Experiment: LB_noSalt with Chloride 200 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt QEN71_RS35235 and QEN71_RS35240 are separated by 80 nucleotides QEN71_RS35240 and QEN71_RS35245 are separated by 535 nucleotides QEN71_RS35245 and QEN71_RS35250 are separated by 9 nucleotides QEN71_RS35250 and QEN71_RS35255 overlap by 2 nucleotides
QEN71_RS35235: QEN71_RS35235 - hypothetical protein, at 1,255,150 to 1,256,814
_RS35235
QEN71_RS35240: QEN71_RS35240 - IS5/IS1182 family transposase, at 1,256,895 to 1,256,993
_RS35240
QEN71_RS35245: QEN71_RS35245 - hypothetical protein, at 1,257,529 to 1,258,047
_RS35245
QEN71_RS35250: QEN71_RS35250 - hypothetical protein, at 1,258,057 to 1,258,248
_RS35250
QEN71_RS35255: QEN71_RS35255 - tetratricopeptide repeat protein, at 1,258,247 to 1,259,734
_RS35255
Position (kb) 1257
1258
1259 Strain fitness (log2 ratio) -2
-1
0
1 at 1256.616 kb on + strand, within QEN71_RS35235 at 1256.616 kb on + strand, within QEN71_RS35235 at 1256.617 kb on - strand, within QEN71_RS35235 at 1256.617 kb on - strand, within QEN71_RS35235 at 1256.682 kb on + strand at 1256.692 kb on + strand at 1256.693 kb on - strand at 1256.929 kb on - strand, within QEN71_RS35240 at 1256.929 kb on - strand, within QEN71_RS35240 at 1257.012 kb on - strand at 1257.541 kb on - strand at 1257.556 kb on + strand at 1257.680 kb on + strand, within QEN71_RS35245 at 1257.894 kb on - strand, within QEN71_RS35245 at 1257.894 kb on - strand, within QEN71_RS35245 at 1258.065 kb on + strand at 1258.235 kb on - strand at 1258.235 kb on - strand at 1258.265 kb on + strand at 1258.265 kb on + strand at 1258.266 kb on - strand at 1258.372 kb on - strand at 1258.537 kb on - strand, within QEN71_RS35255 at 1258.543 kb on - strand, within QEN71_RS35255 at 1259.005 kb on - strand, within QEN71_RS35255
Per-strain Table
Position Strand Gene LocusTag Fraction LB_noSalt with Chloride 200 mM remove 1,256,616 + QEN71_RS35235 0.88 +0.1 1,256,616 + QEN71_RS35235 0.88 -0.9 1,256,617 - QEN71_RS35235 0.88 +0.9 1,256,617 - QEN71_RS35235 0.88 +0.4 1,256,682 + -0.7 1,256,692 + +0.6 1,256,693 - +0.9 1,256,929 - QEN71_RS35240 0.34 -0.4 1,256,929 - QEN71_RS35240 0.34 +0.0 1,257,012 - +0.4 1,257,541 - -1.0 1,257,556 + +0.2 1,257,680 + QEN71_RS35245 0.29 +1.2 1,257,894 - QEN71_RS35245 0.70 +0.5 1,257,894 - QEN71_RS35245 0.70 +0.1 1,258,065 + +0.3 1,258,235 - +0.0 1,258,235 - +0.3 1,258,265 + -1.2 1,258,265 + +0.1 1,258,266 - -1.4 1,258,372 - -1.8 1,258,537 - QEN71_RS35255 0.19 -1.0 1,258,543 - QEN71_RS35255 0.20 +0.5 1,259,005 - QEN71_RS35255 0.51 -0.4
Or see this region's nucleotide sequence