Experiment: L-Leucine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt QEN71_RS42185 and QEN71_RS42190 are separated by 56 nucleotides QEN71_RS42190 and QEN71_RS42195 are separated by 71 nucleotides QEN71_RS42195 and QEN71_RS42200 overlap by 4 nucleotides QEN71_RS42200 and QEN71_RS42205 are separated by 16 nucleotides
QEN71_RS42185: QEN71_RS42185 - nitrogenase iron-molybdenum cofactor biosynthesis protein NifN, at 520,924 to 522,273
_RS42185
QEN71_RS42190: QEN71_RS42190 - nitrogen fixation protein NifX, at 522,330 to 522,746
_RS42190
QEN71_RS42195: QEN71_RS42195 - CCE_0567 family metalloprotein, at 522,818 to 523,039
_RS42195
QEN71_RS42200: QEN71_RS42200 - ferredoxin III, nif-specific, at 523,036 to 523,338
_RS42200
QEN71_RS42205: QEN71_RS42205 - nitrogen fixation protein NifQ, at 523,355 to 523,927
_RS42205
Position (kb) 522
523
524 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 521.837 kb on - strand, within QEN71_RS42185 at 521.966 kb on + strand, within QEN71_RS42185 at 522.042 kb on + strand, within QEN71_RS42185 at 522.172 kb on - strand at 522.172 kb on - strand at 522.209 kb on + strand at 522.209 kb on + strand at 522.209 kb on + strand at 522.209 kb on + strand at 522.209 kb on + strand at 522.210 kb on - strand at 522.210 kb on - strand at 522.293 kb on - strand at 522.401 kb on + strand, within QEN71_RS42190 at 522.406 kb on - strand, within QEN71_RS42190 at 522.406 kb on - strand, within QEN71_RS42190 at 522.412 kb on + strand, within QEN71_RS42190 at 522.412 kb on + strand, within QEN71_RS42190 at 522.412 kb on + strand, within QEN71_RS42190 at 522.413 kb on - strand, within QEN71_RS42190 at 522.428 kb on + strand, within QEN71_RS42190 at 522.428 kb on + strand, within QEN71_RS42190 at 522.428 kb on + strand, within QEN71_RS42190 at 522.428 kb on + strand, within QEN71_RS42190 at 522.428 kb on + strand, within QEN71_RS42190 at 522.428 kb on + strand, within QEN71_RS42190 at 522.429 kb on - strand, within QEN71_RS42190 at 522.429 kb on - strand, within QEN71_RS42190 at 522.429 kb on - strand, within QEN71_RS42190 at 522.429 kb on - strand, within QEN71_RS42190 at 522.531 kb on + strand, within QEN71_RS42190 at 522.709 kb on - strand at 522.709 kb on - strand at 522.713 kb on + strand at 522.714 kb on - strand at 522.733 kb on + strand at 522.733 kb on + strand at 522.734 kb on - strand at 522.734 kb on - strand at 523.061 kb on + strand at 523.178 kb on + strand, within QEN71_RS42200 at 523.313 kb on - strand at 523.348 kb on + strand at 523.349 kb on - strand at 523.355 kb on - strand at 523.390 kb on - strand at 523.464 kb on + strand, within QEN71_RS42205 at 523.464 kb on + strand, within QEN71_RS42205 at 523.471 kb on + strand, within QEN71_RS42205 at 523.617 kb on + strand, within QEN71_RS42205 at 523.675 kb on + strand, within QEN71_RS42205 at 523.675 kb on + strand, within QEN71_RS42205 at 523.676 kb on - strand, within QEN71_RS42205 at 523.676 kb on - strand, within QEN71_RS42205 at 523.676 kb on - strand, within QEN71_RS42205 at 523.834 kb on + strand, within QEN71_RS42205 at 523.864 kb on + strand, within QEN71_RS42205 at 524.025 kb on + strand at 524.025 kb on + strand at 524.025 kb on + strand at 524.026 kb on - strand at 524.026 kb on - strand at 524.026 kb on - strand at 524.026 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Leucine (C) remove 521,837 - QEN71_RS42185 0.68 +0.2 521,966 + QEN71_RS42185 0.77 -0.2 522,042 + QEN71_RS42185 0.83 +0.6 522,172 - -2.0 522,172 - -0.3 522,209 + -0.1 522,209 + +1.1 522,209 + +3.1 522,209 + +0.6 522,209 + -2.4 522,210 - -0.5 522,210 - +0.2 522,293 - +0.1 522,401 + QEN71_RS42190 0.17 -0.7 522,406 - QEN71_RS42190 0.18 -1.2 522,406 - QEN71_RS42190 0.18 -0.1 522,412 + QEN71_RS42190 0.20 -1.4 522,412 + QEN71_RS42190 0.20 -0.8 522,412 + QEN71_RS42190 0.20 -0.6 522,413 - QEN71_RS42190 0.20 -1.0 522,428 + QEN71_RS42190 0.24 +0.4 522,428 + QEN71_RS42190 0.24 -0.6 522,428 + QEN71_RS42190 0.24 +0.2 522,428 + QEN71_RS42190 0.24 +1.1 522,428 + QEN71_RS42190 0.24 -1.8 522,428 + QEN71_RS42190 0.24 +0.2 522,429 - QEN71_RS42190 0.24 +0.6 522,429 - QEN71_RS42190 0.24 -1.0 522,429 - QEN71_RS42190 0.24 -0.2 522,429 - QEN71_RS42190 0.24 -0.5 522,531 + QEN71_RS42190 0.48 +0.6 522,709 - -1.6 522,709 - +0.2 522,713 + +0.2 522,714 - -0.6 522,733 + -0.1 522,733 + -0.9 522,734 - -1.2 522,734 - -0.9 523,061 + +0.1 523,178 + QEN71_RS42200 0.47 -1.0 523,313 - -1.3 523,348 + -1.2 523,349 - -0.3 523,355 - +1.4 523,390 - +0.8 523,464 + QEN71_RS42205 0.19 +1.5 523,464 + QEN71_RS42205 0.19 +1.1 523,471 + QEN71_RS42205 0.20 +0.8 523,617 + QEN71_RS42205 0.46 -0.9 523,675 + QEN71_RS42205 0.56 -2.5 523,675 + QEN71_RS42205 0.56 -1.4 523,676 - QEN71_RS42205 0.56 -0.7 523,676 - QEN71_RS42205 0.56 +1.0 523,676 - QEN71_RS42205 0.56 -0.2 523,834 + QEN71_RS42205 0.84 +2.4 523,864 + QEN71_RS42205 0.89 -0.3 524,025 + -0.3 524,025 + -2.2 524,025 + -0.0 524,026 - -1.4 524,026 - -0.9 524,026 - +0.4 524,026 - -0.2
Or see this region's nucleotide sequence