Experiment: LB_noSalt with Chloride 200 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt QEN71_RS36850 and QEN71_RS36855 are separated by 2 nucleotides QEN71_RS36855 and QEN71_RS36860 are separated by 82 nucleotides QEN71_RS36860 and QEN71_RS36865 are separated by 85 nucleotides QEN71_RS36865 and QEN71_RS36870 are separated by 59 nucleotides
QEN71_RS36850: QEN71_RS36850 - type III secretion protein, at 1,644,850 to 1,646,028
_RS36850
QEN71_RS36855: QEN71_RS36855 - type III secretion protein, at 1,646,031 to 1,646,273
_RS36855
QEN71_RS36860: QEN71_RS36860 - hypothetical protein, at 1,646,356 to 1,646,577
_RS36860
QEN71_RS36865: QEN71_RS36865 - CesT family type III secretion system chaperone, at 1,646,663 to 1,647,118
_RS36865
QEN71_RS36870: QEN71_RS36870 - MEKHLA domain-containing protein, at 1,647,178 to 1,647,636
_RS36870
Position (kb)
1646
1647 Strain fitness (log2 ratio)
-2
-1
0
1 at 1645.120 kb on - strand, within QEN71_RS36850 at 1645.686 kb on + strand, within QEN71_RS36850 at 1645.756 kb on - strand, within QEN71_RS36850 at 1645.804 kb on + strand, within QEN71_RS36850 at 1645.837 kb on + strand, within QEN71_RS36850 at 1646.019 kb on - strand at 1646.034 kb on + strand at 1646.034 kb on + strand at 1646.035 kb on - strand at 1646.208 kb on + strand, within QEN71_RS36855 at 1646.209 kb on - strand, within QEN71_RS36855 at 1646.305 kb on + strand at 1646.575 kb on + strand at 1646.646 kb on + strand at 1647.146 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_noSalt with Chloride 200 mM remove 1,645,120 - QEN71_RS36850 0.23 +0.7 1,645,686 + QEN71_RS36850 0.71 +0.9 1,645,756 - QEN71_RS36850 0.77 -0.7 1,645,804 + QEN71_RS36850 0.81 -0.6 1,645,837 + QEN71_RS36850 0.84 -2.0 1,646,019 - +0.1 1,646,034 + +0.8 1,646,034 + -0.6 1,646,035 - -1.4 1,646,208 + QEN71_RS36855 0.73 -1.5 1,646,209 - QEN71_RS36855 0.73 -0.2 1,646,305 + -0.7 1,646,575 + +0.1 1,646,646 + -0.5 1,647,146 - -1.1
Or see this region's nucleotide sequence