Experiment: Methanol and casaminos (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF172 and GFF173 are separated by 80 nucleotides GFF173 and GFF174 are separated by 66 nucleotides GFF174 and GFF175 overlap by 11 nucleotides
GFF172 - Ketol-acid reductoisomerase (NADP(+)), at 181,317 to 182,336
GFF172
GFF173 - hypothetical protein, at 182,417 to 182,902
GFF173
GFF174 - 3-mercaptopropionate dioxygenase, at 182,969 to 183,616
GFF174
GFF175 - hypothetical protein, at 183,606 to 184,343
GFF175
Position (kb) 182
183
184 Strain fitness (log2 ratio) -1
0
1 at 182.391 kb on + strand at 182.625 kb on + strand, within GFF173 at 182.625 kb on + strand, within GFF173 at 182.625 kb on + strand, within GFF173 at 182.626 kb on - strand, within GFF173 at 182.626 kb on - strand, within GFF173 at 182.651 kb on + strand, within GFF173 at 182.652 kb on - strand, within GFF173 at 182.938 kb on + strand at 182.939 kb on - strand at 183.034 kb on - strand, within GFF174 at 183.387 kb on + strand, within GFF174 at 183.396 kb on + strand, within GFF174 at 183.951 kb on + strand, within GFF175 at 183.952 kb on - strand, within GFF175 at 184.070 kb on + strand, within GFF175 at 184.070 kb on + strand, within GFF175 at 184.226 kb on + strand, within GFF175 at 184.226 kb on + strand, within GFF175 at 184.226 kb on + strand, within GFF175 at 184.227 kb on - strand, within GFF175 at 184.255 kb on + strand, within GFF175
Per-strain Table
Position Strand Gene LocusTag Fraction Methanol and casaminos (C) remove 182,391 + -1.2 182,625 + GFF173 0.43 +0.3 182,625 + GFF173 0.43 -0.6 182,625 + GFF173 0.43 +0.5 182,626 - GFF173 0.43 -0.0 182,626 - GFF173 0.43 +0.4 182,651 + GFF173 0.48 -0.3 182,652 - GFF173 0.48 +0.1 182,938 + -0.0 182,939 - +0.7 183,034 - GFF174 0.10 -0.0 183,387 + GFF174 0.65 -0.5 183,396 + GFF174 0.66 +0.2 183,951 + GFF175 0.47 +0.4 183,952 - GFF175 0.47 +0.1 184,070 + GFF175 0.63 -0.1 184,070 + GFF175 0.63 +0.3 184,226 + GFF175 0.84 +0.3 184,226 + GFF175 0.84 -0.1 184,226 + GFF175 0.84 +0.2 184,227 - GFF175 0.84 -0.9 184,255 + GFF175 0.88 +0.0
Or see this region's nucleotide sequence