Experiment: LB_noSalt with Chloride 200 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt QEN71_RS32475 and QEN71_RS32480 overlap by 4 nucleotides QEN71_RS32480 and QEN71_RS32485 are separated by 191 nucleotides QEN71_RS32485 and QEN71_RS32490 are separated by 366 nucleotides
QEN71_RS32475: QEN71_RS32475 - fumarate reductase/succinate dehydrogenase flavoprotein subunit, at 622,301 to 624,067
_RS32475
QEN71_RS32480: QEN71_RS32480 - GntR family transcriptional regulator, at 624,064 to 624,855
_RS32480
QEN71_RS32485: QEN71_RS32485 - aminotransferase class V-fold PLP-dependent enzyme, at 625,047 to 626,390
_RS32485
QEN71_RS32490: QEN71_RS32490 - 5-dehydro-4-deoxyglucarate dehydratase, at 626,757 to 627,674
_RS32490
Position (kb) 625
626
627 Strain fitness (log2 ratio) -2
-1
0
1
2 at 624.053 kb on + strand at 624.054 kb on - strand at 624.103 kb on - strand at 624.103 kb on - strand at 624.159 kb on - strand, within QEN71_RS32480 at 624.159 kb on - strand, within QEN71_RS32480 at 624.170 kb on + strand, within QEN71_RS32480 at 624.722 kb on - strand, within QEN71_RS32480 at 624.759 kb on - strand, within QEN71_RS32480 at 624.759 kb on - strand, within QEN71_RS32480 at 624.920 kb on + strand at 624.934 kb on - strand at 625.519 kb on + strand, within QEN71_RS32485 at 626.173 kb on + strand, within QEN71_RS32485 at 626.284 kb on - strand at 626.337 kb on + strand at 626.337 kb on + strand at 626.337 kb on + strand at 626.341 kb on + strand at 626.342 kb on - strand at 626.342 kb on - strand at 626.392 kb on - strand at 626.395 kb on + strand at 626.395 kb on + strand at 626.395 kb on + strand at 626.396 kb on - strand at 626.419 kb on - strand at 626.454 kb on - strand at 626.497 kb on + strand at 626.632 kb on - strand at 626.961 kb on - strand, within QEN71_RS32490 at 626.961 kb on - strand, within QEN71_RS32490 at 627.020 kb on + strand, within QEN71_RS32490 at 627.059 kb on + strand, within QEN71_RS32490 at 627.060 kb on - strand, within QEN71_RS32490 at 627.060 kb on - strand, within QEN71_RS32490 at 627.061 kb on + strand, within QEN71_RS32490 at 627.061 kb on + strand, within QEN71_RS32490 at 627.062 kb on - strand, within QEN71_RS32490 at 627.062 kb on - strand, within QEN71_RS32490 at 627.195 kb on - strand, within QEN71_RS32490 at 627.195 kb on - strand, within QEN71_RS32490 at 627.248 kb on - strand, within QEN71_RS32490 at 627.248 kb on - strand, within QEN71_RS32490 at 627.306 kb on - strand, within QEN71_RS32490 at 627.306 kb on - strand, within QEN71_RS32490 at 627.331 kb on + strand, within QEN71_RS32490 at 627.332 kb on - strand, within QEN71_RS32490
Per-strain Table
Position Strand Gene LocusTag Fraction LB_noSalt with Chloride 200 mM remove 624,053 + +0.2 624,054 - -0.4 624,103 - -0.6 624,103 - +0.7 624,159 - QEN71_RS32480 0.12 +0.6 624,159 - QEN71_RS32480 0.12 -0.0 624,170 + QEN71_RS32480 0.13 -0.5 624,722 - QEN71_RS32480 0.83 -2.6 624,759 - QEN71_RS32480 0.88 -1.4 624,759 - QEN71_RS32480 0.88 -0.2 624,920 + -0.6 624,934 - -0.2 625,519 + QEN71_RS32485 0.35 -1.6 626,173 + QEN71_RS32485 0.84 -0.2 626,284 - +0.2 626,337 + +0.2 626,337 + -2.5 626,337 + +0.3 626,341 + -0.0 626,342 - -0.4 626,342 - +1.8 626,392 - -1.3 626,395 + +0.0 626,395 + +0.4 626,395 + -0.9 626,396 - -0.5 626,419 - +0.1 626,454 - +0.1 626,497 + -0.3 626,632 - -1.5 626,961 - QEN71_RS32490 0.22 +1.8 626,961 - QEN71_RS32490 0.22 -0.6 627,020 + QEN71_RS32490 0.29 +0.1 627,059 + QEN71_RS32490 0.33 -0.0 627,060 - QEN71_RS32490 0.33 +0.0 627,060 - QEN71_RS32490 0.33 +1.3 627,061 + QEN71_RS32490 0.33 +0.6 627,061 + QEN71_RS32490 0.33 +0.2 627,062 - QEN71_RS32490 0.33 -0.7 627,062 - QEN71_RS32490 0.33 -0.9 627,195 - QEN71_RS32490 0.48 +0.1 627,195 - QEN71_RS32490 0.48 -0.5 627,248 - QEN71_RS32490 0.53 -0.4 627,248 - QEN71_RS32490 0.53 -0.5 627,306 - QEN71_RS32490 0.60 -1.1 627,306 - QEN71_RS32490 0.60 +0.1 627,331 + QEN71_RS32490 0.63 -0.1 627,332 - QEN71_RS32490 0.63 -0.8
Or see this region's nucleotide sequence