Experiment: L-Leucine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt QEN71_RS03020 and QEN71_RS03025 are separated by 149 nucleotides QEN71_RS03025 and QEN71_RS03030 overlap by 1 nucleotides QEN71_RS03030 and QEN71_RS03035 are separated by 189 nucleotides
QEN71_RS03020: QEN71_RS03020 - bifunctional phosphopantothenoylcysteine decarboxylase/phosphopantothenate--cysteine ligase CoaBC, at 673,780 to 675,003
_RS03020
QEN71_RS03025: QEN71_RS03025 - signal peptidase II, at 675,153 to 675,653
_RS03025
QEN71_RS03030: QEN71_RS03030 - isoleucine--tRNA ligase, at 675,653 to 678,490
_RS03030
QEN71_RS03035: QEN71_RS03035 - bifunctional riboflavin kinase/FAD synthetase, at 678,680 to 679,672
_RS03035
Position (kb)
675
676
677
678
679 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 675.061 kb on - strand at 675.061 kb on - strand at 675.061 kb on - strand at 675.061 kb on - strand at 675.150 kb on - strand at 675.150 kb on - strand at 675.150 kb on - strand at 675.150 kb on - strand at 675.150 kb on - strand at 675.150 kb on - strand at 675.150 kb on - strand at 675.150 kb on - strand at 675.150 kb on - strand at 675.150 kb on - strand at 675.150 kb on - strand at 675.150 kb on - strand at 675.150 kb on - strand at 675.655 kb on - strand at 678.509 kb on - strand at 678.509 kb on - strand at 678.509 kb on - strand at 678.509 kb on - strand at 678.509 kb on - strand at 678.509 kb on - strand at 678.509 kb on - strand at 678.509 kb on - strand at 678.509 kb on - strand at 678.643 kb on - strand at 678.643 kb on - strand at 678.643 kb on - strand at 678.643 kb on - strand at 678.643 kb on - strand at 678.643 kb on - strand at 678.643 kb on - strand at 678.670 kb on - strand at 678.670 kb on - strand at 678.670 kb on - strand at 678.670 kb on - strand at 678.670 kb on - strand at 678.686 kb on - strand at 678.686 kb on - strand at 678.686 kb on - strand at 678.686 kb on - strand at 678.686 kb on - strand at 678.686 kb on - strand at 678.686 kb on - strand at 678.686 kb on - strand at 678.686 kb on - strand at 678.719 kb on - strand at 678.719 kb on - strand at 678.719 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Leucine (C) remove 675,061 - -0.5 675,061 - +0.3 675,061 - -0.1 675,061 - +1.5 675,150 - -0.5 675,150 - -0.1 675,150 - -0.7 675,150 - -1.0 675,150 - -0.2 675,150 - +1.5 675,150 - +1.9 675,150 - -0.1 675,150 - +0.9 675,150 - -0.5 675,150 - -3.2 675,150 - -0.1 675,150 - +1.1 675,655 - +1.1 678,509 - -0.5 678,509 - -1.1 678,509 - -0.4 678,509 - -0.9 678,509 - -0.7 678,509 - -0.1 678,509 - +0.9 678,509 - +0.6 678,509 - +1.0 678,643 - +0.9 678,643 - +0.0 678,643 - -1.7 678,643 - -1.0 678,643 - -0.4 678,643 - -0.4 678,643 - +0.1 678,670 - +0.9 678,670 - -0.4 678,670 - -0.6 678,670 - -0.3 678,670 - +1.8 678,686 - -1.6 678,686 - -0.1 678,686 - -1.1 678,686 - -0.1 678,686 - -1.7 678,686 - -0.9 678,686 - +0.4 678,686 - +0.9 678,686 - -1.6 678,719 - +1.0 678,719 - +0.9 678,719 - -1.3
Or see this region's nucleotide sequence