Experiment: Mixed culture with Prochlorococcus marinus str. MIT9313; Experiment B, time point 3
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MIT1002_00224 and MIT1002_00225 are separated by 26 nucleotides MIT1002_00225 and MIT1002_00226 overlap by 8 nucleotides MIT1002_00226 and MIT1002_00227 overlap by 8 nucleotides
MIT1002_00224: MIT1002_00224 - Ribonuclease G, at 246,892 to 248,358
_00224
MIT1002_00225: MIT1002_00225 - putative protein involved in outer membrane biogenesis, at 248,385 to 252,380
_00225
MIT1002_00226: MIT1002_00226 - (R)-stereoselective amidase, at 252,373 to 253,191
_00226
MIT1002_00227: MIT1002_00227 - protease TldD, at 253,184 to 254,626
_00227
Position (kb) 248
249
250
251
252
253 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 247.562 kb on + strand, within MIT1002_00224 at 247.800 kb on + strand, within MIT1002_00224 at 247.822 kb on - strand, within MIT1002_00224 at 247.966 kb on + strand, within MIT1002_00224 at 247.976 kb on + strand, within MIT1002_00224 at 247.976 kb on + strand, within MIT1002_00224 at 247.976 kb on + strand, within MIT1002_00224 at 247.984 kb on - strand, within MIT1002_00224 at 247.984 kb on - strand, within MIT1002_00224 at 247.984 kb on - strand, within MIT1002_00224 at 248.024 kb on + strand, within MIT1002_00224 at 248.076 kb on - strand, within MIT1002_00224 at 248.091 kb on + strand, within MIT1002_00224 at 248.116 kb on + strand, within MIT1002_00224 at 248.116 kb on + strand, within MIT1002_00224 at 248.136 kb on - strand, within MIT1002_00224 at 248.247 kb on - strand at 248.368 kb on - strand at 248.469 kb on + strand at 248.487 kb on - strand at 248.520 kb on - strand at 248.551 kb on + strand at 248.589 kb on - strand at 248.610 kb on + strand at 248.680 kb on - strand at 248.739 kb on - strand at 248.739 kb on - strand at 248.741 kb on + strand at 248.746 kb on + strand at 248.814 kb on + strand, within MIT1002_00225 at 248.815 kb on + strand, within MIT1002_00225 at 248.823 kb on - strand, within MIT1002_00225 at 248.840 kb on + strand, within MIT1002_00225 at 248.883 kb on - strand, within MIT1002_00225 at 248.951 kb on + strand, within MIT1002_00225 at 248.959 kb on - strand, within MIT1002_00225 at 248.963 kb on + strand, within MIT1002_00225 at 248.963 kb on + strand, within MIT1002_00225 at 248.963 kb on + strand, within MIT1002_00225 at 248.971 kb on - strand, within MIT1002_00225 at 248.971 kb on - strand, within MIT1002_00225 at 248.976 kb on + strand, within MIT1002_00225 at 249.040 kb on - strand, within MIT1002_00225 at 249.063 kb on - strand, within MIT1002_00225 at 249.065 kb on + strand, within MIT1002_00225 at 249.117 kb on + strand, within MIT1002_00225 at 249.172 kb on + strand, within MIT1002_00225 at 249.189 kb on - strand, within MIT1002_00225 at 249.284 kb on - strand, within MIT1002_00225 at 249.344 kb on + strand, within MIT1002_00225 at 249.344 kb on + strand, within MIT1002_00225 at 249.352 kb on - strand, within MIT1002_00225 at 249.352 kb on - strand, within MIT1002_00225 at 249.352 kb on - strand, within MIT1002_00225 at 249.352 kb on - strand, within MIT1002_00225 at 249.352 kb on - strand, within MIT1002_00225 at 249.359 kb on + strand, within MIT1002_00225 at 249.375 kb on - strand, within MIT1002_00225 at 249.390 kb on + strand, within MIT1002_00225 at 249.392 kb on + strand, within MIT1002_00225 at 249.400 kb on + strand, within MIT1002_00225 at 249.400 kb on + strand, within MIT1002_00225 at 249.481 kb on + strand, within MIT1002_00225 at 249.481 kb on + strand, within MIT1002_00225 at 249.489 kb on - strand, within MIT1002_00225 at 249.524 kb on + strand, within MIT1002_00225 at 249.532 kb on - strand, within MIT1002_00225 at 249.536 kb on + strand, within MIT1002_00225 at 249.536 kb on + strand, within MIT1002_00225 at 249.544 kb on - strand, within MIT1002_00225 at 249.714 kb on - strand, within MIT1002_00225 at 249.739 kb on + strand, within MIT1002_00225 at 249.772 kb on + strand, within MIT1002_00225 at 249.795 kb on - strand, within MIT1002_00225 at 249.811 kb on + strand, within MIT1002_00225 at 249.852 kb on + strand, within MIT1002_00225 at 249.860 kb on - strand, within MIT1002_00225 at 249.860 kb on - strand, within MIT1002_00225 at 249.932 kb on + strand, within MIT1002_00225 at 250.066 kb on + strand, within MIT1002_00225 at 250.100 kb on - strand, within MIT1002_00225 at 250.102 kb on + strand, within MIT1002_00225 at 250.173 kb on - strand, within MIT1002_00225 at 250.261 kb on + strand, within MIT1002_00225 at 250.329 kb on + strand, within MIT1002_00225 at 250.387 kb on - strand, within MIT1002_00225 at 250.489 kb on - strand, within MIT1002_00225 at 250.530 kb on - strand, within MIT1002_00225 at 250.573 kb on + strand, within MIT1002_00225 at 250.640 kb on + strand, within MIT1002_00225 at 250.648 kb on - strand, within MIT1002_00225 at 250.654 kb on + strand, within MIT1002_00225 at 250.694 kb on + strand, within MIT1002_00225 at 250.801 kb on + strand, within MIT1002_00225 at 250.834 kb on - strand, within MIT1002_00225 at 250.908 kb on - strand, within MIT1002_00225 at 251.016 kb on - strand, within MIT1002_00225 at 251.027 kb on + strand, within MIT1002_00225 at 251.069 kb on + strand, within MIT1002_00225 at 251.069 kb on + strand, within MIT1002_00225 at 251.208 kb on - strand, within MIT1002_00225 at 251.216 kb on + strand, within MIT1002_00225 at 251.216 kb on + strand, within MIT1002_00225 at 251.221 kb on + strand, within MIT1002_00225 at 251.221 kb on + strand, within MIT1002_00225 at 251.229 kb on - strand, within MIT1002_00225 at 251.229 kb on - strand, within MIT1002_00225 at 251.229 kb on - strand, within MIT1002_00225 at 251.229 kb on - strand, within MIT1002_00225 at 251.255 kb on - strand, within MIT1002_00225 at 251.278 kb on - strand, within MIT1002_00225 at 251.329 kb on - strand, within MIT1002_00225 at 251.403 kb on - strand, within MIT1002_00225 at 251.416 kb on + strand, within MIT1002_00225 at 251.429 kb on + strand, within MIT1002_00225 at 251.455 kb on - strand, within MIT1002_00225 at 251.460 kb on - strand, within MIT1002_00225 at 251.495 kb on + strand, within MIT1002_00225 at 251.495 kb on + strand, within MIT1002_00225 at 251.503 kb on - strand, within MIT1002_00225 at 251.503 kb on - strand, within MIT1002_00225 at 251.532 kb on + strand, within MIT1002_00225 at 251.623 kb on - strand, within MIT1002_00225 at 251.627 kb on + strand, within MIT1002_00225 at 251.673 kb on - strand, within MIT1002_00225 at 251.707 kb on + strand, within MIT1002_00225 at 251.727 kb on - strand, within MIT1002_00225 at 251.785 kb on + strand, within MIT1002_00225 at 251.816 kb on - strand, within MIT1002_00225 at 251.866 kb on + strand, within MIT1002_00225 at 251.874 kb on - strand, within MIT1002_00225 at 251.874 kb on - strand, within MIT1002_00225 at 251.903 kb on + strand, within MIT1002_00225 at 251.995 kb on - strand at 252.102 kb on + strand at 252.110 kb on - strand at 252.146 kb on - strand at 252.167 kb on - strand at 252.177 kb on + strand at 252.177 kb on + strand at 252.185 kb on - strand at 252.185 kb on - strand at 252.204 kb on + strand at 252.229 kb on + strand at 252.273 kb on + strand at 252.281 kb on - strand at 252.283 kb on - strand at 252.311 kb on - strand at 252.352 kb on + strand at 252.353 kb on + strand at 252.401 kb on + strand at 252.409 kb on - strand at 252.489 kb on - strand, within MIT1002_00226 at 252.526 kb on + strand, within MIT1002_00226 at 252.662 kb on - strand, within MIT1002_00226 at 252.830 kb on - strand, within MIT1002_00226 at 252.837 kb on + strand, within MIT1002_00226 at 252.837 kb on + strand, within MIT1002_00226 at 252.837 kb on + strand, within MIT1002_00226 at 252.845 kb on - strand, within MIT1002_00226 at 252.852 kb on - strand, within MIT1002_00226 at 252.852 kb on - strand, within MIT1002_00226 at 252.852 kb on - strand, within MIT1002_00226 at 252.864 kb on + strand, within MIT1002_00226 at 252.908 kb on + strand, within MIT1002_00226 at 252.908 kb on + strand, within MIT1002_00226 at 252.908 kb on + strand, within MIT1002_00226 at 252.908 kb on + strand, within MIT1002_00226 at 252.908 kb on + strand, within MIT1002_00226 at 252.916 kb on - strand, within MIT1002_00226 at 252.916 kb on - strand, within MIT1002_00226 at 252.916 kb on - strand, within MIT1002_00226 at 252.963 kb on - strand, within MIT1002_00226 at 253.040 kb on + strand, within MIT1002_00226 at 253.048 kb on - strand, within MIT1002_00226 at 253.068 kb on - strand, within MIT1002_00226 at 253.223 kb on + strand at 253.231 kb on - strand at 253.275 kb on + strand at 253.374 kb on + strand, within MIT1002_00227 at 253.374 kb on + strand, within MIT1002_00227 at 253.374 kb on + strand, within MIT1002_00227 at 253.374 kb on + strand, within MIT1002_00227
Per-strain Table
Position Strand Gene LocusTag Fraction Mixed culture with Prochlorococcus marinus str. MIT9313; Experiment B, time point 3 remove 247,562 + MIT1002_00224 0.46 +1.3 247,800 + MIT1002_00224 0.62 -1.3 247,822 - MIT1002_00224 0.63 -0.3 247,966 + MIT1002_00224 0.73 -2.6 247,976 + MIT1002_00224 0.74 +1.0 247,976 + MIT1002_00224 0.74 +0.6 247,976 + MIT1002_00224 0.74 -2.8 247,984 - MIT1002_00224 0.74 -3.0 247,984 - MIT1002_00224 0.74 -1.4 247,984 - MIT1002_00224 0.74 -0.6 248,024 + MIT1002_00224 0.77 -1.8 248,076 - MIT1002_00224 0.81 -3.2 248,091 + MIT1002_00224 0.82 -1.3 248,116 + MIT1002_00224 0.83 -0.3 248,116 + MIT1002_00224 0.83 -1.1 248,136 - MIT1002_00224 0.85 -1.6 248,247 - +1.2 248,368 - +0.2 248,469 + -0.2 248,487 - -2.1 248,520 - -1.3 248,551 + -1.4 248,589 - -1.1 248,610 + -1.3 248,680 - -0.4 248,739 - +0.2 248,739 - -0.2 248,741 + +0.9 248,746 + -1.0 248,814 + MIT1002_00225 0.11 -0.8 248,815 + MIT1002_00225 0.11 -1.6 248,823 - MIT1002_00225 0.11 -0.8 248,840 + MIT1002_00225 0.11 -1.1 248,883 - MIT1002_00225 0.12 -1.7 248,951 + MIT1002_00225 0.14 -2.3 248,959 - MIT1002_00225 0.14 -0.3 248,963 + MIT1002_00225 0.14 -0.8 248,963 + MIT1002_00225 0.14 -2.3 248,963 + MIT1002_00225 0.14 -2.1 248,971 - MIT1002_00225 0.15 +0.6 248,971 - MIT1002_00225 0.15 -0.1 248,976 + MIT1002_00225 0.15 -2.4 249,040 - MIT1002_00225 0.16 -2.1 249,063 - MIT1002_00225 0.17 -0.5 249,065 + MIT1002_00225 0.17 -2.2 249,117 + MIT1002_00225 0.18 -1.0 249,172 + MIT1002_00225 0.20 -1.2 249,189 - MIT1002_00225 0.20 -1.8 249,284 - MIT1002_00225 0.22 -0.8 249,344 + MIT1002_00225 0.24 -3.2 249,344 + MIT1002_00225 0.24 -1.8 249,352 - MIT1002_00225 0.24 +0.0 249,352 - MIT1002_00225 0.24 -1.5 249,352 - MIT1002_00225 0.24 -1.7 249,352 - MIT1002_00225 0.24 -2.9 249,352 - MIT1002_00225 0.24 -0.6 249,359 + MIT1002_00225 0.24 -0.2 249,375 - MIT1002_00225 0.25 -1.3 249,390 + MIT1002_00225 0.25 -1.8 249,392 + MIT1002_00225 0.25 -0.5 249,400 + MIT1002_00225 0.25 -0.2 249,400 + MIT1002_00225 0.25 -0.1 249,481 + MIT1002_00225 0.27 -2.1 249,481 + MIT1002_00225 0.27 -1.8 249,489 - MIT1002_00225 0.28 +0.4 249,524 + MIT1002_00225 0.29 -1.0 249,532 - MIT1002_00225 0.29 -1.1 249,536 + MIT1002_00225 0.29 -2.4 249,536 + MIT1002_00225 0.29 -1.2 249,544 - MIT1002_00225 0.29 -2.1 249,714 - MIT1002_00225 0.33 -1.8 249,739 + MIT1002_00225 0.34 -2.3 249,772 + MIT1002_00225 0.35 -1.5 249,795 - MIT1002_00225 0.35 -0.6 249,811 + MIT1002_00225 0.36 -2.4 249,852 + MIT1002_00225 0.37 -0.3 249,860 - MIT1002_00225 0.37 -0.8 249,860 - MIT1002_00225 0.37 +0.0 249,932 + MIT1002_00225 0.39 -0.5 250,066 + MIT1002_00225 0.42 -1.2 250,100 - MIT1002_00225 0.43 -3.6 250,102 + MIT1002_00225 0.43 -0.8 250,173 - MIT1002_00225 0.45 -1.4 250,261 + MIT1002_00225 0.47 -0.2 250,329 + MIT1002_00225 0.49 -0.9 250,387 - MIT1002_00225 0.50 -2.2 250,489 - MIT1002_00225 0.53 -2.6 250,530 - MIT1002_00225 0.54 -2.2 250,573 + MIT1002_00225 0.55 +0.1 250,640 + MIT1002_00225 0.56 -1.5 250,648 - MIT1002_00225 0.57 -1.8 250,654 + MIT1002_00225 0.57 -1.0 250,694 + MIT1002_00225 0.58 -0.3 250,801 + MIT1002_00225 0.60 -1.4 250,834 - MIT1002_00225 0.61 -1.8 250,908 - MIT1002_00225 0.63 -1.0 251,016 - MIT1002_00225 0.66 -1.3 251,027 + MIT1002_00225 0.66 -1.9 251,069 + MIT1002_00225 0.67 -1.4 251,069 + MIT1002_00225 0.67 -0.7 251,208 - MIT1002_00225 0.71 -0.9 251,216 + MIT1002_00225 0.71 -1.4 251,216 + MIT1002_00225 0.71 -1.3 251,221 + MIT1002_00225 0.71 +0.0 251,221 + MIT1002_00225 0.71 +1.3 251,229 - MIT1002_00225 0.71 -4.1 251,229 - MIT1002_00225 0.71 -2.0 251,229 - MIT1002_00225 0.71 -1.7 251,229 - MIT1002_00225 0.71 -1.5 251,255 - MIT1002_00225 0.72 -1.5 251,278 - MIT1002_00225 0.72 -0.0 251,329 - MIT1002_00225 0.74 -1.0 251,403 - MIT1002_00225 0.76 -0.4 251,416 + MIT1002_00225 0.76 -1.8 251,429 + MIT1002_00225 0.76 -1.1 251,455 - MIT1002_00225 0.77 -1.9 251,460 - MIT1002_00225 0.77 -1.8 251,495 + MIT1002_00225 0.78 -2.2 251,495 + MIT1002_00225 0.78 -0.7 251,503 - MIT1002_00225 0.78 -2.3 251,503 - MIT1002_00225 0.78 -1.2 251,532 + MIT1002_00225 0.79 -0.7 251,623 - MIT1002_00225 0.81 -1.6 251,627 + MIT1002_00225 0.81 -1.0 251,673 - MIT1002_00225 0.82 -0.8 251,707 + MIT1002_00225 0.83 +0.1 251,727 - MIT1002_00225 0.84 -0.7 251,785 + MIT1002_00225 0.85 -2.0 251,816 - MIT1002_00225 0.86 -0.1 251,866 + MIT1002_00225 0.87 -0.4 251,874 - MIT1002_00225 0.87 -2.3 251,874 - MIT1002_00225 0.87 +0.2 251,903 + MIT1002_00225 0.88 -1.8 251,995 - -0.4 252,102 + -1.6 252,110 - -1.2 252,146 - -1.1 252,167 - +1.5 252,177 + -1.2 252,177 + -1.0 252,185 - -2.4 252,185 - -1.7 252,204 + -1.3 252,229 + +0.2 252,273 + -0.5 252,281 - -0.6 252,283 - -0.6 252,311 - +0.3 252,352 + -1.1 252,353 + +1.2 252,401 + -1.6 252,409 - -1.8 252,489 - MIT1002_00226 0.14 -1.6 252,526 + MIT1002_00226 0.19 -2.3 252,662 - MIT1002_00226 0.35 -0.9 252,830 - MIT1002_00226 0.56 -2.6 252,837 + MIT1002_00226 0.57 -1.0 252,837 + MIT1002_00226 0.57 -4.0 252,837 + MIT1002_00226 0.57 -1.2 252,845 - MIT1002_00226 0.58 -0.5 252,852 - MIT1002_00226 0.58 -0.1 252,852 - MIT1002_00226 0.58 -1.6 252,852 - MIT1002_00226 0.58 +0.5 252,864 + MIT1002_00226 0.60 -3.7 252,908 + MIT1002_00226 0.65 -4.1 252,908 + MIT1002_00226 0.65 -2.8 252,908 + MIT1002_00226 0.65 -4.2 252,908 + MIT1002_00226 0.65 -2.2 252,908 + MIT1002_00226 0.65 -1.6 252,916 - MIT1002_00226 0.66 -0.4 252,916 - MIT1002_00226 0.66 -0.2 252,916 - MIT1002_00226 0.66 -2.4 252,963 - MIT1002_00226 0.72 -1.8 253,040 + MIT1002_00226 0.81 +0.1 253,048 - MIT1002_00226 0.82 +0.6 253,068 - MIT1002_00226 0.85 +1.5 253,223 + +0.0 253,231 - +0.4 253,275 + -0.2 253,374 + MIT1002_00227 0.13 -0.3 253,374 + MIT1002_00227 0.13 +1.0 253,374 + MIT1002_00227 0.13 -0.2 253,374 + MIT1002_00227 0.13 +1.6
Or see this region's nucleotide sequence