Experiment: LB_noSalt with Chloride 200 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt QEN71_RS42350 and QEN71_RS42355 overlap by 4 nucleotides QEN71_RS42355 and QEN71_RS42360 overlap by 4 nucleotides QEN71_RS42360 and QEN71_RS42365 are separated by 4 nucleotides QEN71_RS42365 and QEN71_RS42370 are separated by 64 nucleotides
QEN71_RS42350: QEN71_RS42350 - site-specific integrase, at 550,333 to 551,199
_RS42350
QEN71_RS42355: QEN71_RS42355 - tyrosine-type recombinase/integrase, at 551,196 to 552,188
_RS42355
QEN71_RS42360: QEN71_RS42360 - tyrosine-type recombinase/integrase, at 552,185 to 553,189
_RS42360
QEN71_RS42365: QEN71_RS42365 - IS21 family transposase, at 553,194 to 553,722
_RS42365
QEN71_RS42370: QEN71_RS42370 - sigma factor-like helix-turn-helix DNA-binding protein, at 553,787 to 554,290
_RS42370
Position (kb) 552
553
554 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 551.207 kb on + strand at 551.238 kb on - strand at 551.319 kb on + strand, within QEN71_RS42355 at 551.320 kb on - strand, within QEN71_RS42355 at 551.326 kb on + strand, within QEN71_RS42355 at 551.494 kb on + strand, within QEN71_RS42355 at 551.827 kb on - strand, within QEN71_RS42355 at 551.860 kb on + strand, within QEN71_RS42355 at 551.861 kb on - strand, within QEN71_RS42355 at 552.013 kb on - strand, within QEN71_RS42355 at 552.013 kb on - strand, within QEN71_RS42355 at 552.096 kb on + strand at 552.097 kb on - strand at 552.389 kb on + strand, within QEN71_RS42360 at 552.465 kb on - strand, within QEN71_RS42360 at 552.554 kb on + strand, within QEN71_RS42360 at 552.646 kb on - strand, within QEN71_RS42360 at 552.646 kb on - strand, within QEN71_RS42360 at 552.647 kb on + strand, within QEN71_RS42360 at 552.874 kb on + strand, within QEN71_RS42360 at 552.875 kb on - strand, within QEN71_RS42360 at 552.875 kb on - strand, within QEN71_RS42360 at 552.878 kb on + strand, within QEN71_RS42360 at 553.104 kb on + strand at 553.185 kb on + strand at 553.187 kb on + strand at 553.207 kb on + strand at 553.208 kb on - strand at 553.867 kb on - strand, within QEN71_RS42370 at 553.941 kb on + strand, within QEN71_RS42370 at 553.992 kb on + strand, within QEN71_RS42370 at 553.992 kb on + strand, within QEN71_RS42370 at 553.992 kb on + strand, within QEN71_RS42370 at 553.993 kb on - strand, within QEN71_RS42370 at 553.993 kb on - strand, within QEN71_RS42370 at 554.045 kb on + strand, within QEN71_RS42370 at 554.046 kb on - strand, within QEN71_RS42370 at 554.046 kb on - strand, within QEN71_RS42370 at 554.187 kb on + strand, within QEN71_RS42370 at 554.187 kb on + strand, within QEN71_RS42370 at 554.187 kb on + strand, within QEN71_RS42370 at 554.188 kb on - strand, within QEN71_RS42370
Per-strain Table
Position Strand Gene LocusTag Fraction LB_noSalt with Chloride 200 mM remove 551,207 + -0.9 551,238 - +0.0 551,319 + QEN71_RS42355 0.12 -0.5 551,320 - QEN71_RS42355 0.12 +0.5 551,326 + QEN71_RS42355 0.13 -1.6 551,494 + QEN71_RS42355 0.30 -0.9 551,827 - QEN71_RS42355 0.64 +0.1 551,860 + QEN71_RS42355 0.67 -1.3 551,861 - QEN71_RS42355 0.67 +0.1 552,013 - QEN71_RS42355 0.82 -0.3 552,013 - QEN71_RS42355 0.82 -0.0 552,096 + +0.2 552,097 - -0.1 552,389 + QEN71_RS42360 0.20 +0.3 552,465 - QEN71_RS42360 0.28 +1.5 552,554 + QEN71_RS42360 0.37 -0.6 552,646 - QEN71_RS42360 0.46 +0.1 552,646 - QEN71_RS42360 0.46 -1.3 552,647 + QEN71_RS42360 0.46 -0.3 552,874 + QEN71_RS42360 0.69 +0.3 552,875 - QEN71_RS42360 0.69 -0.4 552,875 - QEN71_RS42360 0.69 +1.0 552,878 + QEN71_RS42360 0.69 -0.7 553,104 + -0.7 553,185 + -0.3 553,187 + +2.6 553,207 + -0.2 553,208 - -1.0 553,867 - QEN71_RS42370 0.16 -1.0 553,941 + QEN71_RS42370 0.31 +2.0 553,992 + QEN71_RS42370 0.41 +0.9 553,992 + QEN71_RS42370 0.41 +0.1 553,992 + QEN71_RS42370 0.41 -1.2 553,993 - QEN71_RS42370 0.41 -0.3 553,993 - QEN71_RS42370 0.41 +1.3 554,045 + QEN71_RS42370 0.51 +0.5 554,046 - QEN71_RS42370 0.51 +0.1 554,046 - QEN71_RS42370 0.51 -2.9 554,187 + QEN71_RS42370 0.79 +0.2 554,187 + QEN71_RS42370 0.79 -1.0 554,187 + QEN71_RS42370 0.79 -2.5 554,188 - QEN71_RS42370 0.80 +0.1
Or see this region's nucleotide sequence