Experiment: Mixed culture with Prochlorococcus marinus str. MIT9313; Experiment B, time point 3
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MIT1002_00225 and MIT1002_00226 overlap by 8 nucleotides MIT1002_00226 and MIT1002_00227 overlap by 8 nucleotides MIT1002_00227 and MIT1002_00228 are separated by 88 nucleotides MIT1002_00228 and MIT1002_00229 are separated by 65 nucleotides
MIT1002_00225: MIT1002_00225 - putative protein involved in outer membrane biogenesis, at 248,385 to 252,380
_00225
MIT1002_00226: MIT1002_00226 - (R)-stereoselective amidase, at 252,373 to 253,191
_00226
MIT1002_00227: MIT1002_00227 - protease TldD, at 253,184 to 254,626
_00227
MIT1002_00228: MIT1002_00228 - hypothetical protein, at 254,715 to 255,143
_00228
MIT1002_00229: MIT1002_00229 - x96 protein, at 255,209 to 255,766
_00229
Position (kb) 253
254
255 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 252.185 kb on - strand at 252.185 kb on - strand at 252.204 kb on + strand at 252.229 kb on + strand at 252.273 kb on + strand at 252.281 kb on - strand at 252.283 kb on - strand at 252.311 kb on - strand at 252.352 kb on + strand at 252.353 kb on + strand at 252.401 kb on + strand at 252.409 kb on - strand at 252.489 kb on - strand, within MIT1002_00226 at 252.526 kb on + strand, within MIT1002_00226 at 252.662 kb on - strand, within MIT1002_00226 at 252.830 kb on - strand, within MIT1002_00226 at 252.837 kb on + strand, within MIT1002_00226 at 252.837 kb on + strand, within MIT1002_00226 at 252.837 kb on + strand, within MIT1002_00226 at 252.845 kb on - strand, within MIT1002_00226 at 252.852 kb on - strand, within MIT1002_00226 at 252.852 kb on - strand, within MIT1002_00226 at 252.852 kb on - strand, within MIT1002_00226 at 252.864 kb on + strand, within MIT1002_00226 at 252.908 kb on + strand, within MIT1002_00226 at 252.908 kb on + strand, within MIT1002_00226 at 252.908 kb on + strand, within MIT1002_00226 at 252.908 kb on + strand, within MIT1002_00226 at 252.908 kb on + strand, within MIT1002_00226 at 252.916 kb on - strand, within MIT1002_00226 at 252.916 kb on - strand, within MIT1002_00226 at 252.916 kb on - strand, within MIT1002_00226 at 252.963 kb on - strand, within MIT1002_00226 at 253.040 kb on + strand, within MIT1002_00226 at 253.048 kb on - strand, within MIT1002_00226 at 253.068 kb on - strand, within MIT1002_00226 at 253.223 kb on + strand at 253.231 kb on - strand at 253.275 kb on + strand at 253.374 kb on + strand, within MIT1002_00227 at 253.374 kb on + strand, within MIT1002_00227 at 253.374 kb on + strand, within MIT1002_00227 at 253.374 kb on + strand, within MIT1002_00227 at 253.382 kb on - strand, within MIT1002_00227 at 253.382 kb on - strand, within MIT1002_00227 at 253.481 kb on - strand, within MIT1002_00227 at 253.491 kb on + strand, within MIT1002_00227 at 253.526 kb on + strand, within MIT1002_00227 at 253.700 kb on + strand, within MIT1002_00227 at 253.704 kb on - strand, within MIT1002_00227 at 253.709 kb on + strand, within MIT1002_00227 at 253.740 kb on + strand, within MIT1002_00227 at 253.748 kb on - strand, within MIT1002_00227 at 253.899 kb on - strand, within MIT1002_00227 at 253.926 kb on - strand, within MIT1002_00227 at 253.942 kb on + strand, within MIT1002_00227 at 253.944 kb on - strand, within MIT1002_00227 at 253.950 kb on - strand, within MIT1002_00227 at 253.953 kb on + strand, within MIT1002_00227 at 253.961 kb on - strand, within MIT1002_00227 at 253.961 kb on - strand, within MIT1002_00227 at 253.961 kb on - strand, within MIT1002_00227 at 254.039 kb on - strand, within MIT1002_00227 at 254.172 kb on + strand, within MIT1002_00227 at 254.180 kb on - strand, within MIT1002_00227 at 254.180 kb on - strand, within MIT1002_00227 at 254.190 kb on - strand, within MIT1002_00227 at 254.200 kb on + strand, within MIT1002_00227 at 254.228 kb on - strand, within MIT1002_00227 at 254.229 kb on + strand, within MIT1002_00227 at 254.249 kb on + strand, within MIT1002_00227 at 254.253 kb on + strand, within MIT1002_00227 at 254.269 kb on + strand, within MIT1002_00227 at 254.299 kb on - strand, within MIT1002_00227 at 254.451 kb on + strand, within MIT1002_00227 at 254.451 kb on - strand, within MIT1002_00227 at 254.459 kb on - strand, within MIT1002_00227 at 254.464 kb on - strand, within MIT1002_00227 at 254.472 kb on + strand, within MIT1002_00227 at 254.472 kb on + strand, within MIT1002_00227 at 254.472 kb on + strand, within MIT1002_00227 at 254.472 kb on + strand, within MIT1002_00227 at 254.480 kb on - strand, within MIT1002_00227 at 254.480 kb on - strand, within MIT1002_00227 at 254.480 kb on - strand, within MIT1002_00227 at 254.480 kb on - strand, within MIT1002_00227 at 254.480 kb on - strand, within MIT1002_00227 at 254.509 kb on + strand at 254.521 kb on + strand at 254.542 kb on - strand at 254.569 kb on + strand at 254.571 kb on + strand at 254.571 kb on + strand at 254.577 kb on - strand at 254.578 kb on - strand at 254.579 kb on - strand at 254.584 kb on - strand at 254.584 kb on - strand at 254.589 kb on - strand at 254.621 kb on + strand at 254.621 kb on + strand at 254.621 kb on + strand at 254.621 kb on + strand at 254.621 kb on + strand at 254.629 kb on - strand at 254.639 kb on - strand at 254.675 kb on + strand at 254.676 kb on - strand at 254.701 kb on + strand at 254.701 kb on + strand at 254.709 kb on - strand at 254.709 kb on - strand at 254.709 kb on - strand at 254.750 kb on - strand at 254.794 kb on - strand, within MIT1002_00228 at 254.837 kb on - strand, within MIT1002_00228 at 254.867 kb on + strand, within MIT1002_00228 at 254.872 kb on + strand, within MIT1002_00228 at 254.880 kb on - strand, within MIT1002_00228 at 254.897 kb on - strand, within MIT1002_00228 at 254.902 kb on - strand, within MIT1002_00228 at 254.912 kb on - strand, within MIT1002_00228 at 254.912 kb on - strand, within MIT1002_00228 at 254.936 kb on + strand, within MIT1002_00228 at 254.936 kb on + strand, within MIT1002_00228 at 254.936 kb on + strand, within MIT1002_00228 at 254.936 kb on + strand, within MIT1002_00228 at 254.936 kb on + strand, within MIT1002_00228 at 254.936 kb on + strand, within MIT1002_00228 at 254.944 kb on - strand, within MIT1002_00228 at 254.944 kb on - strand, within MIT1002_00228 at 254.944 kb on - strand, within MIT1002_00228 at 254.944 kb on - strand, within MIT1002_00228 at 254.944 kb on - strand, within MIT1002_00228 at 254.944 kb on - strand, within MIT1002_00228 at 254.944 kb on - strand, within MIT1002_00228 at 254.944 kb on - strand, within MIT1002_00228 at 254.944 kb on - strand, within MIT1002_00228 at 254.944 kb on - strand, within MIT1002_00228 at 254.968 kb on + strand, within MIT1002_00228 at 255.018 kb on + strand, within MIT1002_00228 at 255.118 kb on + strand at 255.118 kb on + strand at 255.118 kb on + strand at 255.126 kb on - strand at 255.191 kb on - strand at 255.207 kb on + strand at 255.330 kb on + strand, within MIT1002_00229 at 255.338 kb on - strand, within MIT1002_00229 at 255.366 kb on + strand, within MIT1002_00229 at 255.418 kb on - strand, within MIT1002_00229 at 255.450 kb on - strand, within MIT1002_00229 at 255.472 kb on + strand, within MIT1002_00229 at 255.480 kb on - strand, within MIT1002_00229 at 255.480 kb on - strand, within MIT1002_00229 at 255.488 kb on + strand, within MIT1002_00229 at 255.489 kb on + strand, within MIT1002_00229
Per-strain Table
Position Strand Gene LocusTag Fraction Mixed culture with Prochlorococcus marinus str. MIT9313; Experiment B, time point 3 remove 252,185 - -2.4 252,185 - -1.7 252,204 + -1.3 252,229 + +0.2 252,273 + -0.5 252,281 - -0.6 252,283 - -0.6 252,311 - +0.3 252,352 + -1.1 252,353 + +1.2 252,401 + -1.6 252,409 - -1.8 252,489 - MIT1002_00226 0.14 -1.6 252,526 + MIT1002_00226 0.19 -2.3 252,662 - MIT1002_00226 0.35 -0.9 252,830 - MIT1002_00226 0.56 -2.6 252,837 + MIT1002_00226 0.57 -1.0 252,837 + MIT1002_00226 0.57 -4.0 252,837 + MIT1002_00226 0.57 -1.2 252,845 - MIT1002_00226 0.58 -0.5 252,852 - MIT1002_00226 0.58 -0.1 252,852 - MIT1002_00226 0.58 -1.6 252,852 - MIT1002_00226 0.58 +0.5 252,864 + MIT1002_00226 0.60 -3.7 252,908 + MIT1002_00226 0.65 -4.1 252,908 + MIT1002_00226 0.65 -2.8 252,908 + MIT1002_00226 0.65 -4.2 252,908 + MIT1002_00226 0.65 -2.2 252,908 + MIT1002_00226 0.65 -1.6 252,916 - MIT1002_00226 0.66 -0.4 252,916 - MIT1002_00226 0.66 -0.2 252,916 - MIT1002_00226 0.66 -2.4 252,963 - MIT1002_00226 0.72 -1.8 253,040 + MIT1002_00226 0.81 +0.1 253,048 - MIT1002_00226 0.82 +0.6 253,068 - MIT1002_00226 0.85 +1.5 253,223 + +0.0 253,231 - +0.4 253,275 + -0.2 253,374 + MIT1002_00227 0.13 -0.3 253,374 + MIT1002_00227 0.13 +1.0 253,374 + MIT1002_00227 0.13 -0.2 253,374 + MIT1002_00227 0.13 +1.6 253,382 - MIT1002_00227 0.14 -0.7 253,382 - MIT1002_00227 0.14 +0.6 253,481 - MIT1002_00227 0.21 -0.1 253,491 + MIT1002_00227 0.21 -2.0 253,526 + MIT1002_00227 0.24 -0.1 253,700 + MIT1002_00227 0.36 +1.0 253,704 - MIT1002_00227 0.36 -4.2 253,709 + MIT1002_00227 0.36 +0.2 253,740 + MIT1002_00227 0.39 +0.5 253,748 - MIT1002_00227 0.39 +0.2 253,899 - MIT1002_00227 0.50 +1.2 253,926 - MIT1002_00227 0.51 -0.3 253,942 + MIT1002_00227 0.53 -0.4 253,944 - MIT1002_00227 0.53 +1.0 253,950 - MIT1002_00227 0.53 +1.2 253,953 + MIT1002_00227 0.53 -0.1 253,961 - MIT1002_00227 0.54 +0.2 253,961 - MIT1002_00227 0.54 -0.2 253,961 - MIT1002_00227 0.54 +0.4 254,039 - MIT1002_00227 0.59 +0.3 254,172 + MIT1002_00227 0.68 +0.7 254,180 - MIT1002_00227 0.69 +0.9 254,180 - MIT1002_00227 0.69 +0.6 254,190 - MIT1002_00227 0.70 +1.3 254,200 + MIT1002_00227 0.70 +0.4 254,228 - MIT1002_00227 0.72 +1.2 254,229 + MIT1002_00227 0.72 +1.8 254,249 + MIT1002_00227 0.74 -1.2 254,253 + MIT1002_00227 0.74 -1.2 254,269 + MIT1002_00227 0.75 +0.6 254,299 - MIT1002_00227 0.77 +0.2 254,451 + MIT1002_00227 0.88 +0.4 254,451 - MIT1002_00227 0.88 +0.5 254,459 - MIT1002_00227 0.88 -1.3 254,464 - MIT1002_00227 0.89 -0.2 254,472 + MIT1002_00227 0.89 +0.7 254,472 + MIT1002_00227 0.89 +0.6 254,472 + MIT1002_00227 0.89 +1.0 254,472 + MIT1002_00227 0.89 +0.6 254,480 - MIT1002_00227 0.90 +2.2 254,480 - MIT1002_00227 0.90 +0.7 254,480 - MIT1002_00227 0.90 +1.5 254,480 - MIT1002_00227 0.90 -0.2 254,480 - MIT1002_00227 0.90 -0.1 254,509 + +0.9 254,521 + +1.2 254,542 - +1.0 254,569 + +2.2 254,571 + +0.8 254,571 + +1.4 254,577 - +2.1 254,578 - +0.9 254,579 - -0.9 254,584 - +1.0 254,584 - +0.9 254,589 - +2.5 254,621 + +0.2 254,621 + -0.4 254,621 + -0.2 254,621 + +0.5 254,621 + +0.7 254,629 - -0.4 254,639 - -2.9 254,675 + +0.8 254,676 - +0.2 254,701 + -0.8 254,701 + -1.4 254,709 - -0.1 254,709 - +0.1 254,709 - +0.6 254,750 - -2.7 254,794 - MIT1002_00228 0.18 -1.0 254,837 - MIT1002_00228 0.28 -1.1 254,867 + MIT1002_00228 0.35 +0.0 254,872 + MIT1002_00228 0.37 +0.5 254,880 - MIT1002_00228 0.38 -1.5 254,897 - MIT1002_00228 0.42 +0.0 254,902 - MIT1002_00228 0.44 +1.2 254,912 - MIT1002_00228 0.46 +0.5 254,912 - MIT1002_00228 0.46 +0.7 254,936 + MIT1002_00228 0.52 -2.0 254,936 + MIT1002_00228 0.52 +0.0 254,936 + MIT1002_00228 0.52 +2.0 254,936 + MIT1002_00228 0.52 -0.5 254,936 + MIT1002_00228 0.52 -0.3 254,936 + MIT1002_00228 0.52 +0.4 254,944 - MIT1002_00228 0.53 -0.0 254,944 - MIT1002_00228 0.53 -0.5 254,944 - MIT1002_00228 0.53 +0.0 254,944 - MIT1002_00228 0.53 +0.0 254,944 - MIT1002_00228 0.53 -0.3 254,944 - MIT1002_00228 0.53 +0.3 254,944 - MIT1002_00228 0.53 -0.2 254,944 - MIT1002_00228 0.53 -0.2 254,944 - MIT1002_00228 0.53 +0.8 254,944 - MIT1002_00228 0.53 -1.3 254,968 + MIT1002_00228 0.59 -1.7 255,018 + MIT1002_00228 0.71 +0.4 255,118 + -1.2 255,118 + +0.0 255,118 + +0.6 255,126 - -3.0 255,191 - -0.2 255,207 + +0.1 255,330 + MIT1002_00229 0.22 +1.2 255,338 - MIT1002_00229 0.23 +0.9 255,366 + MIT1002_00229 0.28 +0.4 255,418 - MIT1002_00229 0.37 +0.4 255,450 - MIT1002_00229 0.43 -1.0 255,472 + MIT1002_00229 0.47 +0.2 255,480 - MIT1002_00229 0.49 +0.7 255,480 - MIT1002_00229 0.49 -0.2 255,488 + MIT1002_00229 0.50 -0.8 255,489 + MIT1002_00229 0.50 +1.7
Or see this region's nucleotide sequence