Experiment: LB_noSalt with Chloride 200 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt QEN71_RS14405 and QEN71_RS14410 are separated by 40 nucleotides QEN71_RS14410 and QEN71_RS14415 overlap by 4 nucleotides QEN71_RS14415 and QEN71_RS14420 overlap by 4 nucleotides QEN71_RS14420 and QEN71_RS14425 are separated by 23 nucleotides
QEN71_RS14405: QEN71_RS14405 - methylamine dehydrogenase accessory protein MauD, at 3,212,658 to 3,213,284
_RS14405
QEN71_RS14410: QEN71_RS14410 - methylamine dehydrogenase light chain, at 3,213,325 to 3,213,870
_RS14410
QEN71_RS14415: QEN71_RS14415 - cytochrome c, at 3,213,867 to 3,214,364
_RS14415
QEN71_RS14420: QEN71_RS14420 - cytochrome c, at 3,214,361 to 3,214,924
_RS14420
QEN71_RS14425: QEN71_RS14425 - aldehyde dehydrogenase family protein, at 3,214,948 to 3,216,453
_RS14425
Position (kb)
3213
3214
3215 Strain fitness (log2 ratio)
-1
0
1
2 at 3213.069 kb on + strand, within QEN71_RS14405 at 3213.099 kb on + strand, within QEN71_RS14405 at 3213.120 kb on + strand, within QEN71_RS14405 at 3213.466 kb on + strand, within QEN71_RS14410 at 3213.550 kb on + strand, within QEN71_RS14410 at 3213.550 kb on + strand, within QEN71_RS14410 at 3213.560 kb on - strand, within QEN71_RS14410 at 3213.685 kb on + strand, within QEN71_RS14410 at 3213.686 kb on - strand, within QEN71_RS14410 at 3213.769 kb on + strand, within QEN71_RS14410 at 3213.769 kb on + strand, within QEN71_RS14410 at 3213.770 kb on - strand, within QEN71_RS14410 at 3213.770 kb on - strand, within QEN71_RS14410 at 3213.770 kb on - strand, within QEN71_RS14410 at 3213.826 kb on + strand at 3213.826 kb on + strand at 3213.827 kb on - strand at 3213.879 kb on + strand at 3213.879 kb on + strand at 3213.964 kb on - strand, within QEN71_RS14415 at 3214.059 kb on + strand, within QEN71_RS14415 at 3214.072 kb on - strand, within QEN71_RS14415 at 3214.072 kb on - strand, within QEN71_RS14415 at 3214.158 kb on + strand, within QEN71_RS14415 at 3214.159 kb on - strand, within QEN71_RS14415 at 3214.221 kb on + strand, within QEN71_RS14415 at 3214.222 kb on - strand, within QEN71_RS14415 at 3214.682 kb on + strand, within QEN71_RS14420 at 3214.683 kb on - strand, within QEN71_RS14420 at 3214.803 kb on - strand, within QEN71_RS14420 at 3214.804 kb on + strand, within QEN71_RS14420 at 3214.805 kb on - strand, within QEN71_RS14420 at 3214.805 kb on - strand, within QEN71_RS14420 at 3214.805 kb on - strand, within QEN71_RS14420 at 3215.338 kb on + strand, within QEN71_RS14425 at 3215.338 kb on + strand, within QEN71_RS14425
Per-strain Table
Position Strand Gene LocusTag Fraction LB_noSalt with Chloride 200 mM remove 3,213,069 + QEN71_RS14405 0.66 -0.4 3,213,099 + QEN71_RS14405 0.70 -0.2 3,213,120 + QEN71_RS14405 0.74 -0.9 3,213,466 + QEN71_RS14410 0.26 -1.6 3,213,550 + QEN71_RS14410 0.41 -0.8 3,213,550 + QEN71_RS14410 0.41 -0.8 3,213,560 - QEN71_RS14410 0.43 -0.2 3,213,685 + QEN71_RS14410 0.66 -0.4 3,213,686 - QEN71_RS14410 0.66 -0.8 3,213,769 + QEN71_RS14410 0.81 +0.3 3,213,769 + QEN71_RS14410 0.81 -0.5 3,213,770 - QEN71_RS14410 0.82 +0.3 3,213,770 - QEN71_RS14410 0.82 +0.7 3,213,770 - QEN71_RS14410 0.82 -0.7 3,213,826 + -0.9 3,213,826 + -0.6 3,213,827 - -1.1 3,213,879 + +1.2 3,213,879 + +0.2 3,213,964 - QEN71_RS14415 0.19 -0.5 3,214,059 + QEN71_RS14415 0.39 +0.3 3,214,072 - QEN71_RS14415 0.41 -0.4 3,214,072 - QEN71_RS14415 0.41 -1.0 3,214,158 + QEN71_RS14415 0.58 -0.8 3,214,159 - QEN71_RS14415 0.59 -0.3 3,214,221 + QEN71_RS14415 0.71 -1.1 3,214,222 - QEN71_RS14415 0.71 -0.5 3,214,682 + QEN71_RS14420 0.57 +0.1 3,214,683 - QEN71_RS14420 0.57 +1.2 3,214,803 - QEN71_RS14420 0.78 +0.0 3,214,804 + QEN71_RS14420 0.79 +2.5 3,214,805 - QEN71_RS14420 0.79 +0.0 3,214,805 - QEN71_RS14420 0.79 +0.1 3,214,805 - QEN71_RS14420 0.79 -1.2 3,215,338 + QEN71_RS14425 0.26 -0.7 3,215,338 + QEN71_RS14425 0.26 -0.4
Or see this region's nucleotide sequence