separated by 67 nucleotides GFF3009 and GFF3010 are separated by 162 nucleotides GFF3010 and GFF3011 are separated by 9 nucleotides
GFF3007 - Short-chain-enoyl-CoA hydratase, at 733,211 to 734,032
GFF3007
GFF3008 - hypothetical protein, at 734,116 to 734,817
GFF3008
GFF3009 - 1-deoxyxylulose-5-phosphate synthase YajO, at 734,885 to 735,859
GFF3009
GFF3010 - hypothetical protein, at 736,022 to 736,180
GFF3010
GFF3011 - Pimeloyl-[acyl-carrier protein] methyl ester esterase, at 736,190 to 737,011
GFF3011
Position (kb) 734
735
736 Strain fitness (log2 ratio) -1
0
1 at 733.935 kb on + strand, within GFF3007 at 733.936 kb on - strand, within GFF3007 at 734.087 kb on - strand at 734.105 kb on - strand at 734.278 kb on + strand, within GFF3008 at 734.351 kb on - strand, within GFF3008 at 734.390 kb on - strand, within GFF3008 at 734.390 kb on - strand, within GFF3008 at 734.390 kb on - strand, within GFF3008 at 734.390 kb on - strand, within GFF3008 at 734.891 kb on + strand at 734.892 kb on - strand at 734.995 kb on + strand, within GFF3009 at 735.066 kb on - strand, within GFF3009 at 735.248 kb on + strand, within GFF3009 at 735.248 kb on + strand, within GFF3009 at 735.249 kb on - strand, within GFF3009 at 735.249 kb on - strand, within GFF3009 at 735.260 kb on + strand, within GFF3009 at 735.260 kb on + strand, within GFF3009 at 735.396 kb on - strand, within GFF3009 at 735.514 kb on + strand, within GFF3009 at 735.608 kb on + strand, within GFF3009 at 735.824 kb on + strand at 735.824 kb on + strand at 735.824 kb on + strand at 735.825 kb on - strand at 735.825 kb on - strand at 735.825 kb on - strand at 735.825 kb on - strand at 735.825 kb on - strand at 735.825 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Methanol and casaminos (C) remove 733,935 + GFF3007 0.88 +0.5 733,936 - GFF3007 0.88 +0.1 734,087 - +0.3 734,105 - +0.7 734,278 + GFF3008 0.23 +0.2 734,351 - GFF3008 0.33 -0.2 734,390 - GFF3008 0.39 +0.4 734,390 - GFF3008 0.39 +0.4 734,390 - GFF3008 0.39 -0.5 734,390 - GFF3008 0.39 +0.3 734,891 + -0.3 734,892 - -0.6 734,995 + GFF3009 0.11 -0.3 735,066 - GFF3009 0.19 -0.7 735,248 + GFF3009 0.37 -0.5 735,248 + GFF3009 0.37 -0.7 735,249 - GFF3009 0.37 -0.6 735,249 - GFF3009 0.37 -0.0 735,260 + GFF3009 0.38 -0.4 735,260 + GFF3009 0.38 +0.0 735,396 - GFF3009 0.52 -0.2 735,514 + GFF3009 0.65 +0.1 735,608 + GFF3009 0.74 -0.0 735,824 + +0.2 735,824 + +0.3 735,824 + -0.2 735,825 - +0.4 735,825 - -0.5 735,825 - -0.0 735,825 - +0.5 735,825 - +0.9 735,825 - -0.8
Or see this region's nucleotide sequence