Experiment: Methanol and casaminos (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1730 and GFF1731 are separated by 5 nucleotides GFF1731 and GFF1732 overlap by 4 nucleotides GFF1732 and GFF1733 are separated by 61 nucleotides GFF1733 and GFF1734 are separated by 196 nucleotides
GFF1730 - Molybdopterin synthase catalytic subunit, at 378,672 to 379,142
GFF1730
GFF1731 - Molybdopterin synthase sulfur carrier subunit, at 379,148 to 379,399
GFF1731
GFF1732 - CDP-diacylglycerol--glycerol-3-phosphate 3-phosphatidyltransferase, at 379,396 to 380,076
GFF1732
GFF1733 - UvrABC system protein C, at 380,138 to 382,498
GFF1733
GFF1734 - hypothetical protein, at 382,695 to 383,300
GFF1734
Position (kb) 380
381
382
383 Strain fitness (log2 ratio) -1
0
1
2 at 379.266 kb on + strand, within GFF1731 at 380.052 kb on - strand at 380.096 kb on - strand at 380.096 kb on - strand at 380.096 kb on - strand at 380.100 kb on - strand at 380.164 kb on - strand at 380.170 kb on - strand at 380.396 kb on - strand, within GFF1733 at 380.397 kb on + strand, within GFF1733 at 380.397 kb on + strand, within GFF1733 at 380.398 kb on - strand, within GFF1733 at 380.398 kb on - strand, within GFF1733 at 380.398 kb on - strand, within GFF1733 at 380.401 kb on - strand, within GFF1733 at 380.971 kb on - strand, within GFF1733 at 381.051 kb on + strand, within GFF1733 at 381.388 kb on - strand, within GFF1733 at 381.388 kb on - strand, within GFF1733 at 381.388 kb on - strand, within GFF1733 at 381.391 kb on - strand, within GFF1733 at 381.601 kb on - strand, within GFF1733 at 381.676 kb on - strand, within GFF1733 at 381.762 kb on + strand, within GFF1733 at 381.762 kb on + strand, within GFF1733 at 381.762 kb on + strand, within GFF1733 at 381.762 kb on + strand, within GFF1733 at 381.763 kb on - strand, within GFF1733 at 381.763 kb on - strand, within GFF1733 at 381.763 kb on - strand, within GFF1733 at 381.850 kb on - strand, within GFF1733 at 381.852 kb on + strand, within GFF1733 at 381.852 kb on + strand, within GFF1733 at 381.927 kb on + strand, within GFF1733 at 381.927 kb on + strand, within GFF1733 at 381.928 kb on - strand, within GFF1733 at 382.176 kb on + strand, within GFF1733 at 382.176 kb on + strand, within GFF1733 at 382.422 kb on + strand at 382.423 kb on - strand at 382.426 kb on + strand at 382.427 kb on - strand at 382.490 kb on + strand at 382.491 kb on - strand at 382.491 kb on - strand at 382.567 kb on + strand at 382.571 kb on + strand at 382.571 kb on + strand at 382.572 kb on - strand at 382.572 kb on - strand at 382.572 kb on - strand at 382.576 kb on - strand at 382.576 kb on - strand at 382.578 kb on + strand at 382.579 kb on - strand at 382.827 kb on + strand, within GFF1734 at 382.827 kb on + strand, within GFF1734 at 382.827 kb on + strand, within GFF1734 at 382.912 kb on - strand, within GFF1734 at 382.989 kb on + strand, within GFF1734 at 383.055 kb on + strand, within GFF1734 at 383.076 kb on + strand, within GFF1734 at 383.077 kb on - strand, within GFF1734 at 383.158 kb on + strand, within GFF1734 at 383.267 kb on + strand at 383.310 kb on + strand at 383.368 kb on - strand at 383.480 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Methanol and casaminos (C) remove 379,266 + GFF1731 0.47 -0.5 380,052 - +0.2 380,096 - -0.1 380,096 - +0.3 380,096 - -0.5 380,100 - +0.6 380,164 - +0.2 380,170 - -0.3 380,396 - GFF1733 0.11 +0.9 380,397 + GFF1733 0.11 +0.5 380,397 + GFF1733 0.11 -0.1 380,398 - GFF1733 0.11 +0.3 380,398 - GFF1733 0.11 -0.6 380,398 - GFF1733 0.11 +0.2 380,401 - GFF1733 0.11 +0.7 380,971 - GFF1733 0.35 +0.8 381,051 + GFF1733 0.39 -1.0 381,388 - GFF1733 0.53 +0.7 381,388 - GFF1733 0.53 -0.4 381,388 - GFF1733 0.53 -0.7 381,391 - GFF1733 0.53 +0.3 381,601 - GFF1733 0.62 -0.1 381,676 - GFF1733 0.65 -0.8 381,762 + GFF1733 0.69 +0.3 381,762 + GFF1733 0.69 -0.0 381,762 + GFF1733 0.69 +1.2 381,762 + GFF1733 0.69 -0.2 381,763 - GFF1733 0.69 -0.1 381,763 - GFF1733 0.69 -0.0 381,763 - GFF1733 0.69 +1.0 381,850 - GFF1733 0.73 +0.6 381,852 + GFF1733 0.73 -1.3 381,852 + GFF1733 0.73 -0.1 381,927 + GFF1733 0.76 -0.8 381,927 + GFF1733 0.76 +0.3 381,928 - GFF1733 0.76 -1.0 382,176 + GFF1733 0.86 +0.2 382,176 + GFF1733 0.86 -0.7 382,422 + +0.9 382,423 - -0.5 382,426 + +1.1 382,427 - -1.0 382,490 + -1.1 382,491 - -1.1 382,491 - -0.8 382,567 + +1.1 382,571 + +0.3 382,571 + +0.5 382,572 - +1.8 382,572 - -0.2 382,572 - -0.8 382,576 - +0.4 382,576 - +0.8 382,578 + -0.1 382,579 - -0.3 382,827 + GFF1734 0.22 +0.6 382,827 + GFF1734 0.22 -0.5 382,827 + GFF1734 0.22 +0.9 382,912 - GFF1734 0.36 -0.1 382,989 + GFF1734 0.49 -0.3 383,055 + GFF1734 0.59 +0.7 383,076 + GFF1734 0.63 +0.3 383,077 - GFF1734 0.63 +0.6 383,158 + GFF1734 0.76 +0.2 383,267 + +0.6 383,310 + +0.8 383,368 - -1.1 383,480 + -0.3
Or see this region's nucleotide sequence