Experiment: LB_noSalt with Chloride 200 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt QEN71_RS03450 and QEN71_RS03455 are separated by 63 nucleotides QEN71_RS03455 and QEN71_RS03460 are separated by 1 nucleotides
QEN71_RS03450: QEN71_RS03450 - efflux RND transporter periplasmic adaptor subunit, at 767,818 to 769,071
_RS03450
QEN71_RS03455: QEN71_RS03455 - multidrug efflux RND transporter permease subunit, at 769,135 to 772,395
_RS03455
QEN71_RS03460: QEN71_RS03460 - efflux transporter outer membrane subunit, at 772,397 to 773,869
_RS03460
Position (kb)
769
770
771
772
773 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 768.222 kb on - strand, within QEN71_RS03450 at 769.369 kb on + strand at 769.369 kb on + strand at 769.369 kb on + strand at 769.369 kb on + strand at 769.369 kb on + strand at 769.369 kb on + strand at 769.369 kb on + strand at 769.369 kb on + strand at 769.370 kb on - strand at 769.370 kb on - strand at 769.370 kb on - strand at 769.370 kb on - strand at 769.370 kb on - strand at 769.370 kb on - strand at 771.916 kb on + strand, within QEN71_RS03455 at 772.483 kb on - strand at 772.483 kb on - strand at 772.488 kb on + strand at 772.488 kb on + strand at 772.500 kb on - strand at 772.500 kb on - strand at 772.517 kb on + strand at 772.517 kb on + strand at 772.517 kb on + strand at 772.517 kb on + strand at 772.517 kb on + strand at 772.517 kb on + strand at 772.517 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_noSalt with Chloride 200 mM remove 768,222 - QEN71_RS03450 0.32 +1.7 769,369 + -0.4 769,369 + -0.5 769,369 + -1.2 769,369 + -1.2 769,369 + +1.7 769,369 + -0.8 769,369 + +0.3 769,369 + +0.4 769,370 - -0.0 769,370 - +0.1 769,370 - -2.4 769,370 - +0.7 769,370 - -0.3 769,370 - -1.6 771,916 + QEN71_RS03455 0.85 -1.5 772,483 - +0.6 772,483 - -1.2 772,488 + -0.3 772,488 + +0.9 772,500 - -1.0 772,500 - -0.4 772,517 + +0.1 772,517 + +0.4 772,517 + +1.8 772,517 + -2.3 772,517 + -0.3 772,517 + -0.7 772,517 + +1.7
Or see this region's nucleotide sequence