Experiment: LB_noSalt with Chloride 200 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt QEN71_RS02150 and QEN71_RS02155 are separated by 37 nucleotides QEN71_RS02155 and QEN71_RS02160 are separated by 10 nucleotides QEN71_RS02160 and QEN71_RS02165 are separated by 15 nucleotides
QEN71_RS02150: QEN71_RS02150 - lysophospholipid acyltransferase family protein, at 482,392 to 483,150
_RS02150
QEN71_RS02155: QEN71_RS02155 - D-glycero-beta-D-manno-heptose 1,7-bisphosphate 7-phosphatase, at 483,188 to 483,745
_RS02155
QEN71_RS02160: QEN71_RS02160 - glycine--tRNA ligase subunit beta, at 483,756 to 485,855
_RS02160
QEN71_RS02165: QEN71_RS02165 - glycine--tRNA ligase subunit alpha, at 485,871 to 486,878
_RS02165
Position (kb) 483
484
485
486 Strain fitness (log2 ratio) -1
0
1
2 at 482.837 kb on + strand, within QEN71_RS02150 at 482.838 kb on - strand, within QEN71_RS02150 at 482.838 kb on - strand, within QEN71_RS02150 at 482.838 kb on - strand, within QEN71_RS02150 at 482.861 kb on + strand, within QEN71_RS02150 at 482.861 kb on + strand, within QEN71_RS02150 at 482.862 kb on - strand, within QEN71_RS02150 at 482.862 kb on - strand, within QEN71_RS02150 at 482.862 kb on - strand, within QEN71_RS02150 at 483.179 kb on + strand at 483.179 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_noSalt with Chloride 200 mM remove 482,837 + QEN71_RS02150 0.59 -0.1 482,838 - QEN71_RS02150 0.59 -1.3 482,838 - QEN71_RS02150 0.59 -0.9 482,838 - QEN71_RS02150 0.59 -0.1 482,861 + QEN71_RS02150 0.62 -1.4 482,861 + QEN71_RS02150 0.62 -0.1 482,862 - QEN71_RS02150 0.62 +0.9 482,862 - QEN71_RS02150 0.62 -1.7 482,862 - QEN71_RS02150 0.62 -1.4 483,179 + -1.7 483,179 + +2.2
Or see this region's nucleotide sequence