Experiment: LB_noSalt with Chloride 200 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt QEN71_RS36830 and QEN71_RS36835 overlap by 23 nucleotides QEN71_RS36835 and QEN71_RS36840 are separated by 29 nucleotides QEN71_RS36840 and QEN71_RS36845 overlap by 4 nucleotides
QEN71_RS36830: QEN71_RS36830 - type III secretion system cytoplasmic ring protein SctQ, at 1,641,676 to 1,642,884
_RS36830
QEN71_RS36835: QEN71_RS36835 - type III secretion system export apparatus subunit SctR, at 1,642,862 to 1,643,518
_RS36835
QEN71_RS36840: QEN71_RS36840 - type III secretion system export apparatus subunit SctS, at 1,643,548 to 1,643,811
_RS36840
QEN71_RS36845: QEN71_RS36845 - hypothetical protein, at 1,643,808 to 1,644,743
_RS36845
Position (kb)
1642
1643
1644 Strain fitness (log2 ratio)
-1
0
1 at 1642.167 kb on + strand, within QEN71_RS36830 at 1642.268 kb on + strand, within QEN71_RS36830 at 1642.452 kb on - strand, within QEN71_RS36830 at 1642.466 kb on + strand, within QEN71_RS36830 at 1642.509 kb on - strand, within QEN71_RS36830 at 1642.842 kb on - strand at 1642.861 kb on + strand at 1642.955 kb on + strand, within QEN71_RS36835 at 1642.955 kb on + strand, within QEN71_RS36835 at 1643.060 kb on + strand, within QEN71_RS36835 at 1643.602 kb on + strand, within QEN71_RS36840 at 1643.602 kb on + strand, within QEN71_RS36840
Per-strain Table
Position Strand Gene LocusTag Fraction LB_noSalt with Chloride 200 mM remove 1,642,167 + QEN71_RS36830 0.41 +0.3 1,642,268 + QEN71_RS36830 0.49 +0.1 1,642,452 - QEN71_RS36830 0.64 +1.1 1,642,466 + QEN71_RS36830 0.65 +0.0 1,642,509 - QEN71_RS36830 0.69 -0.0 1,642,842 - +0.5 1,642,861 + -0.6 1,642,955 + QEN71_RS36835 0.14 -1.4 1,642,955 + QEN71_RS36835 0.14 -0.7 1,643,060 + QEN71_RS36835 0.30 +0.0 1,643,602 + QEN71_RS36840 0.20 -0.7 1,643,602 + QEN71_RS36840 0.20 -1.0
Or see this region's nucleotide sequence