Experiment: LB_noSalt with Chloride 200 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt QEN71_RS42180 and QEN71_RS42185 overlap by 10 nucleotides QEN71_RS42185 and QEN71_RS42190 are separated by 56 nucleotides QEN71_RS42190 and QEN71_RS42195 are separated by 71 nucleotides QEN71_RS42195 and QEN71_RS42200 overlap by 4 nucleotides
QEN71_RS42180: QEN71_RS42180 - nitrogenase iron-molybdenum cofactor biosynthesis protein NifE, at 519,401 to 520,933
_RS42180
QEN71_RS42185: QEN71_RS42185 - nitrogenase iron-molybdenum cofactor biosynthesis protein NifN, at 520,924 to 522,273
_RS42185
QEN71_RS42190: QEN71_RS42190 - nitrogen fixation protein NifX, at 522,330 to 522,746
_RS42190
QEN71_RS42195: QEN71_RS42195 - CCE_0567 family metalloprotein, at 522,818 to 523,039
_RS42195
QEN71_RS42200: QEN71_RS42200 - ferredoxin III, nif-specific, at 523,036 to 523,338
_RS42200
Position (kb) 520
521
522
523 Strain fitness (log2 ratio) -2
-1
0
1 at 519.967 kb on + strand, within QEN71_RS42180 at 519.967 kb on + strand, within QEN71_RS42180 at 519.985 kb on + strand, within QEN71_RS42180 at 519.985 kb on + strand, within QEN71_RS42180 at 519.986 kb on - strand, within QEN71_RS42180 at 519.986 kb on - strand, within QEN71_RS42180 at 519.986 kb on - strand, within QEN71_RS42180 at 519.986 kb on - strand, within QEN71_RS42180 at 519.988 kb on + strand, within QEN71_RS42180 at 519.988 kb on + strand, within QEN71_RS42180 at 519.988 kb on + strand, within QEN71_RS42180 at 520.002 kb on - strand, within QEN71_RS42180 at 520.003 kb on + strand, within QEN71_RS42180 at 520.003 kb on + strand, within QEN71_RS42180 at 520.004 kb on - strand, within QEN71_RS42180 at 520.060 kb on + strand, within QEN71_RS42180 at 520.060 kb on + strand, within QEN71_RS42180 at 520.061 kb on - strand, within QEN71_RS42180 at 520.061 kb on - strand, within QEN71_RS42180 at 520.061 kb on - strand, within QEN71_RS42180 at 520.062 kb on + strand, within QEN71_RS42180 at 520.062 kb on + strand, within QEN71_RS42180 at 520.062 kb on + strand, within QEN71_RS42180 at 520.063 kb on - strand, within QEN71_RS42180 at 520.063 kb on - strand, within QEN71_RS42180 at 520.063 kb on - strand, within QEN71_RS42180 at 520.092 kb on - strand, within QEN71_RS42180 at 520.130 kb on - strand, within QEN71_RS42180 at 520.130 kb on - strand, within QEN71_RS42180 at 520.130 kb on - strand, within QEN71_RS42180 at 520.197 kb on - strand, within QEN71_RS42180 at 520.197 kb on - strand, within QEN71_RS42180 at 520.469 kb on - strand, within QEN71_RS42180 at 520.540 kb on + strand, within QEN71_RS42180 at 520.541 kb on - strand, within QEN71_RS42180 at 520.559 kb on + strand, within QEN71_RS42180 at 520.559 kb on + strand, within QEN71_RS42180 at 520.559 kb on + strand, within QEN71_RS42180 at 520.559 kb on + strand, within QEN71_RS42180 at 520.559 kb on + strand, within QEN71_RS42180 at 520.559 kb on + strand, within QEN71_RS42180 at 520.560 kb on - strand, within QEN71_RS42180 at 520.616 kb on + strand, within QEN71_RS42180 at 520.616 kb on + strand, within QEN71_RS42180 at 520.616 kb on + strand, within QEN71_RS42180 at 520.617 kb on - strand, within QEN71_RS42180 at 520.617 kb on - strand, within QEN71_RS42180 at 520.617 kb on - strand, within QEN71_RS42180 at 520.617 kb on - strand, within QEN71_RS42180 at 520.756 kb on + strand, within QEN71_RS42180 at 520.756 kb on + strand, within QEN71_RS42180 at 520.757 kb on - strand, within QEN71_RS42180 at 520.931 kb on + strand at 520.999 kb on + strand at 520.999 kb on + strand at 521.133 kb on + strand, within QEN71_RS42185 at 521.133 kb on + strand, within QEN71_RS42185 at 521.133 kb on + strand, within QEN71_RS42185 at 521.134 kb on - strand, within QEN71_RS42185 at 521.134 kb on - strand, within QEN71_RS42185 at 521.139 kb on + strand, within QEN71_RS42185 at 521.178 kb on - strand, within QEN71_RS42185 at 521.178 kb on - strand, within QEN71_RS42185 at 521.188 kb on - strand, within QEN71_RS42185 at 521.318 kb on - strand, within QEN71_RS42185 at 521.336 kb on - strand, within QEN71_RS42185 at 521.336 kb on - strand, within QEN71_RS42185 at 521.336 kb on - strand, within QEN71_RS42185 at 521.410 kb on - strand, within QEN71_RS42185 at 521.410 kb on - strand, within QEN71_RS42185 at 521.414 kb on + strand, within QEN71_RS42185 at 521.415 kb on - strand, within QEN71_RS42185 at 521.450 kb on + strand, within QEN71_RS42185 at 521.451 kb on - strand, within QEN71_RS42185 at 521.451 kb on - strand, within QEN71_RS42185 at 521.451 kb on - strand, within QEN71_RS42185 at 521.451 kb on - strand, within QEN71_RS42185 at 521.586 kb on + strand, within QEN71_RS42185 at 521.586 kb on + strand, within QEN71_RS42185 at 521.587 kb on - strand, within QEN71_RS42185 at 521.587 kb on - strand, within QEN71_RS42185 at 521.626 kb on - strand, within QEN71_RS42185 at 521.786 kb on - strand, within QEN71_RS42185 at 521.837 kb on - strand, within QEN71_RS42185 at 521.966 kb on + strand, within QEN71_RS42185 at 522.042 kb on + strand, within QEN71_RS42185 at 522.172 kb on - strand at 522.172 kb on - strand at 522.209 kb on + strand at 522.209 kb on + strand at 522.209 kb on + strand at 522.209 kb on + strand at 522.209 kb on + strand at 522.210 kb on - strand at 522.210 kb on - strand at 522.293 kb on - strand at 522.401 kb on + strand, within QEN71_RS42190 at 522.406 kb on - strand, within QEN71_RS42190 at 522.406 kb on - strand, within QEN71_RS42190 at 522.412 kb on + strand, within QEN71_RS42190 at 522.412 kb on + strand, within QEN71_RS42190 at 522.412 kb on + strand, within QEN71_RS42190 at 522.413 kb on - strand, within QEN71_RS42190 at 522.428 kb on + strand, within QEN71_RS42190 at 522.428 kb on + strand, within QEN71_RS42190 at 522.428 kb on + strand, within QEN71_RS42190 at 522.428 kb on + strand, within QEN71_RS42190 at 522.428 kb on + strand, within QEN71_RS42190 at 522.428 kb on + strand, within QEN71_RS42190 at 522.429 kb on - strand, within QEN71_RS42190 at 522.429 kb on - strand, within QEN71_RS42190 at 522.429 kb on - strand, within QEN71_RS42190 at 522.429 kb on - strand, within QEN71_RS42190 at 522.531 kb on + strand, within QEN71_RS42190 at 522.709 kb on - strand at 522.709 kb on - strand at 522.713 kb on + strand at 522.714 kb on - strand at 522.733 kb on + strand at 522.733 kb on + strand at 522.734 kb on - strand at 522.734 kb on - strand at 523.061 kb on + strand at 523.178 kb on + strand, within QEN71_RS42200
Per-strain Table
Position Strand Gene LocusTag Fraction LB_noSalt with Chloride 200 mM remove 519,967 + QEN71_RS42180 0.37 -0.8 519,967 + QEN71_RS42180 0.37 +0.5 519,985 + QEN71_RS42180 0.38 +0.4 519,985 + QEN71_RS42180 0.38 -0.4 519,986 - QEN71_RS42180 0.38 -0.2 519,986 - QEN71_RS42180 0.38 -2.2 519,986 - QEN71_RS42180 0.38 -0.7 519,986 - QEN71_RS42180 0.38 -0.0 519,988 + QEN71_RS42180 0.38 -2.5 519,988 + QEN71_RS42180 0.38 -0.2 519,988 + QEN71_RS42180 0.38 -1.0 520,002 - QEN71_RS42180 0.39 +0.3 520,003 + QEN71_RS42180 0.39 -1.2 520,003 + QEN71_RS42180 0.39 -1.0 520,004 - QEN71_RS42180 0.39 -0.8 520,060 + QEN71_RS42180 0.43 -0.6 520,060 + QEN71_RS42180 0.43 +0.2 520,061 - QEN71_RS42180 0.43 -0.4 520,061 - QEN71_RS42180 0.43 -0.8 520,061 - QEN71_RS42180 0.43 -0.2 520,062 + QEN71_RS42180 0.43 -1.0 520,062 + QEN71_RS42180 0.43 -0.7 520,062 + QEN71_RS42180 0.43 -1.0 520,063 - QEN71_RS42180 0.43 +0.7 520,063 - QEN71_RS42180 0.43 -0.7 520,063 - QEN71_RS42180 0.43 -1.4 520,092 - QEN71_RS42180 0.45 -0.2 520,130 - QEN71_RS42180 0.48 -0.8 520,130 - QEN71_RS42180 0.48 +0.3 520,130 - QEN71_RS42180 0.48 +0.3 520,197 - QEN71_RS42180 0.52 -0.1 520,197 - QEN71_RS42180 0.52 -0.3 520,469 - QEN71_RS42180 0.70 -0.4 520,540 + QEN71_RS42180 0.74 -1.5 520,541 - QEN71_RS42180 0.74 -0.8 520,559 + QEN71_RS42180 0.76 +1.0 520,559 + QEN71_RS42180 0.76 -1.9 520,559 + QEN71_RS42180 0.76 -1.8 520,559 + QEN71_RS42180 0.76 +1.2 520,559 + QEN71_RS42180 0.76 +1.1 520,559 + QEN71_RS42180 0.76 -0.5 520,560 - QEN71_RS42180 0.76 +0.1 520,616 + QEN71_RS42180 0.79 +0.3 520,616 + QEN71_RS42180 0.79 +0.6 520,616 + QEN71_RS42180 0.79 +1.5 520,617 - QEN71_RS42180 0.79 -0.8 520,617 - QEN71_RS42180 0.79 +1.6 520,617 - QEN71_RS42180 0.79 +0.3 520,617 - QEN71_RS42180 0.79 -0.7 520,756 + QEN71_RS42180 0.88 -1.3 520,756 + QEN71_RS42180 0.88 +0.4 520,757 - QEN71_RS42180 0.88 -0.2 520,931 + -0.5 520,999 + -0.5 520,999 + +0.5 521,133 + QEN71_RS42185 0.15 -1.9 521,133 + QEN71_RS42185 0.15 -0.0 521,133 + QEN71_RS42185 0.15 -0.1 521,134 - QEN71_RS42185 0.16 -0.2 521,134 - QEN71_RS42185 0.16 -0.2 521,139 + QEN71_RS42185 0.16 +0.0 521,178 - QEN71_RS42185 0.19 +1.2 521,178 - QEN71_RS42185 0.19 -1.6 521,188 - QEN71_RS42185 0.20 +0.5 521,318 - QEN71_RS42185 0.29 +0.3 521,336 - QEN71_RS42185 0.31 +0.5 521,336 - QEN71_RS42185 0.31 +0.5 521,336 - QEN71_RS42185 0.31 -1.3 521,410 - QEN71_RS42185 0.36 -0.8 521,410 - QEN71_RS42185 0.36 -1.4 521,414 + QEN71_RS42185 0.36 -0.0 521,415 - QEN71_RS42185 0.36 +0.5 521,450 + QEN71_RS42185 0.39 -0.5 521,451 - QEN71_RS42185 0.39 -2.0 521,451 - QEN71_RS42185 0.39 -2.0 521,451 - QEN71_RS42185 0.39 -1.7 521,451 - QEN71_RS42185 0.39 -0.1 521,586 + QEN71_RS42185 0.49 -0.2 521,586 + QEN71_RS42185 0.49 +0.9 521,587 - QEN71_RS42185 0.49 -0.8 521,587 - QEN71_RS42185 0.49 -0.3 521,626 - QEN71_RS42185 0.52 -0.2 521,786 - QEN71_RS42185 0.64 +1.5 521,837 - QEN71_RS42185 0.68 +0.9 521,966 + QEN71_RS42185 0.77 +1.4 522,042 + QEN71_RS42185 0.83 +1.0 522,172 - -0.8 522,172 - +0.2 522,209 + +0.2 522,209 + +0.0 522,209 + +1.0 522,209 + -1.0 522,209 + -1.5 522,210 - -0.9 522,210 - -0.8 522,293 - +0.0 522,401 + QEN71_RS42190 0.17 +0.0 522,406 - QEN71_RS42190 0.18 -1.2 522,406 - QEN71_RS42190 0.18 -1.7 522,412 + QEN71_RS42190 0.20 +0.3 522,412 + QEN71_RS42190 0.20 -0.8 522,412 + QEN71_RS42190 0.20 -0.7 522,413 - QEN71_RS42190 0.20 -0.0 522,428 + QEN71_RS42190 0.24 -0.7 522,428 + QEN71_RS42190 0.24 -1.0 522,428 + QEN71_RS42190 0.24 +1.2 522,428 + QEN71_RS42190 0.24 +1.0 522,428 + QEN71_RS42190 0.24 -0.2 522,428 + QEN71_RS42190 0.24 +0.2 522,429 - QEN71_RS42190 0.24 -1.0 522,429 - QEN71_RS42190 0.24 +0.0 522,429 - QEN71_RS42190 0.24 +0.2 522,429 - QEN71_RS42190 0.24 +0.3 522,531 + QEN71_RS42190 0.48 -0.8 522,709 - -0.6 522,709 - -0.8 522,713 + -0.1 522,714 - -0.7 522,733 + +0.0 522,733 + -0.1 522,734 - -1.2 522,734 - -0.4 523,061 + +0.0 523,178 + QEN71_RS42200 0.47 -0.9
Or see this region's nucleotide sequence