Experiment: Methanol and casaminos (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF291 and GFF292 are separated by 79 nucleotides GFF292 and GFF293 are separated by 72 nucleotides GFF293 and GFF294 overlap by 4 nucleotides
GFF291 - Glutamine synthetase, at 305,838 to 307,193
GFF291
GFF292 - Isopentenyl-diphosphate delta-isomerase, at 307,273 to 308,610
GFF292
GFF293 - hypothetical protein, at 308,683 to 309,390
GFF293
GFF294 - Amidophosphoribosyltransferase, at 309,387 to 310,286
GFF294
Position (kb) 307
308
309 Strain fitness (log2 ratio) -2
-1
0
1
2 at 306.280 kb on + strand, within GFF291 at 306.423 kb on + strand, within GFF291 at 306.423 kb on + strand, within GFF291 at 306.423 kb on + strand, within GFF291 at 306.423 kb on + strand, within GFF291 at 306.423 kb on + strand, within GFF291 at 306.423 kb on + strand, within GFF291 at 306.423 kb on + strand, within GFF291 at 306.424 kb on - strand, within GFF291 at 306.424 kb on - strand, within GFF291 at 306.424 kb on - strand, within GFF291 at 306.424 kb on - strand, within GFF291 at 306.424 kb on - strand, within GFF291 at 306.424 kb on - strand, within GFF291 at 306.520 kb on + strand, within GFF291 at 306.521 kb on - strand, within GFF291 at 306.565 kb on + strand, within GFF291 at 306.566 kb on - strand, within GFF291 at 306.697 kb on + strand, within GFF291 at 306.698 kb on - strand, within GFF291 at 306.763 kb on + strand, within GFF291 at 306.764 kb on - strand, within GFF291 at 306.793 kb on + strand, within GFF291 at 306.871 kb on + strand, within GFF291 at 306.872 kb on - strand, within GFF291 at 306.872 kb on - strand, within GFF291 at 306.872 kb on - strand, within GFF291 at 306.872 kb on - strand, within GFF291 at 307.078 kb on + strand at 307.078 kb on + strand at 307.079 kb on - strand at 307.094 kb on - strand at 307.263 kb on + strand at 307.263 kb on + strand at 307.264 kb on - strand at 307.277 kb on + strand at 307.445 kb on + strand, within GFF292 at 307.445 kb on + strand, within GFF292 at 307.445 kb on + strand, within GFF292 at 307.445 kb on + strand, within GFF292 at 307.580 kb on + strand, within GFF292 at 307.889 kb on + strand, within GFF292 at 307.889 kb on + strand, within GFF292 at 307.890 kb on - strand, within GFF292 at 307.893 kb on - strand, within GFF292 at 307.919 kb on + strand, within GFF292 at 307.919 kb on + strand, within GFF292 at 307.919 kb on + strand, within GFF292 at 308.168 kb on + strand, within GFF292 at 308.168 kb on + strand, within GFF292 at 308.168 kb on + strand, within GFF292 at 308.169 kb on - strand, within GFF292 at 308.190 kb on - strand, within GFF292 at 308.507 kb on + strand at 308.507 kb on + strand at 308.507 kb on + strand at 308.508 kb on - strand at 308.508 kb on - strand at 308.508 kb on - strand at 308.610 kb on + strand at 308.610 kb on + strand at 308.610 kb on + strand at 308.610 kb on + strand at 308.611 kb on - strand at 308.891 kb on + strand, within GFF293 at 309.194 kb on + strand, within GFF293 at 309.195 kb on - strand, within GFF293 at 309.195 kb on - strand, within GFF293 at 309.197 kb on + strand, within GFF293 at 309.197 kb on + strand, within GFF293 at 309.197 kb on + strand, within GFF293 at 309.197 kb on + strand, within GFF293 at 309.409 kb on + strand at 309.478 kb on + strand, within GFF294 at 309.499 kb on + strand, within GFF294 at 309.500 kb on - strand, within GFF294
Per-strain Table
Position Strand Gene LocusTag Fraction Methanol and casaminos (C) remove 306,280 + GFF291 0.33 +0.5 306,423 + GFF291 0.43 +1.1 306,423 + GFF291 0.43 -0.5 306,423 + GFF291 0.43 -0.4 306,423 + GFF291 0.43 +0.3 306,423 + GFF291 0.43 +0.1 306,423 + GFF291 0.43 -0.4 306,423 + GFF291 0.43 -0.2 306,424 - GFF291 0.43 +2.0 306,424 - GFF291 0.43 -0.4 306,424 - GFF291 0.43 -0.2 306,424 - GFF291 0.43 -0.7 306,424 - GFF291 0.43 -0.4 306,424 - GFF291 0.43 +0.6 306,520 + GFF291 0.50 +0.4 306,521 - GFF291 0.50 +0.2 306,565 + GFF291 0.54 +1.0 306,566 - GFF291 0.54 +0.6 306,697 + GFF291 0.63 -0.3 306,698 - GFF291 0.63 -0.4 306,763 + GFF291 0.68 +0.2 306,764 - GFF291 0.68 -0.2 306,793 + GFF291 0.70 +1.4 306,871 + GFF291 0.76 +0.4 306,872 - GFF291 0.76 -0.0 306,872 - GFF291 0.76 +1.3 306,872 - GFF291 0.76 +1.1 306,872 - GFF291 0.76 -0.1 307,078 + -0.1 307,078 + +0.4 307,079 - +0.4 307,094 - +0.3 307,263 + -0.5 307,263 + +1.4 307,264 - -0.5 307,277 + -0.1 307,445 + GFF292 0.13 +0.2 307,445 + GFF292 0.13 +0.9 307,445 + GFF292 0.13 -0.2 307,445 + GFF292 0.13 -0.3 307,580 + GFF292 0.23 -0.3 307,889 + GFF292 0.46 -0.2 307,889 + GFF292 0.46 +0.6 307,890 - GFF292 0.46 -1.2 307,893 - GFF292 0.46 +0.1 307,919 + GFF292 0.48 -0.5 307,919 + GFF292 0.48 -1.2 307,919 + GFF292 0.48 -0.5 308,168 + GFF292 0.67 +0.7 308,168 + GFF292 0.67 -1.0 308,168 + GFF292 0.67 -0.3 308,169 - GFF292 0.67 +0.9 308,190 - GFF292 0.69 -1.9 308,507 + -1.3 308,507 + +0.3 308,507 + +0.1 308,508 - +0.2 308,508 - +1.4 308,508 - -0.1 308,610 + -0.2 308,610 + -0.0 308,610 + +0.2 308,610 + -0.2 308,611 - -0.2 308,891 + GFF293 0.29 -0.1 309,194 + GFF293 0.72 -0.5 309,195 - GFF293 0.72 -0.8 309,195 - GFF293 0.72 -0.3 309,197 + GFF293 0.73 +0.0 309,197 + GFF293 0.73 -0.0 309,197 + GFF293 0.73 -0.0 309,197 + GFF293 0.73 +0.6 309,409 + -1.7 309,478 + GFF294 0.10 -0.4 309,499 + GFF294 0.12 -0.6 309,500 - GFF294 0.13 +0.2
Or see this region's nucleotide sequence