Experiment: Filtered_supernatant_MT81_in_LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt QEN71_RS34090 and QEN71_RS34095 overlap by 26 nucleotides QEN71_RS34095 and QEN71_RS34100 are separated by 7 nucleotides QEN71_RS34100 and QEN71_RS34105 are separated by 23 nucleotides
QEN71_RS34090: QEN71_RS34090 - methenyltetrahydromethanopterin cyclohydrolase, at 963,774 to 964,802
_RS34090
QEN71_RS34095: QEN71_RS34095 - ATP-grasp domain-containing protein, at 964,777 to 965,961
_RS34095
QEN71_RS34100: QEN71_RS34100 - methylenetetrahydromethanopterin dehydrogenase, at 965,969 to 966,889
_RS34100
QEN71_RS34105: QEN71_RS34105 - beta-ribofuranosylaminobenzene 5'-phosphate synthase, at 966,913 to 967,932
_RS34105
Position (kb)
964
965
966 Strain fitness (log2 ratio)
-1
0
1 at 963.902 kb on - strand, within QEN71_RS34090 at 963.902 kb on - strand, within QEN71_RS34090 at 965.005 kb on + strand, within QEN71_RS34095 at 965.058 kb on + strand, within QEN71_RS34095 at 965.574 kb on + strand, within QEN71_RS34095 at 965.575 kb on - strand, within QEN71_RS34095 at 966.043 kb on - strand at 966.043 kb on - strand at 966.096 kb on + strand, within QEN71_RS34100 at 966.096 kb on + strand, within QEN71_RS34100 at 966.097 kb on - strand, within QEN71_RS34100 at 966.722 kb on - strand, within QEN71_RS34100 at 966.901 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Filtered_supernatant_MT81_in_LB remove 963,902 - QEN71_RS34090 0.12 -0.6 963,902 - QEN71_RS34090 0.12 +0.3 965,005 + QEN71_RS34095 0.19 -0.6 965,058 + QEN71_RS34095 0.24 -1.0 965,574 + QEN71_RS34095 0.67 -0.1 965,575 - QEN71_RS34095 0.67 -0.6 966,043 - -0.5 966,043 - -0.2 966,096 + QEN71_RS34100 0.14 +0.1 966,096 + QEN71_RS34100 0.14 +0.0 966,097 - QEN71_RS34100 0.14 -1.3 966,722 - QEN71_RS34100 0.82 -0.8 966,901 - -1.0
Or see this region's nucleotide sequence