Experiment: LB_noSalt with Cobalt chloride 0.25 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt QEN71_RS32745 and QEN71_RS32750 are separated by 426 nucleotides QEN71_RS32750 and QEN71_RS32755 overlap by 26 nucleotides QEN71_RS32755 and QEN71_RS32760 are separated by 395 nucleotides
QEN71_RS32745: QEN71_RS32745 - DUF447 family protein, at 680,123 to 680,734
_RS32745
QEN71_RS32750: QEN71_RS32750 - hypothetical protein, at 681,161 to 681,463
_RS32750
QEN71_RS32755: QEN71_RS32755 - pyrroloquinoline quinone precursor peptide PqqA, at 681,438 to 681,512
_RS32755
QEN71_RS32760: QEN71_RS32760 - form I ribulose bisphosphate carboxylase large subunit, at 681,908 to 683,398
_RS32760
Position (kb) 681
682 Strain fitness (log2 ratio) -1
0
1 at 680.462 kb on - strand, within QEN71_RS32745 at 680.480 kb on + strand, within QEN71_RS32745 at 680.491 kb on + strand, within QEN71_RS32745 at 680.556 kb on - strand, within QEN71_RS32745 at 680.751 kb on + strand at 680.771 kb on + strand at 680.833 kb on - strand at 680.833 kb on - strand at 681.089 kb on + strand at 681.090 kb on - strand at 681.090 kb on - strand at 681.191 kb on + strand at 681.454 kb on + strand at 681.454 kb on + strand at 681.454 kb on + strand at 681.454 kb on + strand at 681.455 kb on - strand at 681.455 kb on - strand at 681.455 kb on - strand at 681.455 kb on - strand at 681.455 kb on - strand at 681.490 kb on + strand, within QEN71_RS32755 at 681.500 kb on + strand, within QEN71_RS32755 at 681.500 kb on + strand, within QEN71_RS32755 at 681.501 kb on - strand, within QEN71_RS32755 at 681.512 kb on + strand at 681.647 kb on + strand at 681.647 kb on + strand at 681.674 kb on - strand at 681.813 kb on + strand at 681.992 kb on + strand at 682.023 kb on - strand at 682.023 kb on - strand at 682.023 kb on - strand at 682.175 kb on + strand, within QEN71_RS32760 at 682.175 kb on + strand, within QEN71_RS32760 at 682.235 kb on + strand, within QEN71_RS32760 at 682.512 kb on + strand, within QEN71_RS32760 at 682.512 kb on + strand, within QEN71_RS32760
Per-strain Table
Position Strand Gene LocusTag Fraction LB_noSalt with Cobalt chloride 0.25 mM remove 680,462 - QEN71_RS32745 0.55 +0.3 680,480 + QEN71_RS32745 0.58 +1.0 680,491 + QEN71_RS32745 0.60 -1.4 680,556 - QEN71_RS32745 0.71 +0.2 680,751 + -1.2 680,771 + +0.3 680,833 - -0.7 680,833 - +0.0 681,089 + +0.0 681,090 - -0.4 681,090 - +1.2 681,191 + -0.1 681,454 + -0.4 681,454 + -1.3 681,454 + -0.2 681,454 + -0.4 681,455 - +0.4 681,455 - +0.6 681,455 - +0.6 681,455 - -0.8 681,455 - -0.0 681,490 + QEN71_RS32755 0.69 -0.3 681,500 + QEN71_RS32755 0.83 -0.1 681,500 + QEN71_RS32755 0.83 -0.2 681,501 - QEN71_RS32755 0.84 -1.3 681,512 + -0.8 681,647 + +0.5 681,647 + -0.8 681,674 - -1.2 681,813 + -0.8 681,992 + +1.3 682,023 - +0.3 682,023 - +0.3 682,023 - -0.4 682,175 + QEN71_RS32760 0.18 -0.5 682,175 + QEN71_RS32760 0.18 +0.1 682,235 + QEN71_RS32760 0.22 +0.1 682,512 + QEN71_RS32760 0.41 -0.8 682,512 + QEN71_RS32760 0.41 +0.7
Or see this region's nucleotide sequence