Experiment: Methanol and casaminos (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1852 and GFF1853 are separated by 457 nucleotides GFF1853 and GFF1854 are separated by 28 nucleotides
GFF1852 - 3-isopropylmalate dehydratase large subunit, at 512,566 to 513,990
GFF1852
GFF1853 - hypothetical protein, at 514,448 to 515,164
GFF1853
GFF1854 - Gamma-glutamylputrescine oxidoreductase, at 515,193 to 516,497
GFF1854
Position (kb) 514
515
516 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 513.680 kb on + strand, within GFF1852 at 513.895 kb on - strand at 513.897 kb on - strand at 513.897 kb on - strand at 513.897 kb on - strand at 513.897 kb on - strand at 513.897 kb on - strand at 513.956 kb on + strand at 514.060 kb on - strand at 514.074 kb on - strand at 514.074 kb on - strand at 514.373 kb on + strand at 514.373 kb on + strand at 514.374 kb on - strand at 514.577 kb on - strand, within GFF1853 at 514.715 kb on + strand, within GFF1853 at 514.913 kb on + strand, within GFF1853 at 514.914 kb on - strand, within GFF1853 at 514.914 kb on - strand, within GFF1853 at 514.994 kb on + strand, within GFF1853 at 515.162 kb on + strand at 515.246 kb on - strand at 515.246 kb on - strand at 515.643 kb on + strand, within GFF1854 at 515.643 kb on + strand, within GFF1854 at 515.644 kb on - strand, within GFF1854 at 515.644 kb on - strand, within GFF1854 at 515.764 kb on + strand, within GFF1854 at 515.955 kb on + strand, within GFF1854 at 515.955 kb on + strand, within GFF1854
Per-strain Table
Position Strand Gene LocusTag Fraction Methanol and casaminos (C) remove 513,680 + GFF1852 0.78 -0.8 513,895 - -2.4 513,897 - -1.8 513,897 - -2.0 513,897 - -1.7 513,897 - -3.4 513,897 - -1.8 513,956 + -1.5 514,060 - -0.9 514,074 - -0.8 514,074 - +0.7 514,373 + +0.2 514,373 + -0.5 514,374 - +0.6 514,577 - GFF1853 0.18 +0.0 514,715 + GFF1853 0.37 +0.0 514,913 + GFF1853 0.65 +0.1 514,914 - GFF1853 0.65 -0.1 514,914 - GFF1853 0.65 -0.6 514,994 + GFF1853 0.76 -0.2 515,162 + -0.1 515,246 - +0.4 515,246 - +0.7 515,643 + GFF1854 0.34 -0.3 515,643 + GFF1854 0.34 +0.2 515,644 - GFF1854 0.35 +0.6 515,644 - GFF1854 0.35 +0.2 515,764 + GFF1854 0.44 +0.1 515,955 + GFF1854 0.58 +0.7 515,955 + GFF1854 0.58 -0.0
Or see this region's nucleotide sequence