Experiment: Psabiae_ML3b with MT80
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt QEN71_RS03290 and QEN71_RS03295 are separated by 114 nucleotides QEN71_RS03295 and QEN71_RS03300 are separated by 24 nucleotides QEN71_RS03300 and QEN71_RS03305 are separated by 39 nucleotides
QEN71_RS03290: QEN71_RS03290 - bifunctional 3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP cyclohydrolase II, at 729,612 to 730,748
_RS03290
QEN71_RS03295: QEN71_RS03295 - riboflavin synthase, at 730,863 to 731,477
_RS03295
QEN71_RS03300: QEN71_RS03300 - bifunctional diaminohydroxyphosphoribosylaminopyrimidine deaminase/5-amino-6-(5-phosphoribosylamino)uracil reductase RibD, at 731,502 to 732,623
_RS03300
QEN71_RS03305: QEN71_RS03305 - glutamate-1-semialdehyde 2,1-aminomutase, at 732,663 to 733,943
_RS03305
Position (kb) 731
732
733 Strain fitness (log2 ratio) -1
0
1
2 at 730.508 kb on - strand, within QEN71_RS03290 at 730.508 kb on - strand, within QEN71_RS03290 at 730.780 kb on + strand at 730.781 kb on - strand at 730.819 kb on + strand at 730.819 kb on + strand at 730.819 kb on + strand at 730.819 kb on + strand at 730.819 kb on + strand at 730.819 kb on + strand at 730.819 kb on + strand at 730.819 kb on + strand at 730.819 kb on + strand at 730.820 kb on - strand at 730.820 kb on - strand at 730.820 kb on - strand at 730.820 kb on - strand at 730.820 kb on - strand at 730.820 kb on - strand at 730.820 kb on - strand at 730.820 kb on - strand at 730.820 kb on - strand at 730.820 kb on - strand at 730.820 kb on - strand at 730.820 kb on - strand at 730.820 kb on - strand at 730.820 kb on - strand at 730.820 kb on - strand at 730.820 kb on - strand at 730.820 kb on - strand at 730.864 kb on + strand at 730.864 kb on + strand at 730.864 kb on + strand at 730.865 kb on - strand at 730.865 kb on - strand at 731.491 kb on - strand at 731.491 kb on - strand at 731.491 kb on - strand at 731.491 kb on - strand at 731.491 kb on - strand at 731.491 kb on - strand at 732.644 kb on - strand at 732.644 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Psabiae_ML3b with MT80 remove 730,508 - QEN71_RS03290 0.79 +0.2 730,508 - QEN71_RS03290 0.79 -0.2 730,780 + -0.1 730,781 - -1.4 730,819 + -0.6 730,819 + +0.3 730,819 + +1.4 730,819 + +1.4 730,819 + -0.2 730,819 + +0.2 730,819 + -0.3 730,819 + +0.0 730,819 + +0.7 730,820 - -0.8 730,820 - -0.7 730,820 - +0.5 730,820 - +0.3 730,820 - +0.2 730,820 - -0.6 730,820 - -0.6 730,820 - -0.4 730,820 - -0.9 730,820 - -0.2 730,820 - +1.3 730,820 - +0.3 730,820 - -0.2 730,820 - +1.2 730,820 - -1.0 730,820 - +2.1 730,820 - -1.5 730,864 + +0.4 730,864 + -0.5 730,864 + -0.9 730,865 - -0.5 730,865 - -0.0 731,491 - -0.2 731,491 - -0.4 731,491 - -0.6 731,491 - -1.0 731,491 - +1.2 731,491 - +0.0 732,644 - -1.0 732,644 - -0.4
Or see this region's nucleotide sequence