Experiment: Methanol and casaminos (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1840 and GFF1841 are separated by 251 nucleotides GFF1841 and GFF1842 are separated by 76 nucleotides GFF1842 and GFF1843 are separated by 188 nucleotides GFF1843 and GFF1844 are separated by 43 nucleotides
GFF1840 - hypothetical protein, at 502,135 to 502,254
GFF1840
GFF1841 - hypothetical protein, at 502,506 to 502,937
GFF1841
GFF1842 - Cytochrome b561, at 503,014 to 503,571
GFF1842
GFF1843 - 3-isopropylmalate dehydratase small subunit 1, at 503,760 to 504,365
GFF1843
GFF1844 - Protein-glutamate methylesterase/protein-glutamine glutaminase 1, at 504,409 to 505,065
GFF1844
Position (kb) 503
504 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 502.475 kb on + strand at 502.486 kb on + strand at 502.522 kb on + strand at 502.794 kb on + strand, within GFF1841 at 502.795 kb on - strand, within GFF1841 at 502.815 kb on + strand, within GFF1841 at 502.816 kb on - strand, within GFF1841 at 502.953 kb on + strand at 502.953 kb on + strand at 502.954 kb on - strand at 502.954 kb on - strand at 503.110 kb on + strand, within GFF1842 at 503.110 kb on + strand, within GFF1842 at 503.111 kb on - strand, within GFF1842 at 503.628 kb on + strand at 503.629 kb on - strand at 503.677 kb on + strand at 503.680 kb on + strand at 503.681 kb on - strand at 503.845 kb on - strand, within GFF1843 at 503.845 kb on - strand, within GFF1843 at 503.845 kb on - strand, within GFF1843 at 503.901 kb on + strand, within GFF1843 at 504.328 kb on - strand at 504.328 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Methanol and casaminos (C) remove 502,475 + -2.2 502,486 + -0.7 502,522 + -0.1 502,794 + GFF1841 0.67 -0.3 502,795 - GFF1841 0.67 -0.5 502,815 + GFF1841 0.72 -0.3 502,816 - GFF1841 0.72 -0.2 502,953 + +0.4 502,953 + +0.9 502,954 - +0.3 502,954 - -1.5 503,110 + GFF1842 0.17 +0.7 503,110 + GFF1842 0.17 -0.0 503,111 - GFF1842 0.17 -0.2 503,628 + -1.0 503,629 - -1.3 503,677 + -3.2 503,680 + -0.2 503,681 - -2.0 503,845 - GFF1843 0.14 -3.5 503,845 - GFF1843 0.14 -1.7 503,845 - GFF1843 0.14 -2.2 503,901 + GFF1843 0.23 -2.0 504,328 - -1.0 504,328 - -2.3
Or see this region's nucleotide sequence