Experiment: Methanol and casaminos (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF186 and GFF187 are separated by 65 nucleotides GFF187 and GFF188 overlap by 4 nucleotides GFF188 and GFF189 overlap by 1 nucleotides GFF189 and GFF190 overlap by 1 nucleotides
GFF186 - hypothetical protein, at 195,587 to 196,750
GFF186
GFF187 - Ureidoglycolate lyase, at 196,816 to 197,289
GFF187
GFF188 - N-carbamoyl-L-amino-acid hydrolase, at 197,286 to 198,536
GFF188
GFF189 - Oligopeptide transport ATP-binding protein OppD, at 198,536 to 199,525
GFF189
GFF190 - Glutamyl-tRNA(Gln) amidotransferase subunit A, at 199,525 to 200,967
GFF190
Position (kb) 197
198
199 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 196.356 kb on - strand, within GFF186 at 196.356 kb on - strand, within GFF186 at 196.536 kb on - strand, within GFF186 at 196.818 kb on - strand at 197.086 kb on - strand, within GFF187 at 197.240 kb on + strand, within GFF187 at 197.525 kb on + strand, within GFF188 at 197.526 kb on - strand, within GFF188 at 197.575 kb on - strand, within GFF188 at 197.846 kb on + strand, within GFF188 at 197.846 kb on + strand, within GFF188 at 197.846 kb on + strand, within GFF188 at 197.847 kb on - strand, within GFF188 at 197.879 kb on - strand, within GFF188 at 198.097 kb on + strand, within GFF188 at 198.098 kb on - strand, within GFF188 at 198.098 kb on - strand, within GFF188 at 198.507 kb on - strand at 198.539 kb on - strand at 198.575 kb on + strand at 198.575 kb on + strand at 198.575 kb on + strand at 198.575 kb on + strand at 198.575 kb on + strand at 198.576 kb on - strand at 198.576 kb on - strand at 198.576 kb on - strand at 198.831 kb on + strand, within GFF189 at 198.831 kb on + strand, within GFF189 at 198.831 kb on + strand, within GFF189 at 198.831 kb on + strand, within GFF189 at 198.831 kb on + strand, within GFF189 at 198.832 kb on - strand, within GFF189 at 198.832 kb on - strand, within GFF189 at 198.832 kb on - strand, within GFF189 at 198.832 kb on - strand, within GFF189 at 198.832 kb on - strand, within GFF189 at 198.832 kb on - strand, within GFF189 at 198.832 kb on - strand, within GFF189 at 198.832 kb on - strand, within GFF189 at 199.065 kb on + strand, within GFF189 at 199.065 kb on + strand, within GFF189 at 199.197 kb on + strand, within GFF189 at 199.197 kb on + strand, within GFF189 at 199.198 kb on - strand, within GFF189 at 199.198 kb on - strand, within GFF189 at 199.198 kb on - strand, within GFF189 at 199.198 kb on - strand, within GFF189 at 199.198 kb on - strand, within GFF189
Per-strain Table
Position Strand Gene LocusTag Fraction Methanol and casaminos (C) remove 196,356 - GFF186 0.66 -0.9 196,356 - GFF186 0.66 +0.5 196,536 - GFF186 0.82 -3.0 196,818 - +0.5 197,086 - GFF187 0.57 -0.3 197,240 + GFF187 0.89 +0.1 197,525 + GFF188 0.19 -0.1 197,526 - GFF188 0.19 +0.1 197,575 - GFF188 0.23 +0.5 197,846 + GFF188 0.45 +0.1 197,846 + GFF188 0.45 -0.3 197,846 + GFF188 0.45 +1.1 197,847 - GFF188 0.45 -1.5 197,879 - GFF188 0.47 +0.0 198,097 + GFF188 0.65 -0.5 198,098 - GFF188 0.65 -1.8 198,098 - GFF188 0.65 -0.2 198,507 - +0.0 198,539 - -0.9 198,575 + -0.2 198,575 + +0.5 198,575 + -0.6 198,575 + +0.4 198,575 + +0.0 198,576 - -0.6 198,576 - -0.5 198,576 - +0.7 198,831 + GFF189 0.30 +0.3 198,831 + GFF189 0.30 +0.1 198,831 + GFF189 0.30 -0.1 198,831 + GFF189 0.30 -0.7 198,831 + GFF189 0.30 +0.3 198,832 - GFF189 0.30 -0.6 198,832 - GFF189 0.30 -0.7 198,832 - GFF189 0.30 -1.3 198,832 - GFF189 0.30 +0.2 198,832 - GFF189 0.30 -0.4 198,832 - GFF189 0.30 -0.7 198,832 - GFF189 0.30 -0.1 198,832 - GFF189 0.30 -0.8 199,065 + GFF189 0.53 +0.3 199,065 + GFF189 0.53 -0.1 199,197 + GFF189 0.67 -0.9 199,197 + GFF189 0.67 +0.5 199,198 - GFF189 0.67 +0.4 199,198 - GFF189 0.67 +0.7 199,198 - GFF189 0.67 -0.8 199,198 - GFF189 0.67 -0.5 199,198 - GFF189 0.67 +0.2
Or see this region's nucleotide sequence