Experiment: Methanol and casaminos (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF261 and GFF262 are separated by 197 nucleotides GFF262 and GFF263 are separated by 63 nucleotides GFF263 and GFF264 are separated by 202 nucleotides GFF264 and GFF265 are separated by 401 nucleotides
GFF261 - Alpha-keto acid-binding periplasmic protein TakP, at 276,205 to 277,299
GFF261
GFF262 - hypothetical protein, at 277,497 to 278,051
GFF262
GFF263 - hypothetical protein, at 278,115 to 278,846
GFF263
GFF264 - hypothetical protein, at 279,049 to 279,366
GFF264
GFF265 - Cysteine--tRNA ligase, at 279,768 to 281,156
GFF265
Position (kb) 278
279 Strain fitness (log2 ratio) -2
-1
0
1 at 277.127 kb on + strand, within GFF261 at 277.127 kb on + strand, within GFF261 at 277.128 kb on - strand, within GFF261 at 277.128 kb on - strand, within GFF261 at 277.331 kb on + strand at 277.332 kb on - strand at 277.332 kb on - strand at 277.332 kb on - strand at 277.420 kb on - strand at 277.420 kb on - strand at 277.456 kb on + strand at 277.457 kb on - strand at 277.457 kb on - strand at 277.457 kb on - strand at 277.722 kb on + strand, within GFF262 at 277.723 kb on - strand, within GFF262 at 277.933 kb on - strand, within GFF262 at 278.016 kb on - strand at 278.149 kb on + strand at 278.150 kb on - strand at 278.297 kb on - strand, within GFF263 at 278.297 kb on - strand, within GFF263 at 278.297 kb on - strand, within GFF263 at 278.620 kb on + strand, within GFF263 at 278.620 kb on + strand, within GFF263 at 278.620 kb on + strand, within GFF263 at 278.620 kb on + strand, within GFF263 at 278.621 kb on - strand, within GFF263 at 278.651 kb on - strand, within GFF263 at 278.651 kb on - strand, within GFF263 at 278.717 kb on - strand, within GFF263 at 278.931 kb on - strand at 278.931 kb on - strand at 278.931 kb on - strand at 279.168 kb on - strand, within GFF264 at 279.182 kb on + strand, within GFF264 at 279.182 kb on + strand, within GFF264 at 279.388 kb on - strand at 279.388 kb on - strand at 279.388 kb on - strand at 279.519 kb on + strand at 279.519 kb on + strand at 279.520 kb on - strand at 279.644 kb on + strand at 279.695 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Methanol and casaminos (C) remove 277,127 + GFF261 0.84 +1.0 277,127 + GFF261 0.84 +0.7 277,128 - GFF261 0.84 -0.3 277,128 - GFF261 0.84 -0.1 277,331 + +0.4 277,332 - -0.9 277,332 - +0.1 277,332 - +0.0 277,420 - -2.1 277,420 - -0.1 277,456 + +0.2 277,457 - -0.2 277,457 - +0.1 277,457 - -0.1 277,722 + GFF262 0.41 +0.0 277,723 - GFF262 0.41 -0.3 277,933 - GFF262 0.79 +0.5 278,016 - -0.5 278,149 + +1.1 278,150 - +0.7 278,297 - GFF263 0.25 -0.3 278,297 - GFF263 0.25 -0.9 278,297 - GFF263 0.25 +0.3 278,620 + GFF263 0.69 +0.1 278,620 + GFF263 0.69 +0.1 278,620 + GFF263 0.69 +0.3 278,620 + GFF263 0.69 +0.0 278,621 - GFF263 0.69 +1.2 278,651 - GFF263 0.73 -0.8 278,651 - GFF263 0.73 +0.7 278,717 - GFF263 0.82 -0.3 278,931 - -0.0 278,931 - +0.7 278,931 - -0.5 279,168 - GFF264 0.37 -1.4 279,182 + GFF264 0.42 -0.1 279,182 + GFF264 0.42 +0.3 279,388 - -0.5 279,388 - -0.0 279,388 - +0.0 279,519 + +0.5 279,519 + +0.0 279,520 - +0.1 279,644 + -0.2 279,695 + -0.2
Or see this region's nucleotide sequence