Experiment: Methanol and casaminos (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1673 and GFF1674 are separated by 82 nucleotides GFF1674 and GFF1675 are separated by 3 nucleotides GFF1675 and GFF1676 are separated by 177 nucleotides GFF1676 and GFF1677 are separated by 78 nucleotides
GFF1673 - Putative alcohol dehydrogenase D, at 327,992 to 329,119
GFF1673
GFF1674 - hypothetical protein, at 329,202 to 329,339
GFF1674
GFF1675 - Thiosulfate dehydrogenase, at 329,343 to 330,404
GFF1675
GFF1676 - 50S ribosomal protein L25, at 330,582 to 331,184
GFF1676
GFF1677 - Peptidyl-tRNA hydrolase, at 331,263 to 331,871
GFF1677
Position (kb)
329
330
331 Strain fitness (log2 ratio)
-1
0
1 at 328.412 kb on + strand, within GFF1673 at 328.412 kb on + strand, within GFF1673 at 328.412 kb on + strand, within GFF1673 at 328.412 kb on + strand, within GFF1673 at 328.412 kb on + strand, within GFF1673 at 328.412 kb on + strand, within GFF1673 at 328.413 kb on - strand, within GFF1673 at 328.413 kb on - strand, within GFF1673 at 328.445 kb on + strand, within GFF1673 at 328.445 kb on + strand, within GFF1673 at 328.446 kb on - strand, within GFF1673 at 328.830 kb on - strand, within GFF1673 at 328.830 kb on - strand, within GFF1673 at 328.946 kb on + strand, within GFF1673 at 328.946 kb on + strand, within GFF1673 at 328.946 kb on + strand, within GFF1673 at 328.946 kb on + strand, within GFF1673 at 328.946 kb on + strand, within GFF1673 at 328.946 kb on + strand, within GFF1673 at 328.946 kb on + strand, within GFF1673 at 328.947 kb on - strand, within GFF1673 at 328.947 kb on - strand, within GFF1673 at 328.947 kb on - strand, within GFF1673 at 328.947 kb on - strand, within GFF1673 at 328.947 kb on - strand, within GFF1673 at 328.998 kb on - strand, within GFF1673 at 328.998 kb on - strand, within GFF1673 at 329.114 kb on + strand at 329.115 kb on - strand at 329.337 kb on + strand at 329.337 kb on + strand at 329.425 kb on + strand at 329.426 kb on - strand at 329.426 kb on - strand at 329.551 kb on + strand, within GFF1675 at 329.551 kb on + strand, within GFF1675 at 329.551 kb on + strand, within GFF1675 at 329.788 kb on + strand, within GFF1675 at 329.788 kb on + strand, within GFF1675 at 329.902 kb on + strand, within GFF1675 at 329.903 kb on - strand, within GFF1675 at 330.031 kb on + strand, within GFF1675 at 330.031 kb on + strand, within GFF1675 at 330.190 kb on + strand, within GFF1675 at 330.191 kb on - strand, within GFF1675 at 330.209 kb on - strand, within GFF1675 at 330.209 kb on - strand, within GFF1675 at 330.209 kb on - strand, within GFF1675 at 330.209 kb on - strand, within GFF1675 at 330.560 kb on + strand at 330.560 kb on + strand at 330.563 kb on + strand at 330.563 kb on + strand at 330.563 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Methanol and casaminos (C) remove 328,412 + GFF1673 0.37 +1.1 328,412 + GFF1673 0.37 +0.1 328,412 + GFF1673 0.37 -1.4 328,412 + GFF1673 0.37 -0.1 328,412 + GFF1673 0.37 -0.3 328,412 + GFF1673 0.37 +0.3 328,413 - GFF1673 0.37 -0.6 328,413 - GFF1673 0.37 -0.2 328,445 + GFF1673 0.40 +0.3 328,445 + GFF1673 0.40 -0.8 328,446 - GFF1673 0.40 -0.9 328,830 - GFF1673 0.74 -1.1 328,830 - GFF1673 0.74 +0.9 328,946 + GFF1673 0.85 +0.1 328,946 + GFF1673 0.85 +0.7 328,946 + GFF1673 0.85 -0.7 328,946 + GFF1673 0.85 -1.5 328,946 + GFF1673 0.85 -0.2 328,946 + GFF1673 0.85 +0.5 328,946 + GFF1673 0.85 -0.1 328,947 - GFF1673 0.85 +0.1 328,947 - GFF1673 0.85 +0.7 328,947 - GFF1673 0.85 -0.9 328,947 - GFF1673 0.85 -0.1 328,947 - GFF1673 0.85 +0.6 328,998 - GFF1673 0.89 +0.4 328,998 - GFF1673 0.89 -1.0 329,114 + -1.1 329,115 - +0.3 329,337 + -0.5 329,337 + +1.7 329,425 + -0.1 329,426 - +0.2 329,426 - -0.1 329,551 + GFF1675 0.20 +0.2 329,551 + GFF1675 0.20 +0.1 329,551 + GFF1675 0.20 -1.1 329,788 + GFF1675 0.42 -0.5 329,788 + GFF1675 0.42 +0.4 329,902 + GFF1675 0.53 +0.2 329,903 - GFF1675 0.53 +0.4 330,031 + GFF1675 0.65 -1.2 330,031 + GFF1675 0.65 +0.1 330,190 + GFF1675 0.80 -1.2 330,191 - GFF1675 0.80 -0.5 330,209 - GFF1675 0.82 +0.2 330,209 - GFF1675 0.82 +1.1 330,209 - GFF1675 0.82 -0.2 330,209 - GFF1675 0.82 +0.0 330,560 + +0.7 330,560 + +0.1 330,563 + -0.6 330,563 + -0.6 330,563 + -0.2
Or see this region's nucleotide sequence