Experiment: LB_noSalt with Zinc 0.5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt QEN71_RS30235 and QEN71_RS30240 overlap by 32 nucleotides QEN71_RS30240 and QEN71_RS30245 are separated by 154 nucleotides QEN71_RS30245 and QEN71_RS30250 are separated by 153 nucleotides
QEN71_RS30235: QEN71_RS30235 - alpha/beta fold hydrolase, at 132,957 to 133,925
_RS30235
QEN71_RS30240: QEN71_RS30240 - hypothetical protein, at 133,894 to 134,169
_RS30240
QEN71_RS30245: QEN71_RS30245 - hypothetical protein, at 134,324 to 134,605
_RS30245
QEN71_RS30250: QEN71_RS30250 - glycine zipper 2TM domain-containing protein, at 134,759 to 135,532
_RS30250
Position (kb) 133
134
135 Strain fitness (log2 ratio) -2
-1
0
1
2 at 133.017 kb on + strand at 133.018 kb on - strand at 133.018 kb on - strand at 133.018 kb on - strand at 133.019 kb on + strand at 133.020 kb on - strand at 133.297 kb on - strand, within QEN71_RS30235 at 133.472 kb on + strand, within QEN71_RS30235 at 133.518 kb on + strand, within QEN71_RS30235 at 133.519 kb on - strand, within QEN71_RS30235 at 133.611 kb on + strand, within QEN71_RS30235 at 133.611 kb on + strand, within QEN71_RS30235 at 133.612 kb on - strand, within QEN71_RS30235 at 133.630 kb on - strand, within QEN71_RS30235 at 133.630 kb on - strand, within QEN71_RS30235 at 133.895 kb on + strand at 134.092 kb on + strand, within QEN71_RS30240 at 134.093 kb on - strand, within QEN71_RS30240 at 134.138 kb on - strand, within QEN71_RS30240 at 134.207 kb on + strand at 134.208 kb on - strand at 134.290 kb on + strand at 134.314 kb on - strand at 134.415 kb on + strand, within QEN71_RS30245 at 134.421 kb on + strand, within QEN71_RS30245 at 134.421 kb on + strand, within QEN71_RS30245 at 134.421 kb on + strand, within QEN71_RS30245 at 134.422 kb on - strand, within QEN71_RS30245 at 134.422 kb on - strand, within QEN71_RS30245 at 134.467 kb on - strand, within QEN71_RS30245 at 134.493 kb on + strand, within QEN71_RS30245 at 134.494 kb on - strand, within QEN71_RS30245 at 134.608 kb on + strand at 134.668 kb on + strand at 134.668 kb on + strand at 134.668 kb on + strand at 134.669 kb on - strand at 134.720 kb on + strand at 134.724 kb on - strand at 134.724 kb on - strand at 134.724 kb on - strand at 134.776 kb on + strand at 134.777 kb on - strand at 134.777 kb on - strand at 134.787 kb on - strand at 134.829 kb on + strand at 134.829 kb on + strand at 134.829 kb on + strand at 134.830 kb on - strand at 134.830 kb on - strand at 134.830 kb on - strand at 134.844 kb on + strand, within QEN71_RS30250 at 134.878 kb on - strand, within QEN71_RS30250 at 134.878 kb on - strand, within QEN71_RS30250 at 134.878 kb on - strand, within QEN71_RS30250
Per-strain Table
Position Strand Gene LocusTag Fraction LB_noSalt with Zinc 0.5 mM remove 133,017 + -0.4 133,018 - +0.8 133,018 - -2.4 133,018 - +0.7 133,019 + -0.5 133,020 - +0.2 133,297 - QEN71_RS30235 0.35 -0.4 133,472 + QEN71_RS30235 0.53 -0.0 133,518 + QEN71_RS30235 0.58 -0.2 133,519 - QEN71_RS30235 0.58 +0.1 133,611 + QEN71_RS30235 0.67 -0.6 133,611 + QEN71_RS30235 0.67 +0.0 133,612 - QEN71_RS30235 0.68 -0.1 133,630 - QEN71_RS30235 0.69 +1.8 133,630 - QEN71_RS30235 0.69 +0.3 133,895 + -1.9 134,092 + QEN71_RS30240 0.72 -0.2 134,093 - QEN71_RS30240 0.72 -0.4 134,138 - QEN71_RS30240 0.88 -1.6 134,207 + -0.4 134,208 - -1.1 134,290 + -0.3 134,314 - +1.2 134,415 + QEN71_RS30245 0.32 -0.0 134,421 + QEN71_RS30245 0.34 +0.0 134,421 + QEN71_RS30245 0.34 -0.2 134,421 + QEN71_RS30245 0.34 -1.0 134,422 - QEN71_RS30245 0.35 -1.2 134,422 - QEN71_RS30245 0.35 -0.7 134,467 - QEN71_RS30245 0.51 +1.4 134,493 + QEN71_RS30245 0.60 +0.2 134,494 - QEN71_RS30245 0.60 -1.8 134,608 + -0.2 134,668 + -0.4 134,668 + -0.2 134,668 + -0.6 134,669 - -0.1 134,720 + +0.2 134,724 - +0.6 134,724 - -0.8 134,724 - -0.2 134,776 + -0.2 134,777 - +0.3 134,777 - -0.8 134,787 - -0.4 134,829 + +0.9 134,829 + +0.2 134,829 + +0.2 134,830 - -2.5 134,830 - -1.2 134,830 - +0.2 134,844 + QEN71_RS30250 0.11 -2.0 134,878 - QEN71_RS30250 0.15 -1.8 134,878 - QEN71_RS30250 0.15 -0.2 134,878 - QEN71_RS30250 0.15 +0.3
Or see this region's nucleotide sequence