Experiment: LB_noSalt with Chloride 200 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt QEN71_RS08135 and QEN71_RS08140 are separated by 19 nucleotides QEN71_RS08140 and QEN71_RS08145 are separated by 27 nucleotides QEN71_RS08145 and QEN71_RS08150 are separated by 756 nucleotides
QEN71_RS08135: QEN71_RS08135 - hypothetical protein, at 1,801,116 to 1,801,268
_RS08135
QEN71_RS08140: QEN71_RS08140 - 2-hydroxyacid dehydrogenase, at 1,801,288 to 1,802,220
_RS08140
QEN71_RS08145: QEN71_RS08145 - OsmC family protein, at 1,802,248 to 1,802,727
_RS08145
QEN71_RS08150: QEN71_RS08150 - sigma 54-interacting transcriptional regulator, at 1,803,484 to 1,804,827
_RS08150
Position (kb)
1802
1803 Strain fitness (log2 ratio)
-2
-1
0
1 at 1801.256 kb on - strand at 1801.578 kb on + strand, within QEN71_RS08140 at 1801.579 kb on - strand, within QEN71_RS08140 at 1801.796 kb on - strand, within QEN71_RS08140 at 1801.847 kb on + strand, within QEN71_RS08140 at 1802.054 kb on - strand, within QEN71_RS08140 at 1802.311 kb on + strand, within QEN71_RS08145 at 1802.355 kb on - strand, within QEN71_RS08145 at 1802.428 kb on + strand, within QEN71_RS08145 at 1802.429 kb on - strand, within QEN71_RS08145 at 1802.429 kb on - strand, within QEN71_RS08145 at 1802.492 kb on - strand, within QEN71_RS08145 at 1802.862 kb on - strand at 1802.899 kb on - strand at 1802.903 kb on - strand at 1802.910 kb on + strand at 1802.910 kb on + strand at 1802.910 kb on + strand at 1802.910 kb on + strand at 1802.910 kb on + strand at 1802.910 kb on + strand at 1802.910 kb on + strand at 1802.910 kb on + strand at 1802.910 kb on + strand at 1802.911 kb on - strand at 1802.911 kb on - strand at 1802.917 kb on - strand at 1802.917 kb on - strand at 1802.917 kb on - strand at 1802.920 kb on + strand at 1802.920 kb on + strand at 1802.920 kb on + strand at 1802.921 kb on - strand at 1802.921 kb on - strand at 1802.923 kb on + strand at 1802.982 kb on - strand at 1803.042 kb on - strand at 1803.066 kb on - strand at 1803.101 kb on + strand at 1803.116 kb on - strand at 1803.116 kb on - strand at 1803.117 kb on + strand at 1803.197 kb on - strand at 1803.204 kb on + strand at 1803.204 kb on + strand at 1803.205 kb on - strand at 1803.205 kb on - strand at 1803.216 kb on - strand at 1803.313 kb on + strand at 1803.313 kb on + strand at 1803.314 kb on - strand at 1803.340 kb on - strand at 1803.445 kb on - strand at 1803.449 kb on - strand at 1803.449 kb on - strand at 1803.491 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_noSalt with Chloride 200 mM remove 1,801,256 - +0.4 1,801,578 + QEN71_RS08140 0.31 -0.1 1,801,579 - QEN71_RS08140 0.31 +1.7 1,801,796 - QEN71_RS08140 0.54 +0.9 1,801,847 + QEN71_RS08140 0.60 +1.1 1,802,054 - QEN71_RS08140 0.82 +1.6 1,802,311 + QEN71_RS08145 0.13 -0.8 1,802,355 - QEN71_RS08145 0.22 -1.6 1,802,428 + QEN71_RS08145 0.38 +0.2 1,802,429 - QEN71_RS08145 0.38 -0.2 1,802,429 - QEN71_RS08145 0.38 -1.4 1,802,492 - QEN71_RS08145 0.51 -0.2 1,802,862 - +0.1 1,802,899 - +0.4 1,802,903 - +1.6 1,802,910 + -0.6 1,802,910 + -1.5 1,802,910 + +0.2 1,802,910 + -1.1 1,802,910 + -0.7 1,802,910 + +0.6 1,802,910 + -1.1 1,802,910 + -1.9 1,802,910 + +0.9 1,802,911 - +0.1 1,802,911 - -0.6 1,802,917 - -0.7 1,802,917 - -0.5 1,802,917 - -0.7 1,802,920 + -1.3 1,802,920 + -0.1 1,802,920 + +0.0 1,802,921 - +0.2 1,802,921 - -1.0 1,802,923 + +1.1 1,802,982 - -0.3 1,803,042 - -1.1 1,803,066 - -0.7 1,803,101 + -1.5 1,803,116 - +0.2 1,803,116 - -0.2 1,803,117 + -1.4 1,803,197 - +0.4 1,803,204 + +0.7 1,803,204 + +0.5 1,803,205 - -0.7 1,803,205 - -0.5 1,803,216 - -0.2 1,803,313 + -1.1 1,803,313 + -2.5 1,803,314 - -0.7 1,803,340 - -0.8 1,803,445 - -0.3 1,803,449 - +0.3 1,803,449 - -1.2 1,803,491 + +0.3
Or see this region's nucleotide sequence