Experiment: LB_noSalt with Chloride 200 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt QEN71_RS19070 and QEN71_RS19075 are separated by 147 nucleotides QEN71_RS19075 and QEN71_RS19080 are separated by 128 nucleotides QEN71_RS19080 and QEN71_RS19085 are separated by 75 nucleotides
QEN71_RS19070: QEN71_RS19070 - SDR family oxidoreductase, at 4,242,893 to 4,243,144
_RS19070
QEN71_RS19075: QEN71_RS19075 - hypothetical protein, at 4,243,292 to 4,243,888
_RS19075
QEN71_RS19080: QEN71_RS19080 - HU family DNA-binding protein, at 4,244,017 to 4,244,295
_RS19080
QEN71_RS19085: QEN71_RS19085 - alpha/beta hydrolase, at 4,244,371 to 4,245,204
_RS19085
Position (kb)
4244
4245 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 4243.064 kb on + strand, within QEN71_RS19070 at 4243.065 kb on - strand, within QEN71_RS19070 at 4243.094 kb on + strand, within QEN71_RS19070 at 4243.094 kb on + strand, within QEN71_RS19070 at 4243.094 kb on + strand, within QEN71_RS19070 at 4243.095 kb on - strand, within QEN71_RS19070 at 4243.095 kb on - strand, within QEN71_RS19070 at 4243.120 kb on - strand at 4243.143 kb on - strand at 4243.170 kb on + strand at 4243.170 kb on + strand at 4243.170 kb on + strand at 4243.171 kb on - strand at 4243.171 kb on - strand at 4243.175 kb on - strand at 4243.175 kb on - strand at 4243.179 kb on + strand at 4243.180 kb on - strand at 4243.180 kb on - strand at 4243.180 kb on - strand at 4243.181 kb on + strand at 4243.181 kb on + strand at 4243.181 kb on + strand at 4243.181 kb on + strand at 4243.182 kb on - strand at 4243.182 kb on - strand at 4243.185 kb on + strand at 4243.333 kb on + strand at 4243.334 kb on - strand at 4243.351 kb on + strand at 4243.351 kb on + strand at 4243.446 kb on - strand, within QEN71_RS19075 at 4243.446 kb on - strand, within QEN71_RS19075 at 4243.446 kb on - strand, within QEN71_RS19075 at 4243.446 kb on - strand, within QEN71_RS19075 at 4243.446 kb on - strand, within QEN71_RS19075 at 4243.447 kb on + strand, within QEN71_RS19075 at 4243.447 kb on + strand, within QEN71_RS19075 at 4243.447 kb on + strand, within QEN71_RS19075 at 4243.447 kb on + strand, within QEN71_RS19075 at 4243.447 kb on + strand, within QEN71_RS19075 at 4243.447 kb on + strand, within QEN71_RS19075 at 4243.448 kb on - strand, within QEN71_RS19075 at 4243.448 kb on - strand, within QEN71_RS19075 at 4243.448 kb on - strand, within QEN71_RS19075 at 4243.448 kb on - strand, within QEN71_RS19075 at 4243.448 kb on - strand, within QEN71_RS19075 at 4243.524 kb on + strand, within QEN71_RS19075 at 4243.524 kb on + strand, within QEN71_RS19075 at 4243.524 kb on + strand, within QEN71_RS19075 at 4243.525 kb on - strand, within QEN71_RS19075 at 4243.525 kb on - strand, within QEN71_RS19075 at 4243.536 kb on + strand, within QEN71_RS19075 at 4243.536 kb on + strand, within QEN71_RS19075 at 4243.536 kb on + strand, within QEN71_RS19075 at 4243.536 kb on + strand, within QEN71_RS19075 at 4243.537 kb on - strand, within QEN71_RS19075 at 4243.537 kb on - strand, within QEN71_RS19075 at 4243.537 kb on - strand, within QEN71_RS19075 at 4243.537 kb on - strand, within QEN71_RS19075 at 4243.537 kb on - strand, within QEN71_RS19075 at 4243.537 kb on - strand, within QEN71_RS19075 at 4243.537 kb on - strand, within QEN71_RS19075 at 4243.540 kb on + strand, within QEN71_RS19075 at 4243.540 kb on + strand, within QEN71_RS19075 at 4243.541 kb on - strand, within QEN71_RS19075 at 4243.568 kb on + strand, within QEN71_RS19075 at 4243.568 kb on + strand, within QEN71_RS19075 at 4243.569 kb on - strand, within QEN71_RS19075 at 4243.569 kb on - strand, within QEN71_RS19075 at 4243.569 kb on - strand, within QEN71_RS19075 at 4243.569 kb on - strand, within QEN71_RS19075 at 4243.569 kb on - strand, within QEN71_RS19075 at 4243.569 kb on - strand, within QEN71_RS19075 at 4243.831 kb on + strand at 4243.831 kb on + strand at 4243.832 kb on - strand at 4243.833 kb on + strand at 4243.833 kb on + strand at 4243.833 kb on + strand at 4244.088 kb on + strand, within QEN71_RS19080 at 4244.111 kb on + strand, within QEN71_RS19080 at 4244.199 kb on + strand, within QEN71_RS19080 at 4244.208 kb on + strand, within QEN71_RS19080 at 4244.289 kb on + strand at 4244.289 kb on + strand at 4244.463 kb on - strand, within QEN71_RS19085 at 4244.463 kb on - strand, within QEN71_RS19085 at 4244.463 kb on - strand, within QEN71_RS19085 at 4244.682 kb on + strand, within QEN71_RS19085 at 4244.818 kb on + strand, within QEN71_RS19085 at 4244.818 kb on + strand, within QEN71_RS19085 at 4244.818 kb on + strand, within QEN71_RS19085 at 4244.819 kb on - strand, within QEN71_RS19085 at 4244.819 kb on - strand, within QEN71_RS19085 at 4244.819 kb on - strand, within QEN71_RS19085 at 4244.999 kb on + strand, within QEN71_RS19085 at 4245.000 kb on - strand, within QEN71_RS19085 at 4245.030 kb on + strand, within QEN71_RS19085 at 4245.031 kb on - strand, within QEN71_RS19085 at 4245.116 kb on + strand, within QEN71_RS19085 at 4245.117 kb on - strand, within QEN71_RS19085 at 4245.121 kb on + strand at 4245.121 kb on + strand at 4245.121 kb on + strand at 4245.122 kb on - strand at 4245.122 kb on - strand at 4245.122 kb on - strand at 4245.142 kb on + strand at 4245.142 kb on + strand at 4245.143 kb on - strand at 4245.182 kb on - strand at 4245.233 kb on - strand at 4245.271 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_noSalt with Chloride 200 mM remove 4,243,064 + QEN71_RS19070 0.68 -0.1 4,243,065 - QEN71_RS19070 0.68 +0.6 4,243,094 + QEN71_RS19070 0.80 -1.0 4,243,094 + QEN71_RS19070 0.80 -0.4 4,243,094 + QEN71_RS19070 0.80 +0.5 4,243,095 - QEN71_RS19070 0.80 +0.7 4,243,095 - QEN71_RS19070 0.80 -0.4 4,243,120 - -0.5 4,243,143 - -1.3 4,243,170 + -1.6 4,243,170 + -0.5 4,243,170 + +1.2 4,243,171 - -1.0 4,243,171 - -0.1 4,243,175 - -0.5 4,243,175 - -0.8 4,243,179 + +0.3 4,243,180 - -1.2 4,243,180 - -1.9 4,243,180 - -0.2 4,243,181 + +0.0 4,243,181 + -0.6 4,243,181 + +0.5 4,243,181 + -1.6 4,243,182 - -0.4 4,243,182 - +0.5 4,243,185 + +1.9 4,243,333 + -0.2 4,243,334 - -1.7 4,243,351 + -0.1 4,243,351 + -0.8 4,243,446 - QEN71_RS19075 0.26 -0.1 4,243,446 - QEN71_RS19075 0.26 +0.5 4,243,446 - QEN71_RS19075 0.26 -1.3 4,243,446 - QEN71_RS19075 0.26 -0.2 4,243,446 - QEN71_RS19075 0.26 -1.2 4,243,447 + QEN71_RS19075 0.26 +0.5 4,243,447 + QEN71_RS19075 0.26 -1.2 4,243,447 + QEN71_RS19075 0.26 -1.0 4,243,447 + QEN71_RS19075 0.26 -0.1 4,243,447 + QEN71_RS19075 0.26 +0.2 4,243,447 + QEN71_RS19075 0.26 +0.4 4,243,448 - QEN71_RS19075 0.26 +2.2 4,243,448 - QEN71_RS19075 0.26 -0.7 4,243,448 - QEN71_RS19075 0.26 -0.1 4,243,448 - QEN71_RS19075 0.26 +0.9 4,243,448 - QEN71_RS19075 0.26 +1.6 4,243,524 + QEN71_RS19075 0.39 -1.1 4,243,524 + QEN71_RS19075 0.39 +0.5 4,243,524 + QEN71_RS19075 0.39 -0.4 4,243,525 - QEN71_RS19075 0.39 -0.3 4,243,525 - QEN71_RS19075 0.39 +0.9 4,243,536 + QEN71_RS19075 0.41 +0.5 4,243,536 + QEN71_RS19075 0.41 -0.5 4,243,536 + QEN71_RS19075 0.41 -1.2 4,243,536 + QEN71_RS19075 0.41 -0.5 4,243,537 - QEN71_RS19075 0.41 -0.3 4,243,537 - QEN71_RS19075 0.41 -0.0 4,243,537 - QEN71_RS19075 0.41 -0.1 4,243,537 - QEN71_RS19075 0.41 +0.2 4,243,537 - QEN71_RS19075 0.41 +1.1 4,243,537 - QEN71_RS19075 0.41 +0.4 4,243,537 - QEN71_RS19075 0.41 -0.2 4,243,540 + QEN71_RS19075 0.42 +0.1 4,243,540 + QEN71_RS19075 0.42 +0.6 4,243,541 - QEN71_RS19075 0.42 +0.5 4,243,568 + QEN71_RS19075 0.46 +0.3 4,243,568 + QEN71_RS19075 0.46 -2.8 4,243,569 - QEN71_RS19075 0.46 -0.5 4,243,569 - QEN71_RS19075 0.46 -0.5 4,243,569 - QEN71_RS19075 0.46 +0.4 4,243,569 - QEN71_RS19075 0.46 +1.0 4,243,569 - QEN71_RS19075 0.46 +0.6 4,243,569 - QEN71_RS19075 0.46 -1.2 4,243,831 + -0.7 4,243,831 + +0.5 4,243,832 - +0.6 4,243,833 + +0.2 4,243,833 + +0.2 4,243,833 + -0.3 4,244,088 + QEN71_RS19080 0.25 -0.9 4,244,111 + QEN71_RS19080 0.34 -1.4 4,244,199 + QEN71_RS19080 0.65 +0.6 4,244,208 + QEN71_RS19080 0.68 +0.1 4,244,289 + +0.7 4,244,289 + -2.2 4,244,463 - QEN71_RS19085 0.11 +0.1 4,244,463 - QEN71_RS19085 0.11 -1.0 4,244,463 - QEN71_RS19085 0.11 -0.2 4,244,682 + QEN71_RS19085 0.37 +1.2 4,244,818 + QEN71_RS19085 0.54 +1.5 4,244,818 + QEN71_RS19085 0.54 -0.2 4,244,818 + QEN71_RS19085 0.54 +0.1 4,244,819 - QEN71_RS19085 0.54 +0.1 4,244,819 - QEN71_RS19085 0.54 -0.1 4,244,819 - QEN71_RS19085 0.54 +0.2 4,244,999 + QEN71_RS19085 0.75 -0.1 4,245,000 - QEN71_RS19085 0.75 +0.1 4,245,030 + QEN71_RS19085 0.79 +1.6 4,245,031 - QEN71_RS19085 0.79 -0.7 4,245,116 + QEN71_RS19085 0.89 -0.3 4,245,117 - QEN71_RS19085 0.89 +0.9 4,245,121 + +1.3 4,245,121 + +1.5 4,245,121 + +0.4 4,245,122 - -0.1 4,245,122 - -0.2 4,245,122 - +2.2 4,245,142 + -0.1 4,245,142 + +0.9 4,245,143 - +0.7 4,245,182 - +0.1 4,245,233 - +0.0 4,245,271 + +0.7
Or see this region's nucleotide sequence