Experiment: LB_noSalt with Zinc 0.5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt QEN71_RS29995 and QEN71_RS30000 overlap by 4 nucleotides QEN71_RS30000 and QEN71_RS30005 overlap by 4 nucleotides QEN71_RS30005 and QEN71_RS30010 are separated by 48 nucleotides
QEN71_RS29995: QEN71_RS29995 - branched-chain amino acid ABC transporter ATP-binding protein/permease, at 75,388 to 77,940
_RS29995
QEN71_RS30000: QEN71_RS30000 - shikimate dehydrogenase, at 77,937 to 78,779
_RS30000
QEN71_RS30005: QEN71_RS30005 - SDR family NAD(P)-dependent oxidoreductase, at 78,776 to 79,516
_RS30005
QEN71_RS30010: QEN71_RS30010 - FAD-binding protein, at 79,565 to 81,334
_RS30010
Position (kb) 78
79
80 Strain fitness (log2 ratio) -2
-1
0
1
2 at 77.806 kb on + strand at 77.807 kb on - strand at 77.807 kb on - strand at 77.917 kb on - strand at 78.056 kb on - strand, within QEN71_RS30000 at 78.257 kb on + strand, within QEN71_RS30000 at 78.258 kb on - strand, within QEN71_RS30000 at 78.258 kb on - strand, within QEN71_RS30000 at 78.455 kb on + strand, within QEN71_RS30000 at 78.455 kb on + strand, within QEN71_RS30000 at 78.456 kb on - strand, within QEN71_RS30000 at 78.456 kb on - strand, within QEN71_RS30000 at 78.945 kb on + strand, within QEN71_RS30005 at 79.511 kb on + strand at 79.511 kb on + strand at 79.512 kb on - strand at 79.534 kb on - strand at 79.714 kb on + strand at 79.714 kb on + strand at 79.715 kb on - strand at 79.715 kb on - strand at 79.715 kb on - strand at 79.715 kb on - strand at 79.715 kb on - strand at 79.940 kb on + strand, within QEN71_RS30010 at 79.940 kb on + strand, within QEN71_RS30010 at 80.159 kb on + strand, within QEN71_RS30010 at 80.234 kb on + strand, within QEN71_RS30010 at 80.354 kb on - strand, within QEN71_RS30010
Per-strain Table
Position Strand Gene LocusTag Fraction LB_noSalt with Zinc 0.5 mM remove 77,806 + -0.3 77,807 - -0.0 77,807 - -1.0 77,917 - -2.0 78,056 - QEN71_RS30000 0.14 -0.6 78,257 + QEN71_RS30000 0.38 -0.2 78,258 - QEN71_RS30000 0.38 +0.4 78,258 - QEN71_RS30000 0.38 -0.2 78,455 + QEN71_RS30000 0.61 +1.2 78,455 + QEN71_RS30000 0.61 +0.3 78,456 - QEN71_RS30000 0.62 -1.2 78,456 - QEN71_RS30000 0.62 +0.2 78,945 + QEN71_RS30005 0.23 -1.6 79,511 + -1.2 79,511 + +0.2 79,512 - -0.2 79,534 - -0.2 79,714 + +1.2 79,714 + -0.8 79,715 - +0.3 79,715 - -0.9 79,715 - +1.9 79,715 - -0.7 79,715 - -1.3 79,940 + QEN71_RS30010 0.21 -1.7 79,940 + QEN71_RS30010 0.21 -1.2 80,159 + QEN71_RS30010 0.34 -0.6 80,234 + QEN71_RS30010 0.38 +0.2 80,354 - QEN71_RS30010 0.45 +0.8
Or see this region's nucleotide sequence