Experiment: biofilm; Day1; NLDM; Aluminum chloride 1mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt LRK53_RS05425 and LRK53_RS05430 overlap by 1 nucleotides LRK53_RS05430 and LRK53_RS05435 overlap by 1 nucleotides LRK53_RS05435 and LRK53_RS05440 are separated by 20 nucleotides LRK53_RS05440 and LRK53_RS05445 overlap by 4 nucleotides
LRK53_RS05425: LRK53_RS05425 - 6,7-dimethyl-8-ribityllumazine synthase, at 1,265,688 to 1,266,149
_RS05425
LRK53_RS05430: LRK53_RS05430 - transcription antitermination factor NusB, at 1,266,149 to 1,266,595
_RS05430
LRK53_RS05435: LRK53_RS05435 - GNAT family N-acetyltransferase, at 1,266,595 to 1,267,137
_RS05435
LRK53_RS05440: LRK53_RS05440 - thiamine-phosphate kinase, at 1,267,158 to 1,268,126
_RS05440
LRK53_RS05445: LRK53_RS05445 - phosphatidylglycerophosphatase A, at 1,268,123 to 1,268,647
_RS05445
Position (kb)
1266
1267
1268 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1265.640 kb on + strand at 1265.656 kb on + strand at 1265.656 kb on + strand at 1266.847 kb on + strand, within LRK53_RS05435 at 1266.847 kb on + strand, within LRK53_RS05435 at 1266.847 kb on + strand, within LRK53_RS05435 at 1266.847 kb on + strand, within LRK53_RS05435 at 1267.102 kb on + strand at 1267.102 kb on + strand at 1267.102 kb on + strand at 1267.102 kb on + strand at 1267.102 kb on + strand at 1267.102 kb on + strand at 1267.102 kb on + strand at 1267.102 kb on + strand at 1267.103 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction biofilm; Day1; NLDM; Aluminum chloride 1mM remove 1,265,640 + +0.4 1,265,656 + +2.5 1,265,656 + +1.0 1,266,847 + LRK53_RS05435 0.46 -1.8 1,266,847 + LRK53_RS05435 0.46 +0.4 1,266,847 + LRK53_RS05435 0.46 -1.3 1,266,847 + LRK53_RS05435 0.46 -1.1 1,267,102 + -1.4 1,267,102 + +0.1 1,267,102 + +0.6 1,267,102 + -1.8 1,267,102 + -0.1 1,267,102 + +0.3 1,267,102 + -2.2 1,267,102 + -1.6 1,267,103 - +0.2
Or see this region's nucleotide sequence