Experiment: LB_noSalt with Chloride 200 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt QEN71_RS42140 and QEN71_RS42145 are separated by 12 nucleotides QEN71_RS42145 and QEN71_RS42150 overlap by 8 nucleotides QEN71_RS42150 and QEN71_RS42155 are separated by 52 nucleotides QEN71_RS42155 and QEN71_RS42160 are separated by 7 nucleotides QEN71_RS42160 and QEN71_RS42165 are separated by 11 nucleotides
QEN71_RS42140: QEN71_RS42140 - ATP-grasp domain-containing protein, at 512,301 to 513,632
_RS42140
QEN71_RS42145: QEN71_RS42145 - hypothetical protein, at 513,645 to 513,827
_RS42145
QEN71_RS42150: QEN71_RS42150 - hypothetical protein, at 513,820 to 514,074
_RS42150
QEN71_RS42155: QEN71_RS42155 - hypothetical protein, at 514,127 to 514,372
_RS42155
QEN71_RS42160: QEN71_RS42160 - hypothetical protein, at 514,380 to 514,688
_RS42160
QEN71_RS42165: QEN71_RS42165 - hypothetical protein, at 514,700 to 515,398
_RS42165
Position (kb) 513
514
515 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 512.922 kb on + strand, within QEN71_RS42140 at 512.970 kb on - strand, within QEN71_RS42140 at 512.970 kb on - strand, within QEN71_RS42140 at 512.970 kb on - strand, within QEN71_RS42140 at 512.972 kb on - strand, within QEN71_RS42140 at 512.972 kb on - strand, within QEN71_RS42140 at 513.075 kb on + strand, within QEN71_RS42140 at 513.123 kb on + strand, within QEN71_RS42140 at 513.124 kb on - strand, within QEN71_RS42140 at 513.129 kb on + strand, within QEN71_RS42140 at 513.130 kb on - strand, within QEN71_RS42140 at 513.130 kb on - strand, within QEN71_RS42140 at 513.179 kb on + strand, within QEN71_RS42140 at 513.180 kb on - strand, within QEN71_RS42140 at 513.397 kb on - strand, within QEN71_RS42140 at 513.457 kb on - strand, within QEN71_RS42140 at 513.457 kb on - strand, within QEN71_RS42140 at 513.520 kb on + strand at 513.520 kb on + strand at 513.520 kb on + strand at 513.520 kb on + strand at 513.520 kb on + strand at 513.521 kb on - strand at 513.551 kb on - strand at 513.551 kb on - strand at 513.551 kb on - strand at 513.651 kb on - strand at 513.651 kb on - strand at 513.674 kb on + strand, within QEN71_RS42145 at 513.821 kb on + strand at 513.875 kb on + strand, within QEN71_RS42150 at 513.875 kb on + strand, within QEN71_RS42150 at 513.875 kb on + strand, within QEN71_RS42150 at 513.875 kb on + strand, within QEN71_RS42150 at 513.875 kb on + strand, within QEN71_RS42150 at 513.903 kb on - strand, within QEN71_RS42150 at 513.903 kb on - strand, within QEN71_RS42150 at 513.903 kb on - strand, within QEN71_RS42150 at 513.925 kb on + strand, within QEN71_RS42150 at 513.925 kb on + strand, within QEN71_RS42150 at 513.926 kb on - strand, within QEN71_RS42150 at 513.974 kb on + strand, within QEN71_RS42150 at 514.083 kb on + strand at 514.083 kb on + strand at 514.084 kb on - strand at 514.097 kb on - strand at 514.097 kb on - strand at 514.165 kb on - strand, within QEN71_RS42155 at 514.165 kb on - strand, within QEN71_RS42155 at 514.194 kb on - strand, within QEN71_RS42155 at 514.227 kb on + strand, within QEN71_RS42155 at 514.228 kb on - strand, within QEN71_RS42155 at 514.240 kb on + strand, within QEN71_RS42155 at 514.240 kb on + strand, within QEN71_RS42155 at 514.240 kb on + strand, within QEN71_RS42155 at 514.240 kb on + strand, within QEN71_RS42155 at 514.240 kb on + strand, within QEN71_RS42155 at 514.240 kb on + strand, within QEN71_RS42155 at 514.242 kb on + strand, within QEN71_RS42155 at 514.304 kb on + strand, within QEN71_RS42155 at 514.304 kb on + strand, within QEN71_RS42155 at 514.305 kb on - strand, within QEN71_RS42155 at 514.305 kb on - strand, within QEN71_RS42155 at 514.455 kb on - strand, within QEN71_RS42160 at 514.456 kb on + strand, within QEN71_RS42160 at 514.457 kb on - strand, within QEN71_RS42160 at 514.640 kb on + strand, within QEN71_RS42160 at 514.640 kb on + strand, within QEN71_RS42160 at 514.671 kb on + strand at 514.742 kb on + strand at 514.743 kb on - strand at 514.789 kb on - strand, within QEN71_RS42165 at 514.908 kb on + strand, within QEN71_RS42165 at 514.909 kb on - strand, within QEN71_RS42165 at 514.909 kb on - strand, within QEN71_RS42165 at 514.956 kb on + strand, within QEN71_RS42165 at 514.968 kb on + strand, within QEN71_RS42165
Per-strain Table
Position Strand Gene LocusTag Fraction LB_noSalt with Chloride 200 mM remove 512,922 + QEN71_RS42140 0.47 +1.0 512,970 - QEN71_RS42140 0.50 +1.3 512,970 - QEN71_RS42140 0.50 +0.3 512,970 - QEN71_RS42140 0.50 -2.1 512,972 - QEN71_RS42140 0.50 -0.5 512,972 - QEN71_RS42140 0.50 -1.2 513,075 + QEN71_RS42140 0.58 -0.9 513,123 + QEN71_RS42140 0.62 -0.8 513,124 - QEN71_RS42140 0.62 -3.0 513,129 + QEN71_RS42140 0.62 -1.6 513,130 - QEN71_RS42140 0.62 -0.3 513,130 - QEN71_RS42140 0.62 -2.3 513,179 + QEN71_RS42140 0.66 -0.5 513,180 - QEN71_RS42140 0.66 -0.8 513,397 - QEN71_RS42140 0.82 -2.3 513,457 - QEN71_RS42140 0.87 -1.1 513,457 - QEN71_RS42140 0.87 -1.3 513,520 + -0.6 513,520 + +0.2 513,520 + -2.4 513,520 + +0.0 513,520 + -0.1 513,521 - +1.3 513,551 - -1.1 513,551 - -1.2 513,551 - -1.1 513,651 - -2.8 513,651 - -1.1 513,674 + QEN71_RS42145 0.16 -0.1 513,821 + -0.8 513,875 + QEN71_RS42150 0.22 -1.6 513,875 + QEN71_RS42150 0.22 -2.0 513,875 + QEN71_RS42150 0.22 +1.0 513,875 + QEN71_RS42150 0.22 -0.4 513,875 + QEN71_RS42150 0.22 -0.3 513,903 - QEN71_RS42150 0.33 -2.4 513,903 - QEN71_RS42150 0.33 -0.6 513,903 - QEN71_RS42150 0.33 -1.0 513,925 + QEN71_RS42150 0.41 -1.0 513,925 + QEN71_RS42150 0.41 -1.2 513,926 - QEN71_RS42150 0.42 -1.5 513,974 + QEN71_RS42150 0.60 -0.0 514,083 + +1.0 514,083 + -0.6 514,084 - -2.0 514,097 - -0.2 514,097 - -0.5 514,165 - QEN71_RS42155 0.15 +1.2 514,165 - QEN71_RS42155 0.15 +0.5 514,194 - QEN71_RS42155 0.27 -1.7 514,227 + QEN71_RS42155 0.41 -1.8 514,228 - QEN71_RS42155 0.41 -2.3 514,240 + QEN71_RS42155 0.46 -2.3 514,240 + QEN71_RS42155 0.46 +0.0 514,240 + QEN71_RS42155 0.46 -0.8 514,240 + QEN71_RS42155 0.46 +0.6 514,240 + QEN71_RS42155 0.46 -1.0 514,240 + QEN71_RS42155 0.46 -1.2 514,242 + QEN71_RS42155 0.47 -0.3 514,304 + QEN71_RS42155 0.72 -1.5 514,304 + QEN71_RS42155 0.72 -0.3 514,305 - QEN71_RS42155 0.72 -1.7 514,305 - QEN71_RS42155 0.72 -0.3 514,455 - QEN71_RS42160 0.24 +0.7 514,456 + QEN71_RS42160 0.25 -0.8 514,457 - QEN71_RS42160 0.25 -0.6 514,640 + QEN71_RS42160 0.84 +0.6 514,640 + QEN71_RS42160 0.84 -0.9 514,671 + -0.1 514,742 + -0.0 514,743 - +1.0 514,789 - QEN71_RS42165 0.13 +1.4 514,908 + QEN71_RS42165 0.30 -0.3 514,909 - QEN71_RS42165 0.30 -1.2 514,909 - QEN71_RS42165 0.30 -0.8 514,956 + QEN71_RS42165 0.37 -2.4 514,968 + QEN71_RS42165 0.38 -0.2
Or see this region's nucleotide sequence