Experiment: LB_noSalt with Cobalt chloride 0.25 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt QEN71_RS08485 and QEN71_RS08490 are separated by 47 nucleotides QEN71_RS08490 and QEN71_RS08495 are separated by 107 nucleotides QEN71_RS08495 and QEN71_RS08500 overlap by 31 nucleotides
QEN71_RS08485: QEN71_RS08485 - glycosyltransferase family 4 protein, at 1,894,830 to 1,895,918
_RS08485
QEN71_RS08490: QEN71_RS08490 - IS30 family transposase, at 1,895,966 to 1,896,650
_RS08490
QEN71_RS08495: QEN71_RS08495 - acyltransferase family protein, at 1,896,758 to 1,897,738
_RS08495
QEN71_RS08500: QEN71_RS08500 - IS5 family transposase, at 1,897,708 to 1,898,519
_RS08500
Position (kb)
1896
1897
1898 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 1895.797 kb on - strand, within QEN71_RS08485 at 1895.821 kb on + strand at 1895.856 kb on + strand at 1895.857 kb on - strand at 1895.936 kb on - strand at 1896.315 kb on + strand, within QEN71_RS08490 at 1896.315 kb on + strand, within QEN71_RS08490 at 1896.316 kb on - strand, within QEN71_RS08490 at 1896.372 kb on - strand, within QEN71_RS08490 at 1896.372 kb on - strand, within QEN71_RS08490 at 1896.376 kb on - strand, within QEN71_RS08490 at 1896.383 kb on + strand, within QEN71_RS08490 at 1896.383 kb on + strand, within QEN71_RS08490 at 1896.426 kb on - strand, within QEN71_RS08490 at 1896.429 kb on + strand, within QEN71_RS08490 at 1896.429 kb on + strand, within QEN71_RS08490 at 1896.429 kb on + strand, within QEN71_RS08490 at 1896.429 kb on + strand, within QEN71_RS08490 at 1896.429 kb on + strand, within QEN71_RS08490 at 1896.429 kb on + strand, within QEN71_RS08490 at 1896.430 kb on - strand, within QEN71_RS08490 at 1896.787 kb on + strand at 1896.788 kb on - strand at 1896.821 kb on + strand at 1896.984 kb on + strand, within QEN71_RS08495 at 1896.985 kb on - strand, within QEN71_RS08495 at 1896.985 kb on - strand, within QEN71_RS08495 at 1896.985 kb on - strand, within QEN71_RS08495 at 1896.985 kb on - strand, within QEN71_RS08495 at 1897.067 kb on - strand, within QEN71_RS08495 at 1897.144 kb on + strand, within QEN71_RS08495 at 1897.198 kb on + strand, within QEN71_RS08495 at 1897.267 kb on - strand, within QEN71_RS08495 at 1897.310 kb on - strand, within QEN71_RS08495 at 1897.336 kb on + strand, within QEN71_RS08495 at 1897.337 kb on - strand, within QEN71_RS08495 at 1897.450 kb on - strand, within QEN71_RS08495 at 1897.469 kb on + strand, within QEN71_RS08495 at 1897.560 kb on + strand, within QEN71_RS08495 at 1897.561 kb on - strand, within QEN71_RS08495 at 1897.622 kb on + strand, within QEN71_RS08495 at 1897.623 kb on - strand, within QEN71_RS08495 at 1897.645 kb on + strand at 1897.645 kb on + strand at 1897.652 kb on - strand at 1897.700 kb on + strand at 1897.928 kb on - strand, within QEN71_RS08500 at 1897.928 kb on - strand, within QEN71_RS08500 at 1897.940 kb on + strand, within QEN71_RS08500 at 1897.941 kb on - strand, within QEN71_RS08500 at 1898.355 kb on + strand, within QEN71_RS08500 at 1898.355 kb on + strand, within QEN71_RS08500 at 1898.356 kb on - strand, within QEN71_RS08500 at 1898.399 kb on + strand, within QEN71_RS08500 at 1898.400 kb on - strand, within QEN71_RS08500 at 1898.400 kb on - strand, within QEN71_RS08500 at 1898.672 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_noSalt with Cobalt chloride 0.25 mM remove 1,895,797 - QEN71_RS08485 0.89 -2.4 1,895,821 + -0.3 1,895,856 + +0.4 1,895,857 - +0.8 1,895,936 - +0.2 1,896,315 + QEN71_RS08490 0.51 -0.2 1,896,315 + QEN71_RS08490 0.51 +0.0 1,896,316 - QEN71_RS08490 0.51 +0.0 1,896,372 - QEN71_RS08490 0.59 -1.8 1,896,372 - QEN71_RS08490 0.59 +0.3 1,896,376 - QEN71_RS08490 0.60 -0.4 1,896,383 + QEN71_RS08490 0.61 -1.6 1,896,383 + QEN71_RS08490 0.61 -0.8 1,896,426 - QEN71_RS08490 0.67 -2.8 1,896,429 + QEN71_RS08490 0.68 +0.5 1,896,429 + QEN71_RS08490 0.68 -0.7 1,896,429 + QEN71_RS08490 0.68 -0.8 1,896,429 + QEN71_RS08490 0.68 +0.9 1,896,429 + QEN71_RS08490 0.68 -1.7 1,896,429 + QEN71_RS08490 0.68 +0.2 1,896,430 - QEN71_RS08490 0.68 +0.2 1,896,787 + -0.1 1,896,788 - +0.6 1,896,821 + -1.3 1,896,984 + QEN71_RS08495 0.23 -1.1 1,896,985 - QEN71_RS08495 0.23 -1.0 1,896,985 - QEN71_RS08495 0.23 -0.7 1,896,985 - QEN71_RS08495 0.23 +0.4 1,896,985 - QEN71_RS08495 0.23 -0.2 1,897,067 - QEN71_RS08495 0.31 -0.2 1,897,144 + QEN71_RS08495 0.39 -1.7 1,897,198 + QEN71_RS08495 0.45 -0.1 1,897,267 - QEN71_RS08495 0.52 -0.3 1,897,310 - QEN71_RS08495 0.56 -0.1 1,897,336 + QEN71_RS08495 0.59 +0.6 1,897,337 - QEN71_RS08495 0.59 +0.7 1,897,450 - QEN71_RS08495 0.71 +0.4 1,897,469 + QEN71_RS08495 0.72 -1.6 1,897,560 + QEN71_RS08495 0.82 -0.5 1,897,561 - QEN71_RS08495 0.82 -0.5 1,897,622 + QEN71_RS08495 0.88 -0.8 1,897,623 - QEN71_RS08495 0.88 -1.5 1,897,645 + +0.3 1,897,645 + -0.1 1,897,652 - -0.3 1,897,700 + +0.9 1,897,928 - QEN71_RS08500 0.27 -0.1 1,897,928 - QEN71_RS08500 0.27 -0.3 1,897,940 + QEN71_RS08500 0.29 -0.7 1,897,941 - QEN71_RS08500 0.29 +0.0 1,898,355 + QEN71_RS08500 0.80 -0.4 1,898,355 + QEN71_RS08500 0.80 -0.4 1,898,356 - QEN71_RS08500 0.80 -1.5 1,898,399 + QEN71_RS08500 0.85 -0.3 1,898,400 - QEN71_RS08500 0.85 -0.1 1,898,400 - QEN71_RS08500 0.85 -2.0 1,898,672 - -1.1
Or see this region's nucleotide sequence