Strain Fitness in Paraburkholderia sabiae LMG 24235 around QEN71_RS14050

Experiment: LB_noSalt with Cobalt chloride 0.25 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntQEN71_RS14040 and QEN71_RS14045 are separated by 50 nucleotidesQEN71_RS14045 and QEN71_RS14050 are separated by 142 nucleotidesQEN71_RS14050 and QEN71_RS14055 are separated by 93 nucleotidesQEN71_RS14055 and QEN71_RS14060 are separated by 164 nucleotides QEN71_RS14040: QEN71_RS14040 - phytanoyl-CoA dioxygenase family protein, at 3,133,914 to 3,134,750 _RS14040 QEN71_RS14045: QEN71_RS14045 - ABC transporter substrate-binding protein, at 3,134,801 to 3,135,574 _RS14045 QEN71_RS14050: QEN71_RS14050 - AMP-binding protein, at 3,135,717 to 3,137,342 _RS14050 QEN71_RS14055: QEN71_RS14055 - SDR family NAD(P)-dependent oxidoreductase, at 3,137,436 to 3,138,146 _RS14055 QEN71_RS14060: QEN71_RS14060 - sigma-70 family RNA polymerase sigma factor, at 3,138,311 to 3,138,895 _RS14060 Position (kb) 3135 3136 3137 3138Strain fitness (log2 ratio) -2 -1 0 1at 3134.850 kb on + strandat 3134.850 kb on + strandat 3135.078 kb on + strand, within QEN71_RS14045at 3135.099 kb on + strand, within QEN71_RS14045at 3135.132 kb on + strand, within QEN71_RS14045at 3135.132 kb on + strand, within QEN71_RS14045at 3135.215 kb on - strand, within QEN71_RS14045at 3135.215 kb on - strand, within QEN71_RS14045at 3135.477 kb on + strand, within QEN71_RS14045at 3135.477 kb on + strand, within QEN71_RS14045at 3135.477 kb on + strand, within QEN71_RS14045at 3135.477 kb on + strand, within QEN71_RS14045at 3135.477 kb on + strand, within QEN71_RS14045at 3135.477 kb on + strand, within QEN71_RS14045at 3135.477 kb on + strand, within QEN71_RS14045at 3135.477 kb on + strand, within QEN71_RS14045at 3135.478 kb on - strand, within QEN71_RS14045at 3135.478 kb on - strand, within QEN71_RS14045at 3135.660 kb on + strandat 3135.660 kb on + strandat 3135.660 kb on + strandat 3135.660 kb on + strandat 3135.661 kb on - strandat 3135.661 kb on - strandat 3135.684 kb on - strandat 3135.684 kb on - strandat 3135.804 kb on - strandat 3136.061 kb on - strand, within QEN71_RS14050at 3136.061 kb on - strand, within QEN71_RS14050at 3136.399 kb on + strand, within QEN71_RS14050at 3136.399 kb on + strand, within QEN71_RS14050at 3136.400 kb on - strand, within QEN71_RS14050at 3136.400 kb on - strand, within QEN71_RS14050at 3136.447 kb on + strand, within QEN71_RS14050at 3136.448 kb on - strand, within QEN71_RS14050at 3136.448 kb on - strand, within QEN71_RS14050at 3136.448 kb on - strand, within QEN71_RS14050at 3136.451 kb on + strand, within QEN71_RS14050at 3136.451 kb on + strand, within QEN71_RS14050at 3136.451 kb on + strand, within QEN71_RS14050at 3136.451 kb on + strand, within QEN71_RS14050at 3136.452 kb on - strand, within QEN71_RS14050at 3136.452 kb on - strand, within QEN71_RS14050at 3136.452 kb on - strand, within QEN71_RS14050at 3136.452 kb on - strand, within QEN71_RS14050at 3136.617 kb on - strand, within QEN71_RS14050at 3136.660 kb on + strand, within QEN71_RS14050at 3136.660 kb on + strand, within QEN71_RS14050at 3136.660 kb on + strand, within QEN71_RS14050at 3136.660 kb on + strand, within QEN71_RS14050at 3136.660 kb on + strand, within QEN71_RS14050at 3136.661 kb on - strand, within QEN71_RS14050at 3136.661 kb on - strand, within QEN71_RS14050at 3136.661 kb on - strand, within QEN71_RS14050at 3136.661 kb on - strand, within QEN71_RS14050at 3136.701 kb on - strand, within QEN71_RS14050at 3136.701 kb on - strand, within QEN71_RS14050at 3136.701 kb on - strand, within QEN71_RS14050at 3136.727 kb on - strand, within QEN71_RS14050at 3136.793 kb on - strand, within QEN71_RS14050at 3136.923 kb on + strand, within QEN71_RS14050at 3136.924 kb on - strand, within QEN71_RS14050at 3136.924 kb on - strand, within QEN71_RS14050at 3136.951 kb on + strand, within QEN71_RS14050at 3137.039 kb on - strand, within QEN71_RS14050at 3137.039 kb on - strand, within QEN71_RS14050at 3137.206 kb on + strandat 3137.206 kb on + strandat 3137.207 kb on - strandat 3137.252 kb on - strandat 3137.284 kb on - strandat 3137.284 kb on - strandat 3137.308 kb on + strandat 3137.309 kb on - strandat 3137.309 kb on - strandat 3137.309 kb on - strandat 3137.426 kb on + strandat 3137.427 kb on - strandat 3137.470 kb on + strandat 3137.471 kb on - strandat 3137.675 kb on - strand, within QEN71_RS14055at 3137.675 kb on - strand, within QEN71_RS14055at 3137.903 kb on + strand, within QEN71_RS14055at 3137.904 kb on - strand, within QEN71_RS14055at 3137.954 kb on + strand, within QEN71_RS14055at 3137.986 kb on + strand, within QEN71_RS14055at 3138.179 kb on + strandat 3138.180 kb on - strandat 3138.229 kb on + strandat 3138.229 kb on + strandat 3138.229 kb on + strandat 3138.229 kb on + strandat 3138.229 kb on + strandat 3138.230 kb on - strandat 3138.230 kb on - strandat 3138.230 kb on - strandat 3138.230 kb on - strandat 3138.281 kb on + strandat 3138.282 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction LB_noSalt with Cobalt chloride 0.25 mM
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3,134,850 + -1.3
3,134,850 + +0.6
3,135,078 + QEN71_RS14045 0.36 +1.2
3,135,099 + QEN71_RS14045 0.39 -0.3
3,135,132 + QEN71_RS14045 0.43 +0.3
3,135,132 + QEN71_RS14045 0.43 -1.0
3,135,215 - QEN71_RS14045 0.53 +0.2
3,135,215 - QEN71_RS14045 0.53 -0.3
3,135,477 + QEN71_RS14045 0.87 +0.5
3,135,477 + QEN71_RS14045 0.87 +1.5
3,135,477 + QEN71_RS14045 0.87 -0.1
3,135,477 + QEN71_RS14045 0.87 -0.3
3,135,477 + QEN71_RS14045 0.87 -0.5
3,135,477 + QEN71_RS14045 0.87 -0.2
3,135,477 + QEN71_RS14045 0.87 -2.1
3,135,477 + QEN71_RS14045 0.87 -1.3
3,135,478 - QEN71_RS14045 0.87 -1.0
3,135,478 - QEN71_RS14045 0.87 +0.3
3,135,660 + +0.2
3,135,660 + +1.4
3,135,660 + +0.1
3,135,660 + -0.3
3,135,661 - -1.6
3,135,661 - +1.2
3,135,684 - +0.4
3,135,684 - +0.4
3,135,804 - -0.6
3,136,061 - QEN71_RS14050 0.21 -0.1
3,136,061 - QEN71_RS14050 0.21 -0.5
3,136,399 + QEN71_RS14050 0.42 -2.7
3,136,399 + QEN71_RS14050 0.42 +0.2
3,136,400 - QEN71_RS14050 0.42 -1.2
3,136,400 - QEN71_RS14050 0.42 +0.3
3,136,447 + QEN71_RS14050 0.45 -1.7
3,136,448 - QEN71_RS14050 0.45 +0.5
3,136,448 - QEN71_RS14050 0.45 -1.9
3,136,448 - QEN71_RS14050 0.45 -0.2
3,136,451 + QEN71_RS14050 0.45 -0.9
3,136,451 + QEN71_RS14050 0.45 +1.1
3,136,451 + QEN71_RS14050 0.45 +0.1
3,136,451 + QEN71_RS14050 0.45 -0.2
3,136,452 - QEN71_RS14050 0.45 +0.5
3,136,452 - QEN71_RS14050 0.45 -0.2
3,136,452 - QEN71_RS14050 0.45 -0.6
3,136,452 - QEN71_RS14050 0.45 -0.5
3,136,617 - QEN71_RS14050 0.55 +0.0
3,136,660 + QEN71_RS14050 0.58 +0.8
3,136,660 + QEN71_RS14050 0.58 +0.2
3,136,660 + QEN71_RS14050 0.58 +0.4
3,136,660 + QEN71_RS14050 0.58 -1.1
3,136,660 + QEN71_RS14050 0.58 -1.9
3,136,661 - QEN71_RS14050 0.58 +0.0
3,136,661 - QEN71_RS14050 0.58 +0.3
3,136,661 - QEN71_RS14050 0.58 -0.7
3,136,661 - QEN71_RS14050 0.58 +0.5
3,136,701 - QEN71_RS14050 0.61 -0.8
3,136,701 - QEN71_RS14050 0.61 +0.0
3,136,701 - QEN71_RS14050 0.61 +0.5
3,136,727 - QEN71_RS14050 0.62 +0.5
3,136,793 - QEN71_RS14050 0.66 -0.0
3,136,923 + QEN71_RS14050 0.74 -0.1
3,136,924 - QEN71_RS14050 0.74 +0.4
3,136,924 - QEN71_RS14050 0.74 +0.3
3,136,951 + QEN71_RS14050 0.76 -0.0
3,137,039 - QEN71_RS14050 0.81 -0.2
3,137,039 - QEN71_RS14050 0.81 -1.3
3,137,206 + +0.8
3,137,206 + +0.2
3,137,207 - +0.8
3,137,252 - -0.0
3,137,284 - -0.6
3,137,284 - -0.1
3,137,308 + -0.8
3,137,309 - +0.8
3,137,309 - -0.6
3,137,309 - -1.4
3,137,426 + -0.2
3,137,427 - +0.1
3,137,470 + -0.6
3,137,471 - +0.1
3,137,675 - QEN71_RS14055 0.34 +0.8
3,137,675 - QEN71_RS14055 0.34 +1.6
3,137,903 + QEN71_RS14055 0.66 -0.3
3,137,904 - QEN71_RS14055 0.66 -0.9
3,137,954 + QEN71_RS14055 0.73 -1.0
3,137,986 + QEN71_RS14055 0.77 -1.2
3,138,179 + -0.2
3,138,180 - +0.0
3,138,229 + +0.8
3,138,229 + +0.8
3,138,229 + +0.6
3,138,229 + -0.2
3,138,229 + +0.2
3,138,230 - +0.4
3,138,230 - +0.2
3,138,230 - -1.1
3,138,230 - -0.7
3,138,281 + +0.4
3,138,282 - +0.1

Or see this region's nucleotide sequence