Experiment: LB_noSalt with Cobalt chloride 0.25 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt QEN71_RS12745 and QEN71_RS12750 are separated by 74 nucleotides QEN71_RS12750 and QEN71_RS12755 are separated by 64 nucleotides QEN71_RS12755 and QEN71_RS12760 are separated by 422 nucleotides
QEN71_RS12745: QEN71_RS12745 - ectoine/hydroxyectoine ABC transporter permease subunit EhuC, at 2,851,027 to 2,851,686
_RS12745
QEN71_RS12750: QEN71_RS12750 - ectoine/hydroxyectoine ABC transporter substrate-binding protein EhuB, at 2,851,761 to 2,852,600
_RS12750
QEN71_RS12755: QEN71_RS12755 - ectoine/hydroxyectoine ABC transporter ATP-binding protein EhuA, at 2,852,665 to 2,853,567
_RS12755
QEN71_RS12760: QEN71_RS12760 - ectoine hydrolase DoeA, at 2,853,990 to 2,855,210
_RS12760
Position (kb)
2852
2853
2854 Strain fitness (log2 ratio)
-1
0
1 at 2851.730 kb on - strand at 2851.740 kb on - strand at 2852.192 kb on - strand, within QEN71_RS12750 at 2852.263 kb on + strand, within QEN71_RS12750 at 2852.263 kb on + strand, within QEN71_RS12750 at 2852.737 kb on + strand at 2852.738 kb on - strand at 2853.634 kb on - strand at 2853.783 kb on + strand at 2853.784 kb on - strand at 2853.787 kb on + strand at 2853.787 kb on + strand at 2853.834 kb on - strand at 2853.859 kb on - strand at 2853.890 kb on - strand at 2853.930 kb on + strand at 2853.959 kb on - strand at 2854.161 kb on + strand, within QEN71_RS12760 at 2854.180 kb on - strand, within QEN71_RS12760 at 2854.287 kb on - strand, within QEN71_RS12760 at 2854.308 kb on + strand, within QEN71_RS12760 at 2854.308 kb on + strand, within QEN71_RS12760 at 2854.410 kb on + strand, within QEN71_RS12760 at 2854.410 kb on + strand, within QEN71_RS12760 at 2854.530 kb on + strand, within QEN71_RS12760
Per-strain Table
Position Strand Gene LocusTag Fraction LB_noSalt with Cobalt chloride 0.25 mM remove 2,851,730 - +0.8 2,851,740 - +0.5 2,852,192 - QEN71_RS12750 0.51 +0.6 2,852,263 + QEN71_RS12750 0.60 -0.3 2,852,263 + QEN71_RS12750 0.60 -1.0 2,852,737 + +1.4 2,852,738 - -0.7 2,853,634 - +0.3 2,853,783 + -0.7 2,853,784 - -0.6 2,853,787 + +0.8 2,853,787 + +0.3 2,853,834 - +1.3 2,853,859 - +0.5 2,853,890 - -0.9 2,853,930 + -0.2 2,853,959 - +0.5 2,854,161 + QEN71_RS12760 0.14 +0.9 2,854,180 - QEN71_RS12760 0.16 -0.2 2,854,287 - QEN71_RS12760 0.24 +0.5 2,854,308 + QEN71_RS12760 0.26 +0.6 2,854,308 + QEN71_RS12760 0.26 -0.2 2,854,410 + QEN71_RS12760 0.34 +1.4 2,854,410 + QEN71_RS12760 0.34 +1.4 2,854,530 + QEN71_RS12760 0.44 +0.8
Or see this region's nucleotide sequence