Experiment: LB_noSalt with Chloride 200 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt QEN71_RS32910 and QEN71_RS32915 are separated by 41 nucleotides QEN71_RS32915 and QEN71_RS32920 are separated by 32 nucleotides QEN71_RS32920 and QEN71_RS32925 are separated by 74 nucleotides
QEN71_RS32910: QEN71_RS32910 - DUF2182 domain-containing protein, at 714,111 to 714,920
_RS32910
QEN71_RS32915: QEN71_RS32915 - SDR family oxidoreductase, at 714,962 to 715,732
_RS32915
QEN71_RS32920: QEN71_RS32920 - zinc-binding alcohol dehydrogenase family protein, at 715,765 to 716,736
_RS32920
QEN71_RS32925: QEN71_RS32925 - alpha/beta hydrolase, at 716,811 to 717,665
_RS32925
Position (kb)
715
716
717 Strain fitness (log2 ratio)
-2
-1
0
1 at 715.158 kb on + strand, within QEN71_RS32915 at 715.159 kb on - strand, within QEN71_RS32915 at 715.159 kb on - strand, within QEN71_RS32915 at 715.159 kb on - strand, within QEN71_RS32915 at 715.290 kb on + strand, within QEN71_RS32915 at 715.719 kb on - strand at 715.967 kb on + strand, within QEN71_RS32920 at 715.968 kb on - strand, within QEN71_RS32920 at 715.977 kb on + strand, within QEN71_RS32920 at 715.978 kb on - strand, within QEN71_RS32920 at 716.686 kb on - strand at 716.687 kb on + strand at 716.688 kb on - strand at 716.748 kb on + strand at 716.754 kb on - strand at 716.754 kb on - strand at 716.754 kb on - strand at 716.992 kb on + strand, within QEN71_RS32925 at 717.004 kb on + strand, within QEN71_RS32925 at 717.005 kb on - strand, within QEN71_RS32925 at 717.005 kb on - strand, within QEN71_RS32925 at 717.023 kb on - strand, within QEN71_RS32925 at 717.066 kb on + strand, within QEN71_RS32925 at 717.066 kb on + strand, within QEN71_RS32925 at 717.067 kb on - strand, within QEN71_RS32925 at 717.094 kb on + strand, within QEN71_RS32925 at 717.094 kb on + strand, within QEN71_RS32925 at 717.095 kb on - strand, within QEN71_RS32925 at 717.095 kb on - strand, within QEN71_RS32925 at 717.095 kb on - strand, within QEN71_RS32925 at 717.157 kb on + strand, within QEN71_RS32925 at 717.157 kb on + strand, within QEN71_RS32925 at 717.158 kb on - strand, within QEN71_RS32925 at 717.232 kb on + strand, within QEN71_RS32925 at 717.274 kb on + strand, within QEN71_RS32925 at 717.275 kb on - strand, within QEN71_RS32925 at 717.367 kb on + strand, within QEN71_RS32925 at 717.367 kb on + strand, within QEN71_RS32925 at 717.368 kb on - strand, within QEN71_RS32925 at 717.437 kb on + strand, within QEN71_RS32925 at 717.437 kb on + strand, within QEN71_RS32925 at 717.437 kb on + strand, within QEN71_RS32925 at 717.437 kb on + strand, within QEN71_RS32925 at 717.437 kb on + strand, within QEN71_RS32925 at 717.437 kb on + strand, within QEN71_RS32925 at 717.437 kb on + strand, within QEN71_RS32925 at 717.437 kb on + strand, within QEN71_RS32925 at 717.438 kb on - strand, within QEN71_RS32925 at 717.438 kb on - strand, within QEN71_RS32925 at 717.439 kb on + strand, within QEN71_RS32925 at 717.545 kb on + strand, within QEN71_RS32925 at 717.545 kb on + strand, within QEN71_RS32925 at 717.545 kb on + strand, within QEN71_RS32925 at 717.545 kb on + strand, within QEN71_RS32925 at 717.546 kb on - strand, within QEN71_RS32925 at 717.613 kb on + strand at 717.613 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_noSalt with Chloride 200 mM remove 715,158 + QEN71_RS32915 0.25 +0.2 715,159 - QEN71_RS32915 0.26 -0.2 715,159 - QEN71_RS32915 0.26 +0.1 715,159 - QEN71_RS32915 0.26 +0.4 715,290 + QEN71_RS32915 0.43 -1.3 715,719 - -0.5 715,967 + QEN71_RS32920 0.21 -2.5 715,968 - QEN71_RS32920 0.21 -0.5 715,977 + QEN71_RS32920 0.22 -1.8 715,978 - QEN71_RS32920 0.22 -0.1 716,686 - +1.0 716,687 + +0.6 716,688 - +0.2 716,748 + -0.3 716,754 - -0.8 716,754 - -1.0 716,754 - -1.0 716,992 + QEN71_RS32925 0.21 +0.1 717,004 + QEN71_RS32925 0.23 -1.1 717,005 - QEN71_RS32925 0.23 -1.1 717,005 - QEN71_RS32925 0.23 -1.0 717,023 - QEN71_RS32925 0.25 +0.0 717,066 + QEN71_RS32925 0.30 -0.3 717,066 + QEN71_RS32925 0.30 +1.1 717,067 - QEN71_RS32925 0.30 +0.2 717,094 + QEN71_RS32925 0.33 +0.6 717,094 + QEN71_RS32925 0.33 -0.5 717,095 - QEN71_RS32925 0.33 -0.2 717,095 - QEN71_RS32925 0.33 -0.6 717,095 - QEN71_RS32925 0.33 +0.6 717,157 + QEN71_RS32925 0.40 -0.9 717,157 + QEN71_RS32925 0.40 -1.9 717,158 - QEN71_RS32925 0.41 +0.3 717,232 + QEN71_RS32925 0.49 +0.1 717,274 + QEN71_RS32925 0.54 +0.1 717,275 - QEN71_RS32925 0.54 +0.1 717,367 + QEN71_RS32925 0.65 -0.2 717,367 + QEN71_RS32925 0.65 -0.6 717,368 - QEN71_RS32925 0.65 -0.3 717,437 + QEN71_RS32925 0.73 -1.1 717,437 + QEN71_RS32925 0.73 +0.3 717,437 + QEN71_RS32925 0.73 +0.6 717,437 + QEN71_RS32925 0.73 -2.0 717,437 + QEN71_RS32925 0.73 -0.8 717,437 + QEN71_RS32925 0.73 -0.4 717,437 + QEN71_RS32925 0.73 +0.3 717,437 + QEN71_RS32925 0.73 -0.6 717,438 - QEN71_RS32925 0.73 +1.3 717,438 - QEN71_RS32925 0.73 +0.4 717,439 + QEN71_RS32925 0.73 -0.4 717,545 + QEN71_RS32925 0.86 +0.7 717,545 + QEN71_RS32925 0.86 +1.4 717,545 + QEN71_RS32925 0.86 +0.5 717,545 + QEN71_RS32925 0.86 -0.2 717,546 - QEN71_RS32925 0.86 +0.4 717,613 + -0.9 717,613 + +0.4
Or see this region's nucleotide sequence