Experiment: L-Leucine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt QEN71_RS40220 and QEN71_RS40225 are separated by 26 nucleotides QEN71_RS40225 and QEN71_RS40230 are separated by 409 nucleotides
QEN71_RS40220: QEN71_RS40220 - biosynthetic-type acetolactate synthase large subunit, at 103,738 to 105,513
_RS40220
QEN71_RS40225: QEN71_RS40225 - alpha-ketoglutarate dehydrogenase, at 105,540 to 108,254
_RS40225
QEN71_RS40230: QEN71_RS40230 - amino acid ABC transporter ATP-binding protein, at 108,664 to 109,443
_RS40230
Position (kb)
105
106
107
108
109 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 104.554 kb on - strand, within QEN71_RS40220 at 104.554 kb on - strand, within QEN71_RS40220 at 104.881 kb on - strand, within QEN71_RS40220 at 104.881 kb on - strand, within QEN71_RS40220 at 104.904 kb on - strand, within QEN71_RS40220 at 104.930 kb on - strand, within QEN71_RS40220 at 104.970 kb on + strand, within QEN71_RS40220 at 104.980 kb on + strand, within QEN71_RS40220 at 104.981 kb on - strand, within QEN71_RS40220 at 104.981 kb on - strand, within QEN71_RS40220 at 105.066 kb on + strand, within QEN71_RS40220 at 105.066 kb on + strand, within QEN71_RS40220 at 105.067 kb on - strand, within QEN71_RS40220 at 105.088 kb on - strand, within QEN71_RS40220 at 105.242 kb on - strand, within QEN71_RS40220 at 105.242 kb on - strand, within QEN71_RS40220 at 105.350 kb on + strand at 105.350 kb on + strand at 105.391 kb on - strand at 105.517 kb on + strand at 105.517 kb on + strand at 105.746 kb on - strand at 105.746 kb on - strand at 105.760 kb on + strand at 105.760 kb on + strand at 105.761 kb on - strand at 105.761 kb on - strand at 105.794 kb on - strand at 105.847 kb on + strand, within QEN71_RS40225 at 105.848 kb on - strand, within QEN71_RS40225 at 105.848 kb on - strand, within QEN71_RS40225 at 106.066 kb on + strand, within QEN71_RS40225 at 106.066 kb on + strand, within QEN71_RS40225 at 106.066 kb on + strand, within QEN71_RS40225 at 106.066 kb on + strand, within QEN71_RS40225 at 106.067 kb on - strand, within QEN71_RS40225 at 106.067 kb on - strand, within QEN71_RS40225 at 106.070 kb on + strand, within QEN71_RS40225 at 106.071 kb on - strand, within QEN71_RS40225 at 106.071 kb on - strand, within QEN71_RS40225 at 106.133 kb on - strand, within QEN71_RS40225 at 106.225 kb on + strand, within QEN71_RS40225 at 106.244 kb on - strand, within QEN71_RS40225 at 106.421 kb on - strand, within QEN71_RS40225 at 106.424 kb on - strand, within QEN71_RS40225 at 106.424 kb on - strand, within QEN71_RS40225 at 106.429 kb on + strand, within QEN71_RS40225 at 106.524 kb on + strand, within QEN71_RS40225 at 106.553 kb on - strand, within QEN71_RS40225 at 106.553 kb on - strand, within QEN71_RS40225 at 106.603 kb on + strand, within QEN71_RS40225 at 106.603 kb on + strand, within QEN71_RS40225 at 106.603 kb on + strand, within QEN71_RS40225 at 106.604 kb on - strand, within QEN71_RS40225 at 106.604 kb on - strand, within QEN71_RS40225 at 106.837 kb on + strand, within QEN71_RS40225 at 106.838 kb on - strand, within QEN71_RS40225 at 106.871 kb on - strand, within QEN71_RS40225 at 106.904 kb on - strand, within QEN71_RS40225 at 107.110 kb on + strand, within QEN71_RS40225 at 107.122 kb on - strand, within QEN71_RS40225 at 107.409 kb on - strand, within QEN71_RS40225 at 107.591 kb on - strand, within QEN71_RS40225 at 107.776 kb on + strand, within QEN71_RS40225 at 107.828 kb on + strand, within QEN71_RS40225 at 107.891 kb on - strand, within QEN71_RS40225 at 108.116 kb on - strand at 108.341 kb on - strand at 108.400 kb on - strand at 108.440 kb on + strand at 108.561 kb on + strand at 108.561 kb on + strand at 108.562 kb on - strand at 108.565 kb on + strand at 108.566 kb on - strand at 108.566 kb on - strand at 108.566 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Leucine (C) remove 104,554 - QEN71_RS40220 0.46 +0.2 104,554 - QEN71_RS40220 0.46 +0.5 104,881 - QEN71_RS40220 0.64 -0.5 104,881 - QEN71_RS40220 0.64 -0.8 104,904 - QEN71_RS40220 0.66 +0.2 104,930 - QEN71_RS40220 0.67 -1.6 104,970 + QEN71_RS40220 0.69 -0.0 104,980 + QEN71_RS40220 0.70 -0.0 104,981 - QEN71_RS40220 0.70 -1.4 104,981 - QEN71_RS40220 0.70 -0.2 105,066 + QEN71_RS40220 0.75 +0.3 105,066 + QEN71_RS40220 0.75 -0.5 105,067 - QEN71_RS40220 0.75 -1.4 105,088 - QEN71_RS40220 0.76 +1.1 105,242 - QEN71_RS40220 0.85 -0.3 105,242 - QEN71_RS40220 0.85 -0.1 105,350 + -0.2 105,350 + +0.4 105,391 - -1.0 105,517 + -0.0 105,517 + +0.7 105,746 - +0.5 105,746 - +0.4 105,760 + +0.8 105,760 + +0.6 105,761 - +0.2 105,761 - -0.2 105,794 - -0.0 105,847 + QEN71_RS40225 0.11 +0.1 105,848 - QEN71_RS40225 0.11 -0.1 105,848 - QEN71_RS40225 0.11 -0.4 106,066 + QEN71_RS40225 0.19 +0.0 106,066 + QEN71_RS40225 0.19 -0.1 106,066 + QEN71_RS40225 0.19 -2.0 106,066 + QEN71_RS40225 0.19 -0.4 106,067 - QEN71_RS40225 0.19 -0.0 106,067 - QEN71_RS40225 0.19 +0.8 106,070 + QEN71_RS40225 0.20 -0.1 106,071 - QEN71_RS40225 0.20 -0.4 106,071 - QEN71_RS40225 0.20 +0.2 106,133 - QEN71_RS40225 0.22 +1.3 106,225 + QEN71_RS40225 0.25 +1.1 106,244 - QEN71_RS40225 0.26 -1.5 106,421 - QEN71_RS40225 0.32 +0.5 106,424 - QEN71_RS40225 0.33 -0.2 106,424 - QEN71_RS40225 0.33 +0.4 106,429 + QEN71_RS40225 0.33 -1.3 106,524 + QEN71_RS40225 0.36 -0.1 106,553 - QEN71_RS40225 0.37 +0.8 106,553 - QEN71_RS40225 0.37 -1.0 106,603 + QEN71_RS40225 0.39 -1.3 106,603 + QEN71_RS40225 0.39 -2.3 106,603 + QEN71_RS40225 0.39 -1.7 106,604 - QEN71_RS40225 0.39 -1.7 106,604 - QEN71_RS40225 0.39 -0.2 106,837 + QEN71_RS40225 0.48 -0.0 106,838 - QEN71_RS40225 0.48 +0.9 106,871 - QEN71_RS40225 0.49 +0.6 106,904 - QEN71_RS40225 0.50 -0.2 107,110 + QEN71_RS40225 0.58 +1.2 107,122 - QEN71_RS40225 0.58 -2.4 107,409 - QEN71_RS40225 0.69 -1.5 107,591 - QEN71_RS40225 0.76 -0.6 107,776 + QEN71_RS40225 0.82 +0.3 107,828 + QEN71_RS40225 0.84 -1.8 107,891 - QEN71_RS40225 0.87 +0.1 108,116 - -0.5 108,341 - -0.9 108,400 - -2.8 108,440 + -0.2 108,561 + -0.8 108,561 + -1.1 108,562 - +0.6 108,565 + +1.5 108,566 - -0.0 108,566 - -0.4 108,566 - -3.4
Or see this region's nucleotide sequence