Experiment: biofilm; Day1; NLDM; Manganese (II) chloride tetrahydrate 10mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt LRK53_RS18920 and LRK53_RS18925 overlap by 4 nucleotides LRK53_RS18925 and LRK53_RS18930 overlap by 22 nucleotides LRK53_RS18930 and LRK53_RS18935 overlap by 1 nucleotides LRK53_RS18935 and LRK53_RS18940 are separated by 121 nucleotides
LRK53_RS18920: LRK53_RS18920 - S49 family peptidase, at 180,300 to 181,295
_RS18920
LRK53_RS18925: LRK53_RS18925 - hypothetical protein, at 181,292 to 181,768
_RS18925
LRK53_RS18930: LRK53_RS18930 - hypothetical protein, at 181,747 to 182,415
_RS18930
LRK53_RS18935: LRK53_RS18935 - PD-(D/E)XK nuclease family protein, at 182,415 to 183,017
_RS18935
LRK53_RS18940: LRK53_RS18940 - hypothetical protein, at 183,139 to 183,600
_RS18940
Position (kb) 181
182
183 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 180.762 kb on + strand, within LRK53_RS18920 at 180.762 kb on + strand, within LRK53_RS18920 at 180.762 kb on + strand, within LRK53_RS18920 at 180.762 kb on + strand, within LRK53_RS18920 at 180.762 kb on + strand, within LRK53_RS18920 at 180.762 kb on + strand, within LRK53_RS18920 at 180.762 kb on + strand, within LRK53_RS18920 at 180.762 kb on + strand, within LRK53_RS18920 at 180.763 kb on - strand, within LRK53_RS18920 at 180.763 kb on - strand, within LRK53_RS18920 at 180.763 kb on - strand, within LRK53_RS18920 at 180.763 kb on - strand, within LRK53_RS18920 at 180.763 kb on - strand, within LRK53_RS18920 at 180.763 kb on - strand, within LRK53_RS18920 at 180.763 kb on - strand, within LRK53_RS18920 at 180.763 kb on - strand, within LRK53_RS18920 at 180.763 kb on - strand, within LRK53_RS18920 at 180.763 kb on - strand, within LRK53_RS18920 at 180.763 kb on - strand, within LRK53_RS18920 at 180.798 kb on + strand, within LRK53_RS18920 at 180.798 kb on + strand, within LRK53_RS18920 at 180.798 kb on + strand, within LRK53_RS18920 at 180.798 kb on + strand, within LRK53_RS18920 at 180.799 kb on - strand, within LRK53_RS18920 at 180.799 kb on - strand, within LRK53_RS18920 at 180.799 kb on - strand, within LRK53_RS18920 at 180.799 kb on - strand, within LRK53_RS18920 at 180.799 kb on - strand, within LRK53_RS18920 at 180.799 kb on - strand, within LRK53_RS18920 at 180.829 kb on - strand, within LRK53_RS18920 at 180.829 kb on - strand, within LRK53_RS18920 at 180.840 kb on + strand, within LRK53_RS18920 at 180.840 kb on + strand, within LRK53_RS18920 at 180.840 kb on + strand, within LRK53_RS18920 at 180.840 kb on + strand, within LRK53_RS18920 at 180.841 kb on - strand, within LRK53_RS18920 at 180.841 kb on - strand, within LRK53_RS18920 at 180.841 kb on - strand, within LRK53_RS18920 at 180.841 kb on - strand, within LRK53_RS18920 at 180.841 kb on - strand, within LRK53_RS18920 at 180.841 kb on - strand, within LRK53_RS18920 at 180.876 kb on + strand, within LRK53_RS18920 at 180.877 kb on - strand, within LRK53_RS18920 at 180.877 kb on - strand, within LRK53_RS18920 at 181.316 kb on + strand at 181.316 kb on + strand at 181.316 kb on + strand at 181.316 kb on + strand at 181.316 kb on + strand at 181.316 kb on + strand at 181.317 kb on - strand at 181.317 kb on - strand at 181.317 kb on - strand at 181.317 kb on - strand at 181.317 kb on - strand at 181.317 kb on - strand at 181.317 kb on - strand at 181.317 kb on - strand at 181.317 kb on - strand at 181.382 kb on + strand, within LRK53_RS18925 at 181.619 kb on + strand, within LRK53_RS18925 at 181.619 kb on + strand, within LRK53_RS18925 at 181.619 kb on + strand, within LRK53_RS18925 at 181.619 kb on + strand, within LRK53_RS18925 at 181.619 kb on + strand, within LRK53_RS18925 at 181.619 kb on + strand, within LRK53_RS18925 at 181.619 kb on + strand, within LRK53_RS18925 at 181.619 kb on + strand, within LRK53_RS18925 at 181.619 kb on + strand, within LRK53_RS18925 at 181.619 kb on + strand, within LRK53_RS18925 at 181.620 kb on - strand, within LRK53_RS18925 at 181.620 kb on - strand, within LRK53_RS18925 at 181.620 kb on - strand, within LRK53_RS18925 at 181.620 kb on - strand, within LRK53_RS18925 at 181.620 kb on - strand, within LRK53_RS18925 at 181.620 kb on - strand, within LRK53_RS18925 at 181.620 kb on - strand, within LRK53_RS18925 at 181.620 kb on - strand, within LRK53_RS18925 at 181.622 kb on + strand, within LRK53_RS18925 at 181.622 kb on + strand, within LRK53_RS18925 at 181.622 kb on + strand, within LRK53_RS18925 at 181.622 kb on + strand, within LRK53_RS18925 at 181.622 kb on + strand, within LRK53_RS18925 at 181.622 kb on + strand, within LRK53_RS18925 at 181.623 kb on - strand, within LRK53_RS18925 at 181.623 kb on - strand, within LRK53_RS18925 at 181.623 kb on - strand, within LRK53_RS18925 at 181.623 kb on - strand, within LRK53_RS18925 at 181.623 kb on - strand, within LRK53_RS18925 at 181.623 kb on - strand, within LRK53_RS18925 at 181.787 kb on + strand at 181.787 kb on + strand at 181.788 kb on - strand at 181.788 kb on - strand at 181.872 kb on + strand, within LRK53_RS18930 at 181.873 kb on - strand, within LRK53_RS18930 at 182.092 kb on + strand, within LRK53_RS18930 at 182.092 kb on + strand, within LRK53_RS18930 at 182.092 kb on + strand, within LRK53_RS18930 at 182.092 kb on + strand, within LRK53_RS18930 at 182.093 kb on - strand, within LRK53_RS18930 at 182.093 kb on - strand, within LRK53_RS18930 at 182.093 kb on - strand, within LRK53_RS18930 at 182.093 kb on - strand, within LRK53_RS18930 at 182.093 kb on - strand, within LRK53_RS18930 at 182.237 kb on - strand, within LRK53_RS18930 at 182.237 kb on - strand, within LRK53_RS18930 at 182.237 kb on - strand, within LRK53_RS18930 at 182.349 kb on + strand at 182.349 kb on + strand at 182.350 kb on - strand at 182.460 kb on + strand at 182.460 kb on + strand at 182.460 kb on + strand at 182.460 kb on + strand at 182.461 kb on - strand at 182.461 kb on - strand at 182.461 kb on - strand at 182.461 kb on - strand at 182.461 kb on - strand at 182.520 kb on + strand, within LRK53_RS18935 at 182.520 kb on + strand, within LRK53_RS18935 at 182.520 kb on + strand, within LRK53_RS18935 at 182.521 kb on - strand, within LRK53_RS18935 at 182.521 kb on - strand, within LRK53_RS18935 at 182.589 kb on + strand, within LRK53_RS18935 at 182.589 kb on + strand, within LRK53_RS18935 at 182.589 kb on + strand, within LRK53_RS18935 at 182.589 kb on + strand, within LRK53_RS18935 at 182.589 kb on + strand, within LRK53_RS18935 at 182.589 kb on + strand, within LRK53_RS18935 at 182.589 kb on + strand, within LRK53_RS18935 at 182.589 kb on + strand, within LRK53_RS18935 at 182.589 kb on + strand, within LRK53_RS18935 at 182.589 kb on + strand, within LRK53_RS18935 at 182.590 kb on - strand, within LRK53_RS18935 at 182.590 kb on - strand, within LRK53_RS18935 at 182.590 kb on - strand, within LRK53_RS18935 at 182.590 kb on - strand, within LRK53_RS18935 at 182.590 kb on - strand, within LRK53_RS18935 at 182.590 kb on - strand, within LRK53_RS18935 at 182.590 kb on - strand, within LRK53_RS18935 at 182.590 kb on - strand, within LRK53_RS18935 at 182.590 kb on - strand, within LRK53_RS18935 at 182.590 kb on - strand, within LRK53_RS18935 at 182.590 kb on - strand, within LRK53_RS18935 at 182.664 kb on + strand, within LRK53_RS18935 at 182.664 kb on + strand, within LRK53_RS18935 at 182.664 kb on + strand, within LRK53_RS18935 at 182.664 kb on + strand, within LRK53_RS18935 at 182.665 kb on - strand, within LRK53_RS18935 at 182.665 kb on - strand, within LRK53_RS18935 at 182.665 kb on - strand, within LRK53_RS18935 at 182.665 kb on - strand, within LRK53_RS18935 at 182.665 kb on - strand, within LRK53_RS18935 at 182.665 kb on - strand, within LRK53_RS18935 at 182.665 kb on - strand, within LRK53_RS18935 at 182.665 kb on - strand, within LRK53_RS18935 at 182.665 kb on - strand, within LRK53_RS18935 at 182.709 kb on + strand, within LRK53_RS18935 at 182.798 kb on + strand, within LRK53_RS18935 at 182.799 kb on - strand, within LRK53_RS18935 at 182.799 kb on - strand, within LRK53_RS18935 at 182.799 kb on - strand, within LRK53_RS18935 at 182.829 kb on + strand, within LRK53_RS18935 at 182.829 kb on + strand, within LRK53_RS18935 at 182.829 kb on + strand, within LRK53_RS18935 at 182.829 kb on + strand, within LRK53_RS18935 at 182.829 kb on + strand, within LRK53_RS18935 at 182.829 kb on + strand, within LRK53_RS18935 at 182.829 kb on + strand, within LRK53_RS18935 at 182.829 kb on + strand, within LRK53_RS18935 at 182.829 kb on + strand, within LRK53_RS18935 at 182.830 kb on - strand, within LRK53_RS18935 at 182.830 kb on - strand, within LRK53_RS18935 at 182.830 kb on - strand, within LRK53_RS18935 at 182.830 kb on - strand, within LRK53_RS18935 at 182.830 kb on - strand, within LRK53_RS18935 at 182.830 kb on - strand, within LRK53_RS18935 at 182.830 kb on - strand, within LRK53_RS18935 at 182.883 kb on + strand, within LRK53_RS18935 at 182.956 kb on - strand, within LRK53_RS18935 at 183.133 kb on + strand at 183.134 kb on - strand at 183.134 kb on - strand at 183.134 kb on - strand at 183.134 kb on - strand at 183.310 kb on + strand, within LRK53_RS18940 at 183.310 kb on + strand, within LRK53_RS18940 at 183.310 kb on + strand, within LRK53_RS18940 at 183.310 kb on + strand, within LRK53_RS18940 at 183.310 kb on + strand, within LRK53_RS18940 at 183.311 kb on - strand, within LRK53_RS18940 at 183.311 kb on - strand, within LRK53_RS18940 at 183.311 kb on - strand, within LRK53_RS18940 at 183.325 kb on + strand, within LRK53_RS18940 at 183.397 kb on + strand, within LRK53_RS18940 at 183.397 kb on + strand, within LRK53_RS18940 at 183.397 kb on + strand, within LRK53_RS18940 at 183.397 kb on + strand, within LRK53_RS18940 at 183.397 kb on + strand, within LRK53_RS18940 at 183.397 kb on + strand, within LRK53_RS18940 at 183.397 kb on + strand, within LRK53_RS18940 at 183.397 kb on + strand, within LRK53_RS18940 at 183.397 kb on + strand, within LRK53_RS18940 at 183.397 kb on + strand, within LRK53_RS18940 at 183.398 kb on - strand, within LRK53_RS18940 at 183.398 kb on - strand, within LRK53_RS18940 at 183.398 kb on - strand, within LRK53_RS18940 at 183.398 kb on - strand, within LRK53_RS18940 at 183.398 kb on - strand, within LRK53_RS18940 at 183.398 kb on - strand, within LRK53_RS18940 at 183.398 kb on - strand, within LRK53_RS18940 at 183.398 kb on - strand, within LRK53_RS18940 at 183.398 kb on - strand, within LRK53_RS18940 at 183.398 kb on - strand, within LRK53_RS18940 at 183.398 kb on - strand, within LRK53_RS18940 at 183.398 kb on - strand, within LRK53_RS18940
Per-strain Table
Position Strand Gene LocusTag Fraction biofilm; Day1; NLDM; Manganese (II) chloride tetrahydrate 10mM remove 180,762 + LRK53_RS18920 0.46 +2.1 180,762 + LRK53_RS18920 0.46 -0.9 180,762 + LRK53_RS18920 0.46 +1.1 180,762 + LRK53_RS18920 0.46 -0.9 180,762 + LRK53_RS18920 0.46 -0.3 180,762 + LRK53_RS18920 0.46 -0.8 180,762 + LRK53_RS18920 0.46 -1.1 180,762 + LRK53_RS18920 0.46 -0.8 180,763 - LRK53_RS18920 0.46 -1.1 180,763 - LRK53_RS18920 0.46 +0.0 180,763 - LRK53_RS18920 0.46 -1.8 180,763 - LRK53_RS18920 0.46 -0.3 180,763 - LRK53_RS18920 0.46 -1.1 180,763 - LRK53_RS18920 0.46 -0.1 180,763 - LRK53_RS18920 0.46 -0.1 180,763 - LRK53_RS18920 0.46 -0.9 180,763 - LRK53_RS18920 0.46 -0.8 180,763 - LRK53_RS18920 0.46 -1.3 180,763 - LRK53_RS18920 0.46 -2.4 180,798 + LRK53_RS18920 0.50 -0.9 180,798 + LRK53_RS18920 0.50 -2.2 180,798 + LRK53_RS18920 0.50 -0.9 180,798 + LRK53_RS18920 0.50 -0.2 180,799 - LRK53_RS18920 0.50 +0.5 180,799 - LRK53_RS18920 0.50 -0.8 180,799 - LRK53_RS18920 0.50 -2.1 180,799 - LRK53_RS18920 0.50 -0.8 180,799 - LRK53_RS18920 0.50 -0.6 180,799 - LRK53_RS18920 0.50 +0.3 180,829 - LRK53_RS18920 0.53 -1.4 180,829 - LRK53_RS18920 0.53 -1.6 180,840 + LRK53_RS18920 0.54 +0.6 180,840 + LRK53_RS18920 0.54 -1.9 180,840 + LRK53_RS18920 0.54 -0.9 180,840 + LRK53_RS18920 0.54 -1.3 180,841 - LRK53_RS18920 0.54 -1.4 180,841 - LRK53_RS18920 0.54 -0.5 180,841 - LRK53_RS18920 0.54 -1.1 180,841 - LRK53_RS18920 0.54 -1.1 180,841 - LRK53_RS18920 0.54 -1.5 180,841 - LRK53_RS18920 0.54 +0.6 180,876 + LRK53_RS18920 0.58 +1.6 180,877 - LRK53_RS18920 0.58 -1.5 180,877 - LRK53_RS18920 0.58 +0.5 181,316 + -0.8 181,316 + +0.5 181,316 + -0.6 181,316 + +0.9 181,316 + +0.3 181,316 + -1.1 181,317 - -1.6 181,317 - -1.4 181,317 - +0.6 181,317 - -0.9 181,317 - -0.0 181,317 - -0.3 181,317 - +0.5 181,317 - +0.7 181,317 - +0.2 181,382 + LRK53_RS18925 0.19 -0.3 181,619 + LRK53_RS18925 0.69 -1.7 181,619 + LRK53_RS18925 0.69 +0.2 181,619 + LRK53_RS18925 0.69 -0.3 181,619 + LRK53_RS18925 0.69 +3.1 181,619 + LRK53_RS18925 0.69 -1.3 181,619 + LRK53_RS18925 0.69 -2.1 181,619 + LRK53_RS18925 0.69 +0.2 181,619 + LRK53_RS18925 0.69 +2.4 181,619 + LRK53_RS18925 0.69 +1.3 181,619 + LRK53_RS18925 0.69 +0.5 181,620 - LRK53_RS18925 0.69 -0.8 181,620 - LRK53_RS18925 0.69 +1.1 181,620 - LRK53_RS18925 0.69 -1.7 181,620 - LRK53_RS18925 0.69 -1.6 181,620 - LRK53_RS18925 0.69 -0.8 181,620 - LRK53_RS18925 0.69 -1.3 181,620 - LRK53_RS18925 0.69 -0.3 181,620 - LRK53_RS18925 0.69 -1.6 181,622 + LRK53_RS18925 0.69 -0.5 181,622 + LRK53_RS18925 0.69 -0.9 181,622 + LRK53_RS18925 0.69 -1.1 181,622 + LRK53_RS18925 0.69 +0.9 181,622 + LRK53_RS18925 0.69 -0.3 181,622 + LRK53_RS18925 0.69 +1.3 181,623 - LRK53_RS18925 0.69 -2.5 181,623 - LRK53_RS18925 0.69 -0.9 181,623 - LRK53_RS18925 0.69 -0.6 181,623 - LRK53_RS18925 0.69 -0.8 181,623 - LRK53_RS18925 0.69 +0.7 181,623 - LRK53_RS18925 0.69 +0.5 181,787 + -0.5 181,787 + -0.8 181,788 - +0.5 181,788 - -0.9 181,872 + LRK53_RS18930 0.19 -0.0 181,873 - LRK53_RS18930 0.19 +0.2 182,092 + LRK53_RS18930 0.52 -1.1 182,092 + LRK53_RS18930 0.52 +0.2 182,092 + LRK53_RS18930 0.52 +0.0 182,092 + LRK53_RS18930 0.52 -0.8 182,093 - LRK53_RS18930 0.52 -0.2 182,093 - LRK53_RS18930 0.52 +0.2 182,093 - LRK53_RS18930 0.52 -0.5 182,093 - LRK53_RS18930 0.52 -0.6 182,093 - LRK53_RS18930 0.52 -1.1 182,237 - LRK53_RS18930 0.73 +1.7 182,237 - LRK53_RS18930 0.73 +0.6 182,237 - LRK53_RS18930 0.73 -3.1 182,349 + -1.9 182,349 + -0.7 182,350 - -0.9 182,460 + -1.3 182,460 + -0.5 182,460 + -0.9 182,460 + -1.8 182,461 - -0.5 182,461 - -2.1 182,461 - -0.2 182,461 - -1.1 182,461 - -0.2 182,520 + LRK53_RS18935 0.17 -0.8 182,520 + LRK53_RS18935 0.17 -0.5 182,520 + LRK53_RS18935 0.17 -0.9 182,521 - LRK53_RS18935 0.18 -0.9 182,521 - LRK53_RS18935 0.18 -1.8 182,589 + LRK53_RS18935 0.29 -0.7 182,589 + LRK53_RS18935 0.29 -0.8 182,589 + LRK53_RS18935 0.29 -0.8 182,589 + LRK53_RS18935 0.29 -2.1 182,589 + LRK53_RS18935 0.29 -1.5 182,589 + LRK53_RS18935 0.29 -2.8 182,589 + LRK53_RS18935 0.29 +0.1 182,589 + LRK53_RS18935 0.29 +0.6 182,589 + LRK53_RS18935 0.29 -0.0 182,589 + LRK53_RS18935 0.29 -1.4 182,590 - LRK53_RS18935 0.29 -1.5 182,590 - LRK53_RS18935 0.29 -1.4 182,590 - LRK53_RS18935 0.29 -2.1 182,590 - LRK53_RS18935 0.29 -0.1 182,590 - LRK53_RS18935 0.29 +0.5 182,590 - LRK53_RS18935 0.29 +0.1 182,590 - LRK53_RS18935 0.29 +0.4 182,590 - LRK53_RS18935 0.29 -1.3 182,590 - LRK53_RS18935 0.29 -0.5 182,590 - LRK53_RS18935 0.29 -1.3 182,590 - LRK53_RS18935 0.29 -0.8 182,664 + LRK53_RS18935 0.41 -0.4 182,664 + LRK53_RS18935 0.41 +0.2 182,664 + LRK53_RS18935 0.41 -1.1 182,664 + LRK53_RS18935 0.41 -0.3 182,665 - LRK53_RS18935 0.41 +0.2 182,665 - LRK53_RS18935 0.41 -1.9 182,665 - LRK53_RS18935 0.41 -1.3 182,665 - LRK53_RS18935 0.41 -0.5 182,665 - LRK53_RS18935 0.41 -0.9 182,665 - LRK53_RS18935 0.41 +0.1 182,665 - LRK53_RS18935 0.41 +0.1 182,665 - LRK53_RS18935 0.41 -0.4 182,665 - LRK53_RS18935 0.41 +2.1 182,709 + LRK53_RS18935 0.49 -1.1 182,798 + LRK53_RS18935 0.64 +0.5 182,799 - LRK53_RS18935 0.64 -1.1 182,799 - LRK53_RS18935 0.64 -1.8 182,799 - LRK53_RS18935 0.64 +0.8 182,829 + LRK53_RS18935 0.69 -0.8 182,829 + LRK53_RS18935 0.69 +0.1 182,829 + LRK53_RS18935 0.69 -1.1 182,829 + LRK53_RS18935 0.69 -0.3 182,829 + LRK53_RS18935 0.69 -1.1 182,829 + LRK53_RS18935 0.69 +1.1 182,829 + LRK53_RS18935 0.69 -0.3 182,829 + LRK53_RS18935 0.69 -1.5 182,829 + LRK53_RS18935 0.69 -0.9 182,830 - LRK53_RS18935 0.69 +0.1 182,830 - LRK53_RS18935 0.69 -0.8 182,830 - LRK53_RS18935 0.69 -0.6 182,830 - LRK53_RS18935 0.69 -1.4 182,830 - LRK53_RS18935 0.69 +0.9 182,830 - LRK53_RS18935 0.69 +0.1 182,830 - LRK53_RS18935 0.69 -1.1 182,883 + LRK53_RS18935 0.78 -0.8 182,956 - LRK53_RS18935 0.90 -0.5 183,133 + -1.1 183,134 - -1.4 183,134 - -0.5 183,134 - -1.8 183,134 - -0.8 183,310 + LRK53_RS18940 0.37 +0.1 183,310 + LRK53_RS18940 0.37 -3.0 183,310 + LRK53_RS18940 0.37 -0.7 183,310 + LRK53_RS18940 0.37 -1.9 183,310 + LRK53_RS18940 0.37 +0.6 183,311 - LRK53_RS18940 0.37 -0.3 183,311 - LRK53_RS18940 0.37 -0.1 183,311 - LRK53_RS18940 0.37 +0.2 183,325 + LRK53_RS18940 0.40 +0.6 183,397 + LRK53_RS18940 0.56 -1.6 183,397 + LRK53_RS18940 0.56 +2.4 183,397 + LRK53_RS18940 0.56 +1.7 183,397 + LRK53_RS18940 0.56 +0.5 183,397 + LRK53_RS18940 0.56 -2.7 183,397 + LRK53_RS18940 0.56 -1.3 183,397 + LRK53_RS18940 0.56 -2.0 183,397 + LRK53_RS18940 0.56 -0.9 183,397 + LRK53_RS18940 0.56 -1.2 183,397 + LRK53_RS18940 0.56 -0.5 183,398 - LRK53_RS18940 0.56 +0.4 183,398 - LRK53_RS18940 0.56 -1.5 183,398 - LRK53_RS18940 0.56 -1.4 183,398 - LRK53_RS18940 0.56 -2.4 183,398 - LRK53_RS18940 0.56 -2.3 183,398 - LRK53_RS18940 0.56 -3.1 183,398 - LRK53_RS18940 0.56 -0.0 183,398 - LRK53_RS18940 0.56 +0.5 183,398 - LRK53_RS18940 0.56 -1.0 183,398 - LRK53_RS18940 0.56 +1.5 183,398 - LRK53_RS18940 0.56 -0.9 183,398 - LRK53_RS18940 0.56 +0.1
Or see this region's nucleotide sequence