Experiment: Methanol and casaminos (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3005 and GFF3006 are separated by 37 nucleotides GFF3006 and GFF3007 are separated by 3 nucleotides GFF3007 and GFF3008 are separated by 83 nucleotides
GFF3005 - Glycine cleavage system transcriptional activator, at 731,848 to 732,750
GFF3005
GFF3006 - hypothetical protein, at 732,788 to 733,207
GFF3006
GFF3007 - Short-chain-enoyl-CoA hydratase, at 733,211 to 734,032
GFF3007
GFF3008 - hypothetical protein, at 734,116 to 734,817
GFF3008
Position (kb) 732
733
734 Strain fitness (log2 ratio) -2
-1
0
1 at 732.560 kb on - strand, within GFF3005 at 732.813 kb on - strand at 733.051 kb on - strand, within GFF3006 at 733.051 kb on - strand, within GFF3006 at 733.059 kb on + strand, within GFF3006 at 733.059 kb on + strand, within GFF3006 at 733.059 kb on + strand, within GFF3006 at 733.059 kb on + strand, within GFF3006 at 733.060 kb on - strand, within GFF3006 at 733.060 kb on - strand, within GFF3006 at 733.095 kb on + strand, within GFF3006 at 733.096 kb on - strand, within GFF3006 at 733.096 kb on - strand, within GFF3006 at 733.537 kb on - strand, within GFF3007 at 733.935 kb on + strand, within GFF3007 at 733.936 kb on - strand, within GFF3007 at 734.087 kb on - strand at 734.105 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Methanol and casaminos (C) remove 732,560 - GFF3005 0.79 +0.9 732,813 - -0.5 733,051 - GFF3006 0.63 +0.1 733,051 - GFF3006 0.63 +0.4 733,059 + GFF3006 0.65 -1.1 733,059 + GFF3006 0.65 +0.7 733,059 + GFF3006 0.65 -0.2 733,059 + GFF3006 0.65 -0.3 733,060 - GFF3006 0.65 -1.9 733,060 - GFF3006 0.65 -0.2 733,095 + GFF3006 0.73 -0.9 733,096 - GFF3006 0.73 -0.1 733,096 - GFF3006 0.73 -0.0 733,537 - GFF3007 0.40 +0.8 733,935 + GFF3007 0.88 +0.5 733,936 - GFF3007 0.88 +0.1 734,087 - +0.3 734,105 - +0.7
Or see this region's nucleotide sequence