Experiment: D-Sorbitol (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt QEN71_RS01685 and QEN71_RS01690 are separated by 120 nucleotides QEN71_RS01690 and QEN71_RS01695 are separated by 155 nucleotides QEN71_RS01695 and QEN71_RS01700 are separated by 47 nucleotides QEN71_RS01700 and QEN71_RS01705 are separated by 51 nucleotides
QEN71_RS01685: QEN71_RS01685 - aminoacyl-tRNA hydrolase, at 371,125 to 371,727
_RS01685
QEN71_RS01690: QEN71_RS01690 - 50S ribosomal protein L25/general stress protein Ctc, at 371,848 to 372,459
_RS01690
QEN71_RS01695: QEN71_RS01695 - ribose-phosphate pyrophosphokinase, at 372,615 to 373,571
_RS01695
QEN71_RS01700: QEN71_RS01700 - tRNA-Gln, at 373,619 to 373,695
_RS01700
QEN71_RS01705: QEN71_RS01705 - 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol kinase, at 373,747 to 374,628
_RS01705
Position (kb) 372
373
374 Strain fitness (log2 ratio) -2
-1
0
1
2 at 371.766 kb on + strand at 371.767 kb on - strand at 371.767 kb on - strand at 371.806 kb on - strand at 371.806 kb on - strand at 371.850 kb on - strand at 371.850 kb on - strand at 371.850 kb on - strand at 371.850 kb on - strand at 371.850 kb on - strand at 372.115 kb on - strand, within QEN71_RS01690 at 372.612 kb on - strand at 372.617 kb on - strand at 372.617 kb on - strand at 372.617 kb on - strand at 372.617 kb on - strand at 372.617 kb on - strand at 372.617 kb on - strand at 372.617 kb on - strand at 372.617 kb on - strand at 373.749 kb on - strand at 373.749 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction D-Sorbitol (C) remove 371,766 + -2.1 371,767 - +0.0 371,767 - -0.6 371,806 - -0.7 371,806 - +0.6 371,850 - -0.2 371,850 - -1.0 371,850 - -0.4 371,850 - -0.2 371,850 - -0.4 372,115 - QEN71_RS01690 0.44 +0.0 372,612 - -0.4 372,617 - +0.3 372,617 - -1.3 372,617 - +0.9 372,617 - -0.5 372,617 - +0.6 372,617 - -1.4 372,617 - +0.8 372,617 - +1.3 373,749 - +1.8 373,749 - +0.3
Or see this region's nucleotide sequence