Experiment: D,L-Malic Acid (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt QEN71_RS32000 and QEN71_RS32005 are separated by 44 nucleotides QEN71_RS32005 and QEN71_RS32010 are separated by 129 nucleotides
QEN71_RS32000: QEN71_RS32000 - NAD-dependent succinate-semialdehyde dehydrogenase, at 530,147 to 531,619
_RS32000
QEN71_RS32005: QEN71_RS32005 - tartrate dehydrogenase, at 531,664 to 532,743
_RS32005
QEN71_RS32010: QEN71_RS32010 - LysR substrate-binding domain-containing protein, at 532,873 to 533,790
_RS32010
Position (kb)
531
532
533 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 530.718 kb on + strand, within QEN71_RS32000 at 530.719 kb on - strand, within QEN71_RS32000 at 530.719 kb on - strand, within QEN71_RS32000 at 530.719 kb on - strand, within QEN71_RS32000 at 530.886 kb on + strand, within QEN71_RS32000 at 530.887 kb on - strand, within QEN71_RS32000 at 531.235 kb on - strand, within QEN71_RS32000 at 531.270 kb on + strand, within QEN71_RS32000 at 531.271 kb on - strand, within QEN71_RS32000 at 531.271 kb on - strand, within QEN71_RS32000 at 531.271 kb on - strand, within QEN71_RS32000 at 531.282 kb on + strand, within QEN71_RS32000 at 531.283 kb on - strand, within QEN71_RS32000 at 531.283 kb on - strand, within QEN71_RS32000 at 531.283 kb on - strand, within QEN71_RS32000 at 531.416 kb on - strand, within QEN71_RS32000 at 531.562 kb on + strand at 531.564 kb on - strand at 531.606 kb on + strand at 531.606 kb on + strand at 531.607 kb on - strand at 531.607 kb on - strand at 531.607 kb on - strand at 531.732 kb on + strand at 532.235 kb on - strand, within QEN71_RS32005 at 532.437 kb on - strand, within QEN71_RS32005 at 532.437 kb on - strand, within QEN71_RS32005 at 532.437 kb on - strand, within QEN71_RS32005 at 532.437 kb on - strand, within QEN71_RS32005 at 532.592 kb on + strand, within QEN71_RS32005 at 532.592 kb on + strand, within QEN71_RS32005 at 532.595 kb on + strand, within QEN71_RS32005 at 532.595 kb on + strand, within QEN71_RS32005 at 532.596 kb on - strand, within QEN71_RS32005 at 532.625 kb on + strand, within QEN71_RS32005 at 532.643 kb on + strand at 532.695 kb on + strand at 532.731 kb on - strand at 532.804 kb on + strand at 532.804 kb on + strand at 532.804 kb on + strand at 532.811 kb on + strand at 532.816 kb on + strand at 532.816 kb on + strand at 532.816 kb on + strand at 532.816 kb on + strand at 532.817 kb on - strand at 532.817 kb on - strand at 532.817 kb on - strand at 532.836 kb on - strand at 532.862 kb on - strand at 532.976 kb on - strand, within QEN71_RS32010 at 532.976 kb on - strand, within QEN71_RS32010 at 533.129 kb on + strand, within QEN71_RS32010 at 533.129 kb on + strand, within QEN71_RS32010 at 533.130 kb on - strand, within QEN71_RS32010 at 533.130 kb on - strand, within QEN71_RS32010 at 533.196 kb on + strand, within QEN71_RS32010 at 533.197 kb on - strand, within QEN71_RS32010 at 533.392 kb on + strand, within QEN71_RS32010 at 533.393 kb on - strand, within QEN71_RS32010 at 533.393 kb on - strand, within QEN71_RS32010 at 533.664 kb on + strand, within QEN71_RS32010 at 533.665 kb on - strand, within QEN71_RS32010
Per-strain Table
Position Strand Gene LocusTag Fraction D,L-Malic Acid (C) remove 530,718 + QEN71_RS32000 0.39 -1.4 530,719 - QEN71_RS32000 0.39 -0.5 530,719 - QEN71_RS32000 0.39 +0.0 530,719 - QEN71_RS32000 0.39 +0.9 530,886 + QEN71_RS32000 0.50 -0.1 530,887 - QEN71_RS32000 0.50 -0.7 531,235 - QEN71_RS32000 0.74 +0.2 531,270 + QEN71_RS32000 0.76 -0.5 531,271 - QEN71_RS32000 0.76 -1.2 531,271 - QEN71_RS32000 0.76 -0.1 531,271 - QEN71_RS32000 0.76 +0.2 531,282 + QEN71_RS32000 0.77 -0.3 531,283 - QEN71_RS32000 0.77 +0.5 531,283 - QEN71_RS32000 0.77 -0.1 531,283 - QEN71_RS32000 0.77 +0.0 531,416 - QEN71_RS32000 0.86 -0.1 531,562 + -1.4 531,564 - -0.7 531,606 + +1.3 531,606 + +0.3 531,607 - -0.8 531,607 - -0.9 531,607 - -0.3 531,732 + -1.4 532,235 - QEN71_RS32005 0.53 -0.9 532,437 - QEN71_RS32005 0.72 -1.0 532,437 - QEN71_RS32005 0.72 -2.1 532,437 - QEN71_RS32005 0.72 -0.3 532,437 - QEN71_RS32005 0.72 -0.2 532,592 + QEN71_RS32005 0.86 +0.3 532,592 + QEN71_RS32005 0.86 -0.2 532,595 + QEN71_RS32005 0.86 -1.6 532,595 + QEN71_RS32005 0.86 -2.6 532,596 - QEN71_RS32005 0.86 -2.3 532,625 + QEN71_RS32005 0.89 -4.0 532,643 + -0.2 532,695 + +1.7 532,731 - -0.7 532,804 + +1.8 532,804 + +0.2 532,804 + -1.0 532,811 + +0.1 532,816 + -0.6 532,816 + -1.6 532,816 + -0.7 532,816 + +0.2 532,817 - +0.3 532,817 - +2.2 532,817 - +1.2 532,836 - -0.6 532,862 - -2.0 532,976 - QEN71_RS32010 0.11 -0.2 532,976 - QEN71_RS32010 0.11 -0.5 533,129 + QEN71_RS32010 0.28 -0.4 533,129 + QEN71_RS32010 0.28 -0.1 533,130 - QEN71_RS32010 0.28 +0.4 533,130 - QEN71_RS32010 0.28 +0.2 533,196 + QEN71_RS32010 0.35 -0.3 533,197 - QEN71_RS32010 0.35 +0.9 533,392 + QEN71_RS32010 0.57 -0.8 533,393 - QEN71_RS32010 0.57 -0.8 533,393 - QEN71_RS32010 0.57 +0.6 533,664 + QEN71_RS32010 0.86 +0.5 533,665 - QEN71_RS32010 0.86 -0.5
Or see this region's nucleotide sequence