Experiment: LB_noSalt with Chloride 200 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt QEN71_RS27780 and QEN71_RS27785 overlap by 1 nucleotides QEN71_RS27785 and QEN71_RS27790 are separated by 219 nucleotides QEN71_RS27790 and QEN71_RS27795 are separated by 19 nucleotides
QEN71_RS27780: QEN71_RS27780 - cytochrome bc complex cytochrome b subunit, at 6,186,370 to 6,187,752
_RS27780
QEN71_RS27785: QEN71_RS27785 - ubiquinol-cytochrome c reductase iron-sulfur subunit, at 6,187,752 to 6,188,369
_RS27785
QEN71_RS27790: QEN71_RS27790 - Nif3-like dinuclear metal center hexameric protein, at 6,188,589 to 6,189,335
_RS27790
QEN71_RS27795: QEN71_RS27795 - Do family serine endopeptidase, at 6,189,355 to 6,190,563
_RS27795
Position (kb)
6187
6188
6189 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 6187.010 kb on + strand, within QEN71_RS27780 at 6187.623 kb on - strand at 6187.730 kb on - strand at 6187.730 kb on - strand at 6187.730 kb on - strand at 6187.754 kb on - strand at 6187.870 kb on + strand, within QEN71_RS27785 at 6187.871 kb on - strand, within QEN71_RS27785 at 6187.871 kb on - strand, within QEN71_RS27785 at 6188.205 kb on - strand, within QEN71_RS27785 at 6188.206 kb on - strand, within QEN71_RS27785 at 6188.262 kb on - strand, within QEN71_RS27785 at 6188.262 kb on - strand, within QEN71_RS27785 at 6188.423 kb on + strand at 6188.423 kb on + strand at 6188.424 kb on - strand at 6188.424 kb on - strand at 6188.424 kb on - strand at 6188.450 kb on - strand at 6188.450 kb on - strand at 6188.450 kb on - strand at 6188.488 kb on + strand at 6188.488 kb on + strand at 6188.488 kb on + strand at 6188.488 kb on + strand at 6188.489 kb on - strand at 6188.534 kb on + strand at 6188.534 kb on + strand at 6188.534 kb on + strand at 6188.534 kb on + strand at 6188.535 kb on - strand at 6188.535 kb on - strand at 6188.537 kb on + strand at 6188.574 kb on + strand at 6188.600 kb on + strand at 6188.600 kb on + strand at 6188.600 kb on + strand at 6188.601 kb on - strand at 6188.601 kb on - strand at 6188.601 kb on - strand at 6188.606 kb on + strand at 6188.606 kb on + strand at 6188.607 kb on - strand at 6188.607 kb on - strand at 6188.607 kb on - strand at 6188.607 kb on - strand at 6188.607 kb on - strand at 6188.608 kb on + strand at 6188.610 kb on + strand at 6188.610 kb on + strand at 6188.610 kb on + strand at 6188.610 kb on + strand at 6188.610 kb on + strand at 6188.610 kb on + strand at 6188.610 kb on + strand at 6188.610 kb on + strand at 6188.610 kb on + strand at 6188.610 kb on + strand at 6188.610 kb on + strand at 6188.610 kb on + strand at 6188.610 kb on + strand at 6188.610 kb on + strand at 6188.610 kb on + strand at 6188.610 kb on + strand at 6188.610 kb on + strand at 6188.610 kb on + strand at 6188.610 kb on + strand at 6188.611 kb on - strand at 6188.611 kb on - strand at 6188.611 kb on - strand at 6188.611 kb on - strand at 6188.611 kb on - strand at 6188.611 kb on - strand at 6188.611 kb on - strand at 6188.611 kb on - strand at 6188.611 kb on - strand at 6188.611 kb on - strand at 6188.611 kb on - strand at 6188.611 kb on - strand at 6188.611 kb on - strand at 6188.611 kb on - strand at 6188.611 kb on - strand at 6188.611 kb on - strand at 6188.611 kb on - strand at 6188.611 kb on - strand at 6188.611 kb on - strand at 6188.612 kb on + strand at 6188.612 kb on + strand at 6188.612 kb on + strand at 6188.613 kb on - strand at 6188.613 kb on - strand at 6188.613 kb on - strand at 6188.613 kb on - strand at 6188.651 kb on + strand at 6188.651 kb on + strand at 6188.729 kb on + strand, within QEN71_RS27790 at 6188.729 kb on + strand, within QEN71_RS27790 at 6188.729 kb on + strand, within QEN71_RS27790 at 6188.729 kb on + strand, within QEN71_RS27790 at 6188.729 kb on + strand, within QEN71_RS27790 at 6188.729 kb on + strand, within QEN71_RS27790 at 6188.729 kb on + strand, within QEN71_RS27790 at 6188.729 kb on + strand, within QEN71_RS27790 at 6188.729 kb on + strand, within QEN71_RS27790 at 6188.729 kb on + strand, within QEN71_RS27790 at 6188.729 kb on + strand, within QEN71_RS27790 at 6188.729 kb on + strand, within QEN71_RS27790 at 6188.729 kb on + strand, within QEN71_RS27790 at 6188.729 kb on + strand, within QEN71_RS27790 at 6188.729 kb on + strand, within QEN71_RS27790 at 6188.729 kb on + strand, within QEN71_RS27790 at 6188.729 kb on + strand, within QEN71_RS27790 at 6188.730 kb on - strand, within QEN71_RS27790 at 6188.730 kb on - strand, within QEN71_RS27790 at 6188.730 kb on - strand, within QEN71_RS27790 at 6188.730 kb on - strand, within QEN71_RS27790 at 6188.730 kb on - strand, within QEN71_RS27790 at 6188.730 kb on - strand, within QEN71_RS27790 at 6188.730 kb on - strand, within QEN71_RS27790 at 6188.730 kb on - strand, within QEN71_RS27790 at 6188.730 kb on - strand, within QEN71_RS27790 at 6188.730 kb on - strand, within QEN71_RS27790 at 6188.730 kb on - strand, within QEN71_RS27790 at 6188.730 kb on - strand, within QEN71_RS27790 at 6188.730 kb on - strand, within QEN71_RS27790 at 6188.730 kb on - strand, within QEN71_RS27790 at 6188.730 kb on - strand, within QEN71_RS27790 at 6188.730 kb on - strand, within QEN71_RS27790 at 6188.730 kb on - strand, within QEN71_RS27790 at 6188.730 kb on - strand, within QEN71_RS27790 at 6188.730 kb on - strand, within QEN71_RS27790 at 6188.730 kb on - strand, within QEN71_RS27790 at 6188.730 kb on - strand, within QEN71_RS27790 at 6188.730 kb on - strand, within QEN71_RS27790 at 6188.730 kb on - strand, within QEN71_RS27790 at 6188.730 kb on - strand, within QEN71_RS27790 at 6188.730 kb on - strand, within QEN71_RS27790 at 6188.730 kb on - strand, within QEN71_RS27790 at 6188.730 kb on - strand, within QEN71_RS27790 at 6188.764 kb on + strand, within QEN71_RS27790 at 6188.765 kb on - strand, within QEN71_RS27790 at 6188.765 kb on - strand, within QEN71_RS27790 at 6188.765 kb on - strand, within QEN71_RS27790 at 6188.765 kb on - strand, within QEN71_RS27790 at 6188.765 kb on - strand, within QEN71_RS27790 at 6188.800 kb on + strand, within QEN71_RS27790 at 6188.801 kb on - strand, within QEN71_RS27790 at 6188.801 kb on - strand, within QEN71_RS27790 at 6188.801 kb on - strand, within QEN71_RS27790 at 6188.862 kb on + strand, within QEN71_RS27790 at 6188.863 kb on - strand, within QEN71_RS27790 at 6188.863 kb on - strand, within QEN71_RS27790 at 6188.863 kb on - strand, within QEN71_RS27790 at 6189.020 kb on - strand, within QEN71_RS27790 at 6189.020 kb on - strand, within QEN71_RS27790 at 6189.020 kb on - strand, within QEN71_RS27790 at 6189.020 kb on - strand, within QEN71_RS27790 at 6189.020 kb on - strand, within QEN71_RS27790 at 6189.097 kb on + strand, within QEN71_RS27790 at 6189.097 kb on + strand, within QEN71_RS27790 at 6189.097 kb on + strand, within QEN71_RS27790 at 6189.097 kb on + strand, within QEN71_RS27790 at 6189.097 kb on + strand, within QEN71_RS27790 at 6189.098 kb on - strand, within QEN71_RS27790 at 6189.098 kb on - strand, within QEN71_RS27790 at 6189.110 kb on + strand, within QEN71_RS27790 at 6189.111 kb on - strand, within QEN71_RS27790 at 6189.139 kb on + strand, within QEN71_RS27790 at 6189.139 kb on + strand, within QEN71_RS27790 at 6189.139 kb on + strand, within QEN71_RS27790 at 6189.139 kb on + strand, within QEN71_RS27790 at 6189.139 kb on + strand, within QEN71_RS27790 at 6189.139 kb on + strand, within QEN71_RS27790 at 6189.139 kb on + strand, within QEN71_RS27790 at 6189.140 kb on - strand, within QEN71_RS27790 at 6189.271 kb on + strand at 6189.271 kb on + strand at 6189.271 kb on + strand at 6189.271 kb on + strand at 6189.271 kb on + strand at 6189.271 kb on + strand at 6189.272 kb on - strand at 6189.272 kb on - strand at 6189.272 kb on - strand at 6189.272 kb on - strand at 6189.272 kb on - strand at 6189.272 kb on - strand at 6189.272 kb on - strand at 6189.310 kb on + strand at 6189.310 kb on + strand at 6189.311 kb on - strand at 6189.311 kb on - strand at 6189.311 kb on - strand at 6189.311 kb on - strand at 6189.311 kb on - strand at 6189.335 kb on + strand at 6189.335 kb on + strand at 6189.335 kb on + strand at 6189.335 kb on + strand at 6189.336 kb on - strand at 6189.337 kb on + strand at 6189.337 kb on + strand at 6189.337 kb on + strand at 6189.337 kb on + strand at 6189.337 kb on + strand at 6189.337 kb on + strand at 6189.337 kb on + strand at 6189.337 kb on + strand at 6189.337 kb on + strand at 6189.337 kb on + strand at 6189.337 kb on + strand at 6189.337 kb on + strand at 6189.337 kb on + strand at 6189.337 kb on + strand at 6189.337 kb on + strand at 6189.337 kb on + strand at 6189.337 kb on + strand at 6189.337 kb on + strand at 6189.337 kb on + strand at 6189.337 kb on + strand at 6189.337 kb on + strand at 6189.337 kb on + strand at 6189.337 kb on + strand at 6189.337 kb on + strand at 6189.337 kb on + strand at 6189.337 kb on + strand at 6189.337 kb on + strand at 6189.337 kb on + strand at 6189.337 kb on + strand at 6189.337 kb on + strand at 6189.337 kb on + strand at 6189.337 kb on + strand at 6189.337 kb on + strand at 6189.337 kb on + strand at 6189.337 kb on + strand at 6189.337 kb on + strand at 6189.337 kb on + strand at 6189.337 kb on + strand at 6189.337 kb on + strand at 6189.337 kb on + strand at 6189.337 kb on + strand at 6189.337 kb on + strand at 6189.337 kb on + strand at 6189.337 kb on + strand at 6189.337 kb on + strand at 6189.337 kb on + strand at 6189.337 kb on + strand at 6189.337 kb on + strand at 6189.337 kb on + strand at 6189.337 kb on + strand at 6189.337 kb on + strand at 6189.337 kb on + strand at 6189.337 kb on + strand at 6189.337 kb on + strand at 6189.337 kb on + strand at 6189.337 kb on + strand at 6189.337 kb on + strand at 6189.337 kb on + strand at 6189.337 kb on + strand at 6189.338 kb on - strand at 6189.338 kb on - strand at 6189.338 kb on - strand at 6189.338 kb on - strand at 6189.338 kb on - strand at 6189.338 kb on - strand at 6189.338 kb on - strand at 6189.338 kb on - strand at 6189.338 kb on - strand at 6189.338 kb on - strand at 6189.338 kb on - strand at 6189.338 kb on - strand at 6189.338 kb on - strand at 6189.338 kb on - strand at 6189.338 kb on - strand at 6189.338 kb on - strand at 6189.338 kb on - strand at 6189.338 kb on - strand at 6189.338 kb on - strand at 6189.338 kb on - strand at 6189.338 kb on - strand at 6189.338 kb on - strand at 6189.338 kb on - strand at 6189.338 kb on - strand at 6189.338 kb on - strand at 6189.338 kb on - strand at 6189.338 kb on - strand at 6189.338 kb on - strand at 6189.338 kb on - strand at 6189.338 kb on - strand at 6189.338 kb on - strand at 6189.345 kb on + strand at 6189.345 kb on + strand at 6189.346 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_noSalt with Chloride 200 mM remove 6,187,010 + QEN71_RS27780 0.46 -0.6 6,187,623 - +0.0 6,187,730 - -0.2 6,187,730 - +0.2 6,187,730 - -1.0 6,187,754 - -0.4 6,187,870 + QEN71_RS27785 0.19 -0.8 6,187,871 - QEN71_RS27785 0.19 -0.8 6,187,871 - QEN71_RS27785 0.19 -0.8 6,188,205 - QEN71_RS27785 0.73 +0.0 6,188,206 - QEN71_RS27785 0.73 -0.6 6,188,262 - QEN71_RS27785 0.83 -1.0 6,188,262 - QEN71_RS27785 0.83 +2.0 6,188,423 + +0.4 6,188,423 + -0.6 6,188,424 - -2.2 6,188,424 - -0.2 6,188,424 - -0.8 6,188,450 - -0.9 6,188,450 - +0.0 6,188,450 - +0.0 6,188,488 + +0.8 6,188,488 + -0.6 6,188,488 + +0.0 6,188,488 + +0.2 6,188,489 - +0.0 6,188,534 + +0.6 6,188,534 + -0.3 6,188,534 + -0.2 6,188,534 + +1.6 6,188,535 - -1.6 6,188,535 - -0.1 6,188,537 + +0.1 6,188,574 + +1.5 6,188,600 + +0.2 6,188,600 + +1.0 6,188,600 + +1.3 6,188,601 - -0.6 6,188,601 - -0.6 6,188,601 - -0.4 6,188,606 + -0.3 6,188,606 + -0.6 6,188,607 - -0.3 6,188,607 - -1.2 6,188,607 - -3.1 6,188,607 - -0.4 6,188,607 - -0.2 6,188,608 + -0.4 6,188,610 + -1.0 6,188,610 + +0.1 6,188,610 + +2.6 6,188,610 + +0.4 6,188,610 + +1.5 6,188,610 + -0.2 6,188,610 + +0.0 6,188,610 + +2.7 6,188,610 + -1.3 6,188,610 + +0.4 6,188,610 + +0.2 6,188,610 + +0.4 6,188,610 + -0.0 6,188,610 + -0.6 6,188,610 + -0.3 6,188,610 + +0.2 6,188,610 + -0.1 6,188,610 + +0.0 6,188,610 + +0.3 6,188,611 - -0.2 6,188,611 - -0.2 6,188,611 - -0.5 6,188,611 - -0.7 6,188,611 - -0.2 6,188,611 - -1.6 6,188,611 - -0.8 6,188,611 - -0.3 6,188,611 - +0.5 6,188,611 - -0.6 6,188,611 - +1.9 6,188,611 - +0.5 6,188,611 - -1.3 6,188,611 - -1.0 6,188,611 - -1.0 6,188,611 - +0.4 6,188,611 - -0.7 6,188,611 - +0.2 6,188,611 - +1.4 6,188,612 + -0.0 6,188,612 + +0.1 6,188,612 + +0.5 6,188,613 - -1.0 6,188,613 - +0.0 6,188,613 - -2.1 6,188,613 - +0.2 6,188,651 + -1.5 6,188,651 + +1.2 6,188,729 + QEN71_RS27790 0.19 -0.0 6,188,729 + QEN71_RS27790 0.19 +1.7 6,188,729 + QEN71_RS27790 0.19 +0.4 6,188,729 + QEN71_RS27790 0.19 -0.3 6,188,729 + QEN71_RS27790 0.19 +0.4 6,188,729 + QEN71_RS27790 0.19 +0.5 6,188,729 + QEN71_RS27790 0.19 +1.0 6,188,729 + QEN71_RS27790 0.19 +0.1 6,188,729 + QEN71_RS27790 0.19 +1.2 6,188,729 + QEN71_RS27790 0.19 +0.2 6,188,729 + QEN71_RS27790 0.19 +0.0 6,188,729 + QEN71_RS27790 0.19 +0.9 6,188,729 + QEN71_RS27790 0.19 -0.7 6,188,729 + QEN71_RS27790 0.19 -0.6 6,188,729 + QEN71_RS27790 0.19 -0.3 6,188,729 + QEN71_RS27790 0.19 -0.1 6,188,729 + QEN71_RS27790 0.19 -0.3 6,188,730 - QEN71_RS27790 0.19 -0.7 6,188,730 - QEN71_RS27790 0.19 +0.1 6,188,730 - QEN71_RS27790 0.19 -0.8 6,188,730 - QEN71_RS27790 0.19 -0.7 6,188,730 - QEN71_RS27790 0.19 -2.4 6,188,730 - QEN71_RS27790 0.19 +0.4 6,188,730 - QEN71_RS27790 0.19 -0.4 6,188,730 - QEN71_RS27790 0.19 -0.4 6,188,730 - QEN71_RS27790 0.19 +0.3 6,188,730 - QEN71_RS27790 0.19 -0.2 6,188,730 - QEN71_RS27790 0.19 +0.8 6,188,730 - QEN71_RS27790 0.19 -1.4 6,188,730 - QEN71_RS27790 0.19 -0.8 6,188,730 - QEN71_RS27790 0.19 +0.7 6,188,730 - QEN71_RS27790 0.19 -0.2 6,188,730 - QEN71_RS27790 0.19 -0.1 6,188,730 - QEN71_RS27790 0.19 -0.8 6,188,730 - QEN71_RS27790 0.19 -0.9 6,188,730 - QEN71_RS27790 0.19 -0.8 6,188,730 - QEN71_RS27790 0.19 -1.4 6,188,730 - QEN71_RS27790 0.19 +0.1 6,188,730 - QEN71_RS27790 0.19 -0.1 6,188,730 - QEN71_RS27790 0.19 -0.6 6,188,730 - QEN71_RS27790 0.19 +1.0 6,188,730 - QEN71_RS27790 0.19 +0.6 6,188,730 - QEN71_RS27790 0.19 +0.1 6,188,730 - QEN71_RS27790 0.19 +0.0 6,188,764 + QEN71_RS27790 0.23 +0.2 6,188,765 - QEN71_RS27790 0.24 -0.6 6,188,765 - QEN71_RS27790 0.24 -0.9 6,188,765 - QEN71_RS27790 0.24 +0.1 6,188,765 - QEN71_RS27790 0.24 -0.1 6,188,765 - QEN71_RS27790 0.24 +1.3 6,188,800 + QEN71_RS27790 0.28 +1.4 6,188,801 - QEN71_RS27790 0.28 -1.0 6,188,801 - QEN71_RS27790 0.28 -0.8 6,188,801 - QEN71_RS27790 0.28 +0.3 6,188,862 + QEN71_RS27790 0.37 -0.1 6,188,863 - QEN71_RS27790 0.37 -0.1 6,188,863 - QEN71_RS27790 0.37 +0.4 6,188,863 - QEN71_RS27790 0.37 -0.5 6,189,020 - QEN71_RS27790 0.58 +0.4 6,189,020 - QEN71_RS27790 0.58 +0.4 6,189,020 - QEN71_RS27790 0.58 -0.6 6,189,020 - QEN71_RS27790 0.58 -0.6 6,189,020 - QEN71_RS27790 0.58 -1.5 6,189,097 + QEN71_RS27790 0.68 -0.4 6,189,097 + QEN71_RS27790 0.68 +1.0 6,189,097 + QEN71_RS27790 0.68 -0.6 6,189,097 + QEN71_RS27790 0.68 -0.6 6,189,097 + QEN71_RS27790 0.68 +1.7 6,189,098 - QEN71_RS27790 0.68 +0.4 6,189,098 - QEN71_RS27790 0.68 -0.7 6,189,110 + QEN71_RS27790 0.70 +0.9 6,189,111 - QEN71_RS27790 0.70 -0.7 6,189,139 + QEN71_RS27790 0.74 -0.7 6,189,139 + QEN71_RS27790 0.74 -0.3 6,189,139 + QEN71_RS27790 0.74 +0.0 6,189,139 + QEN71_RS27790 0.74 -1.1 6,189,139 + QEN71_RS27790 0.74 +1.3 6,189,139 + QEN71_RS27790 0.74 -0.3 6,189,139 + QEN71_RS27790 0.74 -0.1 6,189,140 - QEN71_RS27790 0.74 +0.2 6,189,271 + -1.1 6,189,271 + -0.1 6,189,271 + -0.1 6,189,271 + +0.5 6,189,271 + +1.3 6,189,271 + -0.2 6,189,272 - -0.7 6,189,272 - +0.7 6,189,272 - +0.1 6,189,272 - -0.6 6,189,272 - -0.3 6,189,272 - -0.6 6,189,272 - -0.8 6,189,310 + +0.1 6,189,310 + -0.7 6,189,311 - -1.0 6,189,311 - -2.1 6,189,311 - -0.0 6,189,311 - -0.2 6,189,311 - +0.6 6,189,335 + +0.6 6,189,335 + +0.0 6,189,335 + -1.3 6,189,335 + -0.4 6,189,336 - -3.3 6,189,337 + +0.7 6,189,337 + -1.0 6,189,337 + +0.5 6,189,337 + +0.0 6,189,337 + -0.6 6,189,337 + -0.9 6,189,337 + -0.6 6,189,337 + -1.1 6,189,337 + +0.4 6,189,337 + +0.2 6,189,337 + -0.2 6,189,337 + +0.4 6,189,337 + +1.1 6,189,337 + +0.0 6,189,337 + -0.0 6,189,337 + -0.8 6,189,337 + -0.8 6,189,337 + +1.6 6,189,337 + +1.3 6,189,337 + +0.0 6,189,337 + +0.7 6,189,337 + +1.2 6,189,337 + +2.3 6,189,337 + -0.1 6,189,337 + +0.3 6,189,337 + +0.3 6,189,337 + +0.0 6,189,337 + -0.4 6,189,337 + -0.3 6,189,337 + -0.1 6,189,337 + +0.7 6,189,337 + +0.2 6,189,337 + -0.3 6,189,337 + -1.2 6,189,337 + -0.7 6,189,337 + +0.1 6,189,337 + -0.7 6,189,337 + -0.0 6,189,337 + +0.4 6,189,337 + -0.6 6,189,337 + +0.2 6,189,337 + -0.3 6,189,337 + +0.0 6,189,337 + -0.2 6,189,337 + +0.0 6,189,337 + -1.4 6,189,337 + +0.1 6,189,337 + -1.1 6,189,337 + +0.5 6,189,337 + +0.6 6,189,337 + -0.3 6,189,337 + +0.2 6,189,337 + -0.8 6,189,337 + -0.8 6,189,337 + -0.0 6,189,337 + +0.2 6,189,337 + +0.2 6,189,337 + -1.1 6,189,337 + +0.7 6,189,338 - -1.0 6,189,338 - -3.4 6,189,338 - -1.2 6,189,338 - +0.1 6,189,338 - -1.8 6,189,338 - -2.5 6,189,338 - -0.3 6,189,338 - -0.8 6,189,338 - -2.2 6,189,338 - -1.8 6,189,338 - -2.0 6,189,338 - -2.6 6,189,338 - -1.4 6,189,338 - -1.3 6,189,338 - -0.1 6,189,338 - -1.2 6,189,338 - -2.1 6,189,338 - -2.6 6,189,338 - -1.0 6,189,338 - -0.7 6,189,338 - +0.0 6,189,338 - -0.3 6,189,338 - -0.1 6,189,338 - -1.8 6,189,338 - -1.8 6,189,338 - -0.2 6,189,338 - -1.7 6,189,338 - +0.3 6,189,338 - -2.4 6,189,338 - -1.8 6,189,338 - -1.4 6,189,345 + -0.6 6,189,345 + -1.3 6,189,346 - -2.2
Or see this region's nucleotide sequence